Structural heterogeneity and genomic distribution of Drosophila melanogaster LTR-retrotransposons.

Area de Genética, Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, Spain.
Molecular Biology and Evolution (Impact Factor: 14.31). 04/2003; 20(3):401-9.
Source: PubMed

ABSTRACT Structural heterogeneity of five long terminal repeat (LTR) retrotransposon families (297, mdg 1, 412, copia, and 1731) was investigated in Drosophila melanogaster. The genomic distribution of canonical and rearranged elements was studied by comparing hybridization patterns of Southern blots on salivary glands from adult females and males with in situ hybridization on polytene chromosomes. The proportion and genomic distribution of noncanonical copies is distinctive to each family and presents constant features in the four different D. melanogaster strains studied. Most elements of families 297 and mdg 1 were noncanonical and presented large interstock and intrastock polymorphism. Noncanonical elements of these two families were mostly located in euchromatin, although not restricted to it. The elements of families 412 and copia were better conserved. The proportion of noncanonical elements was lower. The 1731 family is mainly composed of noncanonical, beta-heterochromatic elements that are highly conserved among stocks. The relation of structural polymorphism to phylogeny, transpositional activity and the role of natural selection in the maintenance of transposable elements are discussed.

  • Doklady Biochemistry and Biophysics 05/2012; 444:171-4. · 0.37 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Mobile elements (MEs) are an important component of the genome in all eukaryotes and prokaryotes. MEs are divided into two large classes differing in the mechanism of transposition. Class I MEs are transposed via reverse transcription of their RNA transcripts. Class II MEs code for transposase, which acts at the DNA level and recognizes the ends of the cognate ME. The review considers the distribution of MEs from different classes in various genomes, individual chromosomes, and chromatin types. There is ample evidence for an important role of MEs in the regulation of cell genes and evolution of complex eukaryotic genomes. It is thought that ME invasion and subsequent amplification act as a main morphogenetic factor ensuring adaptation of populations to environmental changes and, in some cases, cause rapid speciation.
    Molecular Biology 03/2007; 41(2):203-213. · 0.74 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The role of transposable elements as controlling and destabilizing factors of the genome of germ and somatic cells is considered. The ability of various stress factors, both intragenomic (hybrid dysgenesis, inbreeding, and outbreeding) and environmental (heat and ionizing radiation), to induce transposable element transposition is analyzed.
    Russian Journal of Genetics: Applied Research. 12/2011; 1(6).


Available from
Jul 9, 2014