In vitro cytocompatibility studies of Diamond Like Carbon coatings on titanium.

T V Kumari, P R Anil Kumar, C V Muraleedharan, G S Bhuvaneshwar, Y Sampeur, F Derangere, R Suryanarayanan

BMT Wing, Sree Chitra Tirunal Institute of Medical Sciences & Technology, Poojapura, Thiruvananthapuram, Kerala 695 012, India.

Journal Article: Bio-medical materials and engineering (impact factor: 0.62). 02/2002; 12(4):329-38.

Abstract

Diamond like carbon (DLC) films were deposited on to titanium (Ti) substrates by Plasma Enhanced Chemical Vapour Deposition (PECVD) process. The quality of the films were checked by Raman spectra and nano-hardness tests. The cytocompatibility of titanium and DLC coated titanium were studied using continuous cell lines of mouse fibroblast cells ( L-929), Human Osteoblast cells (HOS) and primary human umbilical cord vein endothelial cells (HUVEC). The cellular responses to the materials were assessed both quantitatively and qualitatively. The adhesion and spreading of cells on materials were compared using Ti as a control. Present study indicates an improved cytocompatibility of DLC coated Ti in comparison to bare Ti.

Source: PubMed

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Keywords

bare Ti
 
cellular responses
 
continuous cell lines
 
DLC
 
Human Osteoblast cells
 
mouse fibroblast cells
 
nano-hardness tests
 
Plasma Enhanced Chemical Vapour Deposition
 
primary human umbilical cord vein endothelial cells
 
quantitatively
 
Raman spectra
 
titanium