Expression of perivitelline membrane glycoprotein ZP1 in the liver of Japanese quail (Coturnix japonica) after in vivo treatment with diethylstilbestrol.
ABSTRACT Avian perivitelline membrane, an oocyte extracellular matrix homologous to the zona pellucida in mammals or chorion in fish, is composed of at least two glycoproteins. Previous studies have indicated that one of the components, a glycoprotein homologous to mammalian ZPC, is produced in the granulosa cells of the developing follicles of quail ovary on stimulation with testosterone. However, little is known about the molecular biology of the other component of the avian perivitelline membrane, ZP1, and information about gene expression is particularly lacking. We have cloned the ZP1 in Japanese quail and examined its gene expression. A cDNA encoding quail ZP1 was isolated from the livers of mature females using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. It encoded a 934-amino acid protein that showed greatest homology (87.8% identity) with the chicken ZP1. RT-PCR amplification indicated that the ZP1 mRNA in the liver was restricted to mature laying females. The expression of ZP1 mRNA was stimulated by in vivo treatment with diethylstilbestrol in immature females as well as males. These results suggested that androgens and estrogens coordinately regulate the formation of quail perivitelline membrane proteins. In addition, the use of ZP1 transcriptional induction in males or immature females as a biological marker of environmental estrogens is discussed.
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ABSTRACT: Estrogenic chemicals have drawn considerable attention over the past several years because they are known to be responsible for increased breast and prostate cancer. Vitellogenin expression driven by estrogen has been becoming a model for studying estrogenic effects in aquatic species. For the first time, we showed evidence that, without stimulation, vitellogenin mRNA precursor is expressed in both male and female immature fish. After 4 h, in fish treated with estradiol, the vitellogenin mRNA was synthesized and the precursor mRNA began to disappear. The environmental chemical, nonylphenol, showed the same effect on the vitellogenin gene expression as estrogen. It is suggested that estrogen and nonylphenol may be involved in a post-transcriptional regulation process -- possibly in the initiation of pre-mRNA splicing.Chemico-Biological Interactions 04/1996; 100(1):67-76. · 2.97 Impact Factor
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ABSTRACT: A substance with high immunoreactivity to rabbit anti-chorion (egg envelope) glycoprotein antibody was found in liver and blood plasma as well as ovary of the spawning female medaka, while it was absent in the male and the non-spawning female fish. This substance disappeared first from the hepatic cells of the female fish when it ceased spawning, whereas the immunoreactivity still remained in ovary and blood plasma. The relative electrophoretic mobility of the antigenic substance derived from these tissues was identical. The molecular weight and isoelectric point (pI) of the substance, as determined by polyacrylamide electrophoresis with varied gel concentrations and isoelectric focusing method, were about 60,000 and about 4.5, respectively. The substance was evidently distinct from the unreactive band of probable vitellogenin (ca 420,000 and pI, ca 3.8–4.0) which was found in blood plasma of the spawning female. In ovary sections, cytoplasm of the oocyte at the initial stage of chorion formation, containing neither vitellogenin nor egg yolk, showed a strong immunoreactivity. Cytoplasm of the oocytes, later than the vitellogenic stage, exhibited little immunoreactivity. Changes of this substance in connection with the spawning cycle strongly suggest that this substance is related to oogenesis.Journal of Experimental Zoology 04/2005; 235(2):269 - 279.
- Biology of Reproduction 10/1980; 23(2):386-93. · 4.03 Impact Factor