Article

Rearranged gene order between pig and human in a QTL region on SSC 7.

Laboratoire de Génétique Cellulaire, INRA, BP 27, 31326 Castanet-Tolosan, France.
Mammalian Genome (impact factor: 2.89). 02/2003; 14(1):71-80. DOI:10.1007/s00335-002-3034-1 pp.71-80
Source: PubMed

ABSTRACT On porcine Chromosome 7, the region surrounding the MHC region contains QTL influencing many traits including growth, back fat thickness, and carcass composition. Towards the identification of the responsible gene(s), this article describes an increase of density of the radiated hybrid map of SSC 7 in the q11-q14 region and the comparative analysis of gene order on the porcine RH map and human genome assembly. Adding 24 new genes in this region, we were able to build a framework map that fills in gaps on the previous maps. The new software Carthagene was used to build a robust framework in this region. Comparative analysis of human and porcine maps revealed a global conservation of gene order and of distances between genes. A rearranged fragment of around 3.7 Mb was, however, found in the pig approximately 20 Mb upstream from the expected location on the basis of the human map. This rearrangement, found by RH mapping on the IMpRH 7.000 rads panel, has been confirmed by two-color FISH and by mapping on the high resolution IMNpRH2 12.000 rads panel. The rearranged fragment contains two microsatellites found at the most likely QTL location in the INRA QTL experiment. It also contains the BMP5 gene, which, together with CLPS, could be considered as a possible candidate.

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Keywords

24 new genes
 
carcass composition
 
Comparative analysis
 
expected location
 
fat thickness
 
gene order
 
global conservation
 
human genome assembly
 
INRA QTL experiment
 
likely QTL location
 
MHC region
 
porcine Chromosome 7
 
porcine maps
 
porcine RH map
 
possible candidate
 
previous maps
 
QTL influencing
 
rearranged fragment
 
resolution IMNpRH2 12.000 rads panel
 
responsible gene(s)