Article

[Involvement of heparanase in human abdominal aortic aneurysm].

Research Institute of Vascular Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Zhonghua yi xue za zhi 10/2002; 82(20):1378-80. pp.1378-80
Source: PubMed

ABSTRACT To determine whether heparanase might participate the formation of abdominal aortic aneurysms (AAA) and explore a novel pathogenesis for it.
Total RNA was extracted from 20 tissue samples of human infrarenal abdominal aortic aneurysm (AAA) and 5 samples of normal aorta (NA), all resected in operation, and 5 samples of primary hepatocellular carcinoma. Northern blot was used to evaluate the expression of heparanase. Immunohistochemistry and computer-assisted image analysis were used to assess the expression of heparanase and its substrate heparan sulfate proteoglycan protein (HSGP) and microvessel hyperplasia.
Semi-quantitative analysis showed that the expression of heparanase in AAA was significantly higher than that in normal abdominal aorta (P < 0.01), but equivalent to that in primary hepatocellular carcinoma (P > 0.05). However, the expression of HSPG was lowered in AAA and was significantly negatively correlated with the expression of heparanase and microvessel density. Both of heparanase and HSPG were mainly located in adventitia and intima of AAA walls.
Heparanase participates in the structure damage and remodeling of abdominal aorta at matrix level, which contributes to AAA formation.

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Keywords

20 tissue samples
 
5 samples
 
AAA formation
 
AAA walls
 
abdominal aorta
 
abdominal aortic aneurysms
 
computer-assisted image analysis
 
heparanase
 
Heparanase participates
 
human infrarenal abdominal aortic aneurysm
 
intima
 
matrix level
 
microvessel hyperplasia
 
normal abdominal aorta
 
Northern blot
 
novel pathogenesis
 
Semi-quantitative analysis
 
structure damage
 
substrate heparan sulfate proteoglycan protein
 
Total RNA
 

Jianqiu Wu