Article

The effect of exercise on brain antioxidant status of diabetic rats.

Department of Physiology, Faculty of Medicine, Akdeniz University, Arapsuyu, 07070 Antalya, Turkey.
Diabetes & Metabolism (impact factor: 2.41). 12/2002; 28(5):377-84.
Source: PubMed

ABSTRACT To investigate the effect of exercise on brain antioxidant status of diabetic rats.
Wistar rats were divided into four groups: Control (C), exercise (CE), diabetic (D), and diabetic+exercise (DE). Diabetes was induced by single administration of streptozotocin (60 mg/kg). We used an aerobic exercise program for 8 weeks of CE and DE rats. After the end of the experimental period, Cu, Zn-superoxide dismutase (Cu, Zn-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), xanthine dehydrogenase (XDH) and xanthine oxidase (XO) activities and thiobarbituric acid reactive substances (TBARS) levels of brain were measured.
Diabetes caused significant reduction of brain Cu, Zn-SOD and GSH-Px activities in the D and DE groups. CAT activity was decreased only in the D group. Exercise did not alter CAT activity of brain, whereas markedly increased Cu, Zn-SOD activity in the DE group. In contrast to diabetes-related decrease in the activity of Cu, Zn-SOD, increase in the XO and GSH-Px activities were observed in the DE group compared with the D group. XDH activity was significantly reduced in two exercise groups according to the control rats, but the decrease was not accompanied with the activity of XO elevation in all groups. Increase in the XO activity and decrease in the XDH activity in the DE rats have revealed that diabetes and exercise have potentially effect in free radical production. On the other hand, TBARS levels were found to be elevated in all diabetic animals.
Our results show that aerobic exercise did not affect lipid peroxidation of brain, but in diabetic condition improved antioxidant defence.

0 0
 · 
0 Bookmarks
 · 
34 Views
  • Source
    Article: The effect of n-acetylcysteine and deferoxamine on exercise-induced oxidative damage in striatum and hippocampus of mice.
    Aderbal S Aguiar, Talita Tuon, Fernanda S Soares, Luís Gustavo C da Rocha, Paulo César Silveira, Ricardo A Pinho
    [show abstract] [hide abstract]
    ABSTRACT: The aim of this study was to analyze the effects of intense exercise on brain redox status, associated with antioxidant supplementation of N-acetylcysteine (NAC), deferoxamine (DFX) or a combination of both. Seventy-two C57BL-6 adult male mice were randomly assigned to 8 groups: control, NAC, DFX, NAC plus DFX, exercise, exercise with NAC, exercise with DFX, and exercise with NAC plus DFX. They were given antioxidant supplementation, exercise training on a treadmill for 12 weeks, and sacrificed 48 h after the last exercise session. Training significantly increased (P < 0.05) soleus citrate synthase (CS) activity when compared to control. Blood lactate levels classified the exercise as intense. Exercise significantly increased (P < 0.05) oxidation of biomolecules and superoxide dismutase activity in striatum and hippocampus. Training significantly increased (P < 0.05) catalase activity in striatum. NAC and DFX supplementation significantly protected (P < 0.05) against oxidative damage. These results indicate intense exercise as oxidant and NAC and DFX as antioxidant to the hippocampus and the striatum.
    Neurochemical Research 06/2008; 33(5):729-36. · 2.24 Impact Factor
  • Source
    Article: Muscle Fatigue and Cognition: What is the Link?
    Tali Kobilo, Henriette van Praag
    Frontiers in physiology. 01/2012; 3:14.
  • Source
    Article: Impact of treadmill running and sex on hippocampal neurogenesis in the mouse model of amyotrophic lateral sclerosis.
    Xiaoxing Ma, Mazen J Hamadeh, Brain R Christie, Jane A Foster, Mark A Tarnopolsky
    [show abstract] [hide abstract]
    ABSTRACT: Hippocampal neurogenesis in the subgranular zone (SGZ) of dentate gyrus (DG) occurs throughout life and is regulated by pathological and physiological processes. The role of oxidative stress in hippocampal neurogenesis and its response to exercise or neurodegenerative diseases remains controversial. The present study was designed to investigate the impact of oxidative stress, treadmill exercise and sex on hippocampal neurogenesis in a murine model of heightened oxidative stress (G93A mice). G93A and wild type (WT) mice were randomized to a treadmill running (EX) or a sedentary (SED) group for 1 or 4 wk. Immunohistochemistry was used to detect bromodeoxyuridine (BrdU) labeled proliferating cells, surviving cells, and their phenotype, as well as for determination of oxidative stress (3-NT; 8-OHdG). BDNF and IGF1 mRNA expression was assessed by in situ hybridization. Results showed that: (1) G93A-SED mice had greater hippocampal neurogenesis, BDNF mRNA, and 3-NT, as compared to WT-SED mice. (2) Treadmill running promoted hippocampal neurogenesis and BDNF mRNA content and lowered DNA oxidative damage (8-OHdG) in WT mice. (3) Male G93A mice showed significantly higher cell proliferation but a lower level of survival vs. female G93A mice. We conclude that G93A mice show higher hippocampal neurogenesis, in association with higher BDNF expression, yet running did not further enhance these phenomena in G93A mice, probably due to a 'ceiling effect' of an already heightened basal levels of hippocampal neurogenesis and BDNF expression.
    PLoS ONE 01/2012; 7(4):e36048. · 4.09 Impact Factor

Show more

Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

aerobic exercise
 
aerobic exercise program
 
antioxidant defence
 
brain Cu
 
control rats
 
D group
 
DE group
 
DE groups
 
DE rats
 
diabetic animals
 
diabetic condition
 
diabetic rats
 
exercise groups
 
experimental period
 
glutathione peroxidase
 
single administration
 
thiobarbituric acid reactive substances
 
xanthine dehydrogenase
 
XDH activity
 
Zn-SOD activity