Article

Early effects of gliadin on enterocyte intracellular signalling involved in intestinal barrier function.

Department of Biomedical Sciences and Biotechnology, 2nd Pediatric Clinic, University of Cagliari, Cagliari, Italy.
Gut (Impact Factor: 13.32). 03/2003; 52(2):218-23.
Source: PubMed

ABSTRACT Despite the progress made in understanding the immunological aspects of the pathogenesis of coeliac disease (CD), the early steps that allow gliadin to cross the intestinal barrier are still largely unknown. The aim of this study was to establish whether gliadin activates a zonulin dependent enterocyte intracellular signalling pathway(s) leading to increased intestinal permeability.
The effect of gliadin on the enterocyte actin cytoskeleton was studied on rat intestinal epithelial (IEC-6) cell cultures by fluorescence microscopy and spectrofluorimetry. Zonulin concentration was measured on cell culture supernatants by enzyme linked immunosorbent assay. Transepithelial intestinal resistance (Rt) was measured on ex vivo intestinal tissues mounted in Ussing chambers.
Incubation of cells with gliadin led to a reversible protein kinase C (PKC) mediated actin polymerisation temporarily coincident with zonulin release. A significant reduction in Rt was observed after gliadin addition on rabbit intestinal mucosa mounted in Ussing chambers. Pretreatment with the zonulin inhibitor FZI/0 abolished the gliadin induced actin polymerisation and Rt reduction but not zonulin release.
Gliadin induces zonulin release in intestinal epithelial cells in vitro. Activation of the zonulin pathway by PKC mediated cytoskeleton reorganisation and tight junction opening leads to a rapid increase in intestinal permeability.

0 Bookmarks
 · 
100 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This article presents a method for long-term autonomous micro-aerial vehicle (MAV) localization and position stabilization. The proposed method extends MAV proprietary stabilization based on inertial sensor or optical flow processing, without use of an external positioning system. The method extracts visual features from the images captured by a down-looking camera mounted under the MAV and matching these to previously observed features. Due to its precision and reliability, the method is well suited for stabilization of MAVs acting in closely cooperating compact teams with small mutual distances between team members. Performance of the proposed method is demonstrated by experiments on a quad-copter equipped with all necessary sensors and computers for the autonomous operation.
    2014 International Conference on Unmanned Aircraft Systems (ICUAS); 05/2014
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: 1. Overview of Biomarkers for Diagnosis and Monitoring of Celiac Disease 2. Cystatin C: A Kidney Function Biomarker 3. Procalcitonin: Potential Role in Diagnosis and Management 4. Manganese Superoxide Dismutase and Oxidative Stress Modulation 5. Selenium and Selenium-Dependent Antioxidants in Chronic Kidney Disease 6. Lipidomics:New Insight Into Kidney Disease
    Advances in clinical chemistry 02/2015; 68. · 4.30 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Refractory coeliac disease (RCD) is characterised by recurrent or persistent malabsorptive symptoms and villous atrophy, despite strict adherence to a gluten-free diet for at least 6 months and where other causes of malabsorption including malignancy have been excluded. There is limited evidence and guidance on the effective management of these patients. We describe a case of severe RCD in our hospital, with symptoms controlled effectively only by total parenteral nutrition (TPN). This 68-year-old woman initially presented to the clinic with persistent non-bloody diarrhoea and vomiting. A diagnosis of coeliac disease was confirmed with a positive tissue transglutaminase assay and histology. A strict gluten-free diet was ineffective and she represented 6 months later with 13 kg weight loss (16.7%), ongoing abdominal pain and diarrhoea, with bowels opening 16 times a day. She was oedematous, had an albumin of 12 g/l and required hospital admission. She was treated for pancreatic insufficiency and presumptively for small bowel bacterial overgrowth with no resolution of symptoms. We ruled out infectious causes and investigated for small bowel malignancy; all results were negative. Small bowel enteroscopy showed ulcerative jejunitis. She was given 5 days of TPN, following which her symptoms improved and albumin normalised. This was sustained with symptom resolution and weight gain seen at follow-up. TPN successfully and rapidly induced remission in this case. Thus, a short period of TPN should be considered as a potential component of management in patients with severe RCD.
    Case Reports in Gastroenterology 01/2014; 8(2):297-303. DOI:10.1159/000368394

Full-text (2 Sources)

Download
81 Downloads
Available from
Jun 5, 2014