A cryopreservation protocol for embryos of the endangered species Zizania texana.
ABSTRACT Seeds of the endangered species Zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. Excised embryos can be cryopreserved using solution-based cryoprotection protocols. Survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g H2O/g dry mass.
Chapter: Cryopreserving Vegetatively Propagated Tropical Crops - The Case of Dioscorea Species and Solenostemon rotundifolius03/2012; , ISBN: 978-953-51-0191-8