Article

Morphological quantification of emphysema in small human lung specimens: comparison of methods and relation with clinical data.

Department of Biochemistry, NCMLS, University Medical Centre, Nijmegen, The Netherlands.
Modern Pathology (Impact Factor: 6.36). 01/2003; 16(1):1-7.
Source: PubMed

ABSTRACT Small human lung specimens are frequently used for cell biological studies of the pathogenesis of emphysema. In general, lung function and other clinical parameters are used to establish the presence and severity of emphysema/chronic obstructive pulmonary disease without morphological analysis of the specimens under investigation. In this study we compared three morphological methods to analyze emphysema, and evaluated whether clinical data correlate with the morphological data of individual lung samples. A total of 306 lung specimens from resected lung(lobes) from 221 patients were inflated and characterized using three morphological parameters: the Destructive Index, the Mean Linear Intercept, and Section Assessment. Morphological data were related to each other, to lung function data, and to smoking behavior. Significant correlations (P < .001) were observed between Section Assessment and Destructive Index (r = 0.92), Mean Linear Intercept with Destructive Index (r = 0.69) and Mean Linear Intercept with Section Assessment (r = 0.65). Section Assessment, being much less time consuming than Mean Linear Intercept and Destructive Index, is the parameter of choice for initial analysis. Destructive Index is the most sensitive parameter. There was a significant (P < .001), but weak correlation for all three parameters with the diffusion capacity for CO (K(CO)) (Destructive Index: r = -0.28; Mean Linear Intercept: r = -0.34; Section Assessment: r = -0.32), and with FEV(1)/IVC (Destructive Index: r = -0.29; Mean Linear Intercept: r = -0.33; Section Assessment: r = -0.28), but not with other lung function parameters. A significant difference (P < .05) between (ex-) smokers and never-smokers was observed for Destructive Index and Section Assessment. It is concluded that the application of the three morphological parameters represents a useful method to characterize emphysematous lesions in a (semi-)quantitative manner in small human lung specimens, and that Section Assessment is a suitable and fast method for initial screening. The extent of emphysema of individual lung specimens should be established by means of morphometry, rather than lung function data.

0 Bookmarks
 · 
83 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Respiratory infections in early life can lead to chronic respiratory disease. Chlamydia infections are common causes of respiratory disease, particularly pneumonia in neonates, and are linked to permanent reductions in pulmonary function and the induction of asthma. However, the immune responses that protect against early-life infection and the mechanisms that lead to chronic lung disease are incompletely understood. Here we identify novel roles for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in promoting Chlamydia respiratory infection-induced pathology in early life, and subsequent chronic lung disease. By infecting TRAIL-deficient neonatal mice and using neutralizing antibodies against this factor and its receptors in wild-type mice, we demonstrate that TRAIL is critical in promoting infection-induced histopathology, inflammation, and mucus hypersecretion, as well as subsequent alveolar enlargement and impaired lung function. This suggests that therapeutic agents that target TRAIL or its receptors may be effective treatments for early-life respiratory infections and associated chronic lung disease.Mucosal Immunology advance online publication, 18 September 2013; doi:10.1038/mi.2013.65.
    Mucosal Immunology 09/2013; · 7.54 Impact Factor
  • Pharmacopsychiatry 12/2007; 40. · 2.17 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background:Bronchopulmonary Dysplasia (BPD) is a multifactor chronic lung disease that mainly affects premature infants. In this study, we investigate the preventive effects of Astragalus polysaccharides (APS) on BPD, and explored its potential molecular mechanisms.Methods:Lung tissues of newborn SD rats, came from control group, room air plus low-dose APS group, room air plus high-dose APS group, BPD model group, low-dose APS group (20mg/kg·d) and high-dose APS group (40mg/kg·d) were gained at the 4th, 10th and 14th day of its life. The pathomorphological change was evaluated by hematoxylin-eosin staining. The content level of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by the assay kit. Moreover, the protein and/or mRNA expression level of NF-κBp65, CD31, ICAM-1 and TNF- α were also detected by corresponding method.Results:APS decreased the inflammatory cells infiltrating comparing with BPD group. For APS group, the activity of SOD was increased and the content of MDA was reduced compared with BPD group at any time point. The protein and mRNA expression level of NF-κBp65, ICAM-1, TNF-α were all decreased while the protein expression level of CD31 was increased in APS treated group with the most significantly difference of the high dose group (P<0.01) compared with BPD group after birth on 4d, 10d and 14d.Conclusion:APS can reduce airway remodeling and alveolar damage by its modulation of inflammatory mediators and antioxidation, suggesting some protective effects on BPD of neonatal rats.Pediatric Research (2014); doi:10.1038/pr.2014.107.
    Pediatric Research 07/2014; · 2.84 Impact Factor

Full-text (2 Sources)

Download
216 Downloads
Available from
Jun 1, 2014