A study of the morphological parameters of cervical squamous epithelium

Medical Physics and Engineering, University of Sheffield, Floor I, Royal Hallamshire Hospital, Glossop Road, Sheffield S 10 2JF, UK.
Physiological Measurement (Impact Factor: 1.81). 03/2003; 24(1):121-35. DOI: 10.1088/0967-3334/24/1/309
Source: PubMed


Electrical impedance spectroscopy is a technique that has been investigated as a potential method for the diagnosis of epithelial carcinomas. Finite element modelling can provide an insight into the patterns of current flow in normal and pathological epithelium and hence aid in the process of probe design optimization. In order to develop a finite element model of the structure of normal and precancerous cervical squamous epithelium, it was first necessary to obtain the mean values and ranges of a number of morphological tissue parameters. The most important parameters in discriminating normal from neoplastic tissue were identified as being cell size and shape distribution, nuclear-to-cytoplasmic volume ratio and volume of extracellular space. A survey of the literature revealed an absence of reliable quantitative data for these parameters. We therefore present the results of our own basic image analysis on normal and pathological tissue sections, which we hope will be of use to other workers wishing to model cervical squamous epithelium, or other similar tissue structures.

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    • "y Figure S2 ) . To quantify morphological proper - ties , surface areas of both whole cells and nuclei were deter - mined . They served as parameters to calculate the N / C ratio ( i . e . a nucleus surface area divided by a cytoplasm surface area ) that might be used as a marker to diagnose melanoma similarly to the cervical squamous epithelium ( Walker et al . 2003 ) . Melanoma cells cultured at higher density have Fig . 3 Correlated topography AFM imaging and epifluorescence microscopy obtained for a – e WM115 and a′ – e′ WM266 - 4 cells . Scale bars ( a , b and a′ , b′ ) 20 µm , ( c – e and c′ – e′ ) 5 µm . The F - actin was stained with rhodamine - phalloidin displayed occasional partial overla"
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