Leukemia inhibitory factor is a key signal for injury-induced neurogenesis in the adult mouse olfactory epithelium

Unité Mixte de Recherche 5020 Centre National de la Recherche Scientifique, Université Lyon I, 69622 Villeurbanne, France.
The Journal of Neuroscience : The Official Journal of the Society for Neuroscience (Impact Factor: 6.34). 04/2003; 23(5):1792-803.
Source: PubMed


The mammalian olfactory epithelium (OE) is composed of primary olfactory sensory neurons (OSNs) that are renewed throughout adulthood by local, restricted neuronal progenitor cells. The molecular signals that control this neurogenesis in vivo are unknown. Using olfactory bulb ablation (OBX) in adult mice to trigger synchronous mitotic stimulation of neuronal progenitors in the OE, we show the in vivo involvement of a cytokine in the cellular events leading to the regeneration of the OE. We find that, of many potential mitogenic signals, only leukemia inhibitory factor (LIF) is induced before the onset of neuronal progenitor proliferation. The rise in LIF mRNA expression peaks at 8 hr after OBX, and in situ RT-PCR and immunocytochemistry indicate that LIF is upregulated, in part, in the injured neurons themselves. This rise in LIF is necessary for injury-induced neurogenesis, as OBX in the LIF knock-out mouse fails to stimulate cell proliferation in the OE. Moreover, delivery of exogenous LIF to the intact adult OE using an adenoviral vector stimulates BrdU labeling in the apical OE. Taken together, these results suggest that injured OSNs release LIF as a stimulus to initiate their own replacement.

Download full-text


Available from: Emmanuel Moyse, Mar 12, 2014
  • Source
    • "The mechanism of this new interaction has not been explored yet, but in any case the results are consistent with findings from various in vivo studies suggesting that a 2 -adrenergic antagonists may have neuroprotective properties, both in the absence and presence of opioids. Thus, dexefaroxan increases cell survival in the olfactory bulb and dentate gyrus of the hippocampus of rats (Bauer et al., 2003; Rizk et al., 2006), and yohimbine itself was found to prevent reactive astrogliosis provoked by chronic morphine treatment in the rat brain (Alonso et al., 2007; Garrido et al., 2005). The present study extends the number of interactions already described between opioid and a 2 -adrenoceptor ligands and further supports the idea that opioid neurotoxicity can be negatively modulated by concomitant exposure to a 2 adrenoceptor antagonists, as previously described with analgesia and addiction. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Short-term incubation with pharmacologically relevant concentrations of morphine has been shown to transiently affect the metabolism and redox status of NG108-15 cells through δ-opioid receptor stimulation, but apparently did not provoke cell death. The present work tries to determine if incubation with morphine at longer time intervals (24 h) provokes apoptosis and/or necrosis, as it has been described in other cell lines. We have also checked the potential modulatory role of yohimbine on these effects, on the basis of the previously described interactions between this drug and opioid receptor ligands. Incubation with morphine 0.1 and 10 μM provoked the appearance of images compatible with apoptosis (bebbling, pyknotic cells with cytoplasmic and nuclear condensation) and necrosis (cells swollen with vacuolated cytoplasm lacking cell processes) that could be observed directly and/or after staining with methylene blue, crystal violet and propidium iodide/4',6-diamidino-2-phenylindole (IP/DAPI). Quantification of apoptosis by activation of caspases 3 and 7 and DNA fragmentation with the Tunel assay revealed a modest but significant increase after incubation with the two concentrations of morphine used. Co-incubation with 10 μM yohimbine prevented all these effects of the opioid. The results extend previous findings of a yohimbine-sensitive, neurotoxic effect of morphine on NG108-15 cells. Copyright © 2012 John Wiley & Sons, Ltd.
    Journal of Applied Toxicology 03/2014; 34(1). DOI:10.1002/jat.2817 · 2.98 Impact Factor
  • Source
    • "Consistent with the conclusion that damage drives OSN replacement, the proliferation of new OSNs is accelerated by damage and slowed by protective manipulations [6,7], events that are controlled by local signals impinging on the progenitor cells [8-16]. Analogous to the transition of embryonic neuroepithelial cells into astroglial-like adult neural stem cells located in the subventricular zone of the brain [17], these local progenitors derive from embryonic neuroepithelial cells that seed a layer, several cells thick, of basal cells located just above the basal lamina of the olfactory epithelium. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Adult neurogenesis, fundamental for cellular homeostasis in the mammalian olfactory epithelium, requires major shifts in gene expression to produce mature olfactory sensory neurons (OSNs) from multipotent progenitor cells. To understand these dynamic events requires identifying not only the genes involved but also the cell types that express each gene. Only then can the interrelationships of the encoded proteins reveal the sequences of molecular events that control the plasticity of the adult olfactory epithelium. Of 4,057 differentially abundant mRNAs at 5 days after lesion-induced OSN replacement in adult mice, 2,334 were decreased mRNAs expressed by mature OSNs. Of the 1,723 increased mRNAs, many were expressed by cell types other than OSNs and encoded proteins involved in cell proliferation and transcriptional regulation, consistent with increased basal cell proliferation. Others encoded fatty acid metabolism and lysosomal proteins expressed by infiltrating macrophages that help scavenge debris from the apoptosis of mature OSNs. The mRNAs of immature OSNs behaved dichotomously, increasing if they supported early events in OSN differentiation (axon initiation, vesicular trafficking, cytoskeletal organization and focal adhesions) but decreasing if they supported homeostatic processes that carry over into mature OSNs (energy production, axon maintenance and protein catabolism). The complexity of shifts in gene expression responsible for converting basal cells into neurons was evident in the increased abundance of 203 transcriptional regulators expressed by basal cells and immature OSNs. Many of the molecular changes evoked during adult neurogenesis can now be ascribed to specific cellular events in the OSN cell lineage, thereby defining new stages in the development of these neurons. Most notably, the patterns of gene expression in immature OSNs changed in a characteristic fashion as these neurons differentiated. Initial patterns were consistent with the transition into a neuronal morphology (neuritogenesis) and later patterns with neuronal homeostasis. Overall, gene expression patterns during adult olfactory neurogenesis showed substantial similarity to those of embryonic brain.
    Molecular Brain 11/2013; 6(1):49. DOI:10.1186/1756-6606-6-49 · 4.90 Impact Factor
  • Source
    • "This phenomenon could be due to a reduction in the survival of basal cells and immature olfactory sensory neurons or the upregulation of either NPY Y1 receptors on basal cells or positive regulators of neurogenesis to compensate for the decrease in basal cells. Possibilities of positive regulators include fibroblast growth factor and transforming growth factor [40], amidated peptides [41], leukemia inhibitory factor [42] and OMP [43]. The overall implications of these results are that an appropriate population of neural stem cells is maintained by IP3R3+ microvillous cells by the production and release of trophic factor NPY. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Calcium-dependent release of neurotrophic factors plays an important role in the maintenance of neurons, yet the release mechanisms are understudied. The inositol triphosphate (IP3) receptor is a calcium release channel that has a physiological role in cell growth, development, sensory perception, neuronal signaling and secretion. In the olfactory system, the IP3 receptor subtype 3 (IP3R3) is expressed exclusively in a microvillous cell subtype that is the predominant cell expressing neurotrophic factor neuropeptide Y (NPY). We hypothesized that IP3R3-expressing microvillous cells secrete sufficient NPY needed for both the continual maintenance of the neuronal population and for neuroregeneration following injury. We addressed this question by assessing the release of NPY and the regenerative capabilities of wild type, IP3R3(+/-), and IP3R3(-/-) mice. Injury, simulated using extracellular ATP, induced IP3 receptor-mediated NPY release in wild-type mice. ATP-evoked NPY release was impaired in IP3R3(-/-) mice, suggesting that IP3R3 contributes to NPY release following injury. Under normal physiological conditions, both IP3R3(-/-) mice and explants from these mice had fewer progenitor cells that proliferate and differentiate into immature neurons. Although the number of mature neurons and the in vivo rate of proliferation were not altered, the proliferative response to the olfactotoxicant satratoxin G and olfactory bulb ablation injury was compromised in the olfactory epithelium of IP3R3(-/-) mice. The reductions in both NPY release and number of progenitor cells in IP3R3(-/-) mice point to a role of the IP3R3 in tissue homeostasis and neuroregeneration. Collectively, these data suggest that IP3R3 expressing microvillous cells are actively responsive to injury and promote recovery.
    PLoS ONE 03/2013; 8(3):e58668. DOI:10.1371/journal.pone.0058668 · 3.23 Impact Factor
Show more