Article

A cryopreservation protocol for embryos of the endangered species Zizania texana.

USDA-ARS National Center for Genetic Resources, 1111 S. Mason Street, Fort Collins, Colorado, USA.
Cryo letters (impact factor: 1.25). 05/2002; 23(5):291-8.
Source: PubMed

ABSTRACT Seeds of the endangered species Zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. Excised embryos can be cryopreserved using solution-based cryoprotection protocols. Survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g H2O/g dry mass.

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Keywords

bathing
 
concentrations
 
cryoprotectant solutions
 
endangered species Zizania texana
 
Excised embryos
 
preculturing embryos
 
remaining genetic diversity
 
solution-based cryoprotection protocols
 
water contents