[Study on animal models for hyperlipidemia].

Department of Nutrition and Food Hygiene, Beijing University, Beijing 100083, China.
Wei sheng yan jiu = Journal of hygiene research 05/2002; 31(2):97-9.
Source: PubMed

ABSTRACT To establish a better animal model for hyperlipidemia, three animal models with SD rats, Wistar rats and golden hamsters were explored. The semi-purified diet for rats was composed of 1% cholesterol, 10% lard, 10% yolk powder and 79% normal diet. The semi-purified diet for hamster was composed of 0.2% cholesterol, 15% lard and 85% normal diet. Serum TC, TG, HDL-C, liver cholesterol and triglyceride were assayed. Serum TG of SD rat, Wistar rat and hamster was 194.4%, 86.2% and 84.3% respectively higher than that of control after 5 weeks of experiment. Serum TC of the three groups was 76.8%, 48.3% and 134.8% respectively higher than that of control. The results suggested that hamster might be a better animal model for hypercholesterolemia and SD rat might be better for hypertriglyceridemia.

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    ABSTRACT: The objective of this work was to establish a novel Mongolian gerbil (Meriones unguiculatus) hyperlipidemia model and to investigate its susceptibility genetic basis. Two rodent (gerbil and rat) hyperlipidemia models were induced by feeding a high fat/high-cholesterol (HF/HC) diet. There were significant increases of serum total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in gerbils within a 4-week modeling period. About 10-30% of >8-month-old individuals developed hyperlipidemia spontaneously. The apolipoprotein E (ApoE) gene was cloned by merging a sequence of rapid amplification of cDNA ends (RACE) and nested polymerase chain reaction products. The results revealed an open reading frame of 948 bp, encoding a protein of 298 amino acids. The gene without a 5'-UTR region in the first intron was highly homologous to human Apo-A-I and rat Apo-A-IV. The distribution of expression of the ApoE gene in liver, brain, heart, lung, kidney, and adrenal gland was detected by an ABC immunohistochemical procedure. Three single nucleotide polymorphisms (SNPs; C97T, G781T, and A1774T) were first found using PCR-single-strand conformation polymorphism (PCR-SSCP) in a closed population containing 444 animals. Correlation analysis confirmed that new SNPs , age, and gender were associated significantly (P < 0.05) with hyperlipidemia.
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