Cytotoxic naphthoquinones and plumbagic acid glucosides from Plumbago zeylanica.
ABSTRACT Two plumbagic acid glucosides, 3'-O-beta-glucopyranosyl plumbagic acid and 3'-O-beta-glucopyranosyl plumbagic acid methylester along with five naphthoquinones (plumbagin, chitranone, maritinone, elliptinone and isoshinanolone), and five coumarins (seselin, 5-methoxyseselin, suberosin, xanthyletin and xanthoxyletin) were isolated from the roots of Plumbago zeylanica. All coumarins were not previously found in this plant. Cytotoxicity of these compounds to various tumor cells lines was evaluated, and plumbagin significantly suppressed growth of Raji, Calu-1, HeLa, and Wish tumor cell lines.
Article: Noteworthy Secondary Metabolites Naphthoquinones-their Occurrence, Pharmacological Properties and Analysis[show abstract] [hide abstract]
ABSTRACT: Chemical investigation of many bacterial and fungal, as well as plant species has revealed the presence of in-teresting compounds derived from naphthalene – 1,4-naphthoquinones and rarely also 1,2-naphthoquinones. They were detected in many species of families Bignoniaceae, Droseraceae, Plumbaginace, Boraginaceae, Juglandaceae as well as in species of small families, such as Dioncophyllaceae or Acanthaceae. Naphthoquinones have very interesting spectrum of biological actions, including antibiotic, antiviral, anti-inflammatory, antipyretic, antiproliferative and cytotoxic effects. Because of these properties the plants containing them are used in folk medicines, mainly by natives in Asia, where espe-cially Chinese medicine uses aerial as well as subterranean parts of these plants for hundreds years, and South America. The utilizing of naphthoquinones for medicinal purposes and their occurrence in nature is reviewed and discussed. Moreover, we review analytical techniques using for their analysis.
Article: Expression, purification, and characterization of anti-plumbagin single-chain variable fragment antibody in Sf9 insect cell.[show abstract] [hide abstract]
ABSTRACT: Plumbagin (PL; 5-hydroxy-2-methyl-1, 4-naphthoquinone) is an important secondary metabolite, mainly produced in the Plumbago zeylanica L. (Plumbaginaceae). A single-chain variable fragment (scFv) antibody, fusion of the variable regions of the heavy chain and light chain of immunoglobulin against PL (PL-scFv) was expressed by Bac-to-Bac Baculovirus Expression System using Spodoptera frugiperda (Sf9) insect cells and characterized to investigate potential use of PL-scFv as a tool for plant immunomodulation. Functional PL-scFv expressed in the Sf9 insect cells were purified using cation exchange chromatography followed by immobilized metal ion affinity chromatography (IMAC). The yields of the purified PL-scFv in the culture supernatant and Sf9 insect cells were 2.0 mg and 5.2 mg per 1 liter of Sf9 culture medium, respectively. Recombinant purified PL-scFv was then characterized by the indirect competitive enzyme-linked immunosorbent assay (ELISA). The cross-reactivity and sensitivity of PL-scFv expressed in Sf9 insect cells were compared with PL-scFv expressed in Escherichia coli and its parental anti-plumbagin monoclonal antibody (MAb 3A3) secreted from hybridoma cells. Intriguingly, the specificity of the PL-scFv expressed in Sf9 insect cells was found to be different from that expressed in E. coli and parental MAb 3A3, although the detectable level (0.2-25 μg/mL) was the same in ELISA using each antibody. Even more interestingly, the characteristics of PL-scFv, which have wide cross-reactivity against 1,4-napththoquinone, suggest its potential use as a tool for plant immunomodulation not only for breeding Plumbaginacea family containing PL but also for breeding other medicinal plants containing bioactive naphthoquinones.Hybridoma (2005) 11/2010; 29(6):481-8. · 0.42 Impact Factor
Article: An efficient regeneration and plantlet development protocol from somatic tissues of Plumbago zeylanica L[show abstract] [hide abstract]
ABSTRACT: A rapid clonal multiplication protocol comprising direct multiple shoot induction from nodal explants of Plumbago zeylanica L was developed. Explants were cultured on standard Murashige and Skoog (MS) medium supplemented with different concentrations of benzyl amino purine (BAP) or kinetin (KIN) or in combinations with different concentrations of IBA, IAA, TIBA, coconut milk, adenine sulfate or activated charcoal. The best shoot proliferation (41.77 shoots per explants with 100% induction was observed in MS medium containing 1.5 mgl-1 BAP +0.75 mgl-1 IBA + 0.75 mgl-1 AdS + 10% CM. For rooting of microshoots, half strength MS medium supplemented with 0.75 mgl-1 IBA showed best results with 19.46 roots per shoot at an average root length of 7.03 cm with average rooting response of 99.67%. After acclimatization and transplantation, 100% of the In- vitro derived plants were found healthy in ex vivo conditions.Journal of Pharmacy Research. 01/2011; 4:2860-2863.