Shift of whispering-gallery modes in microspheres by protein adsorption
ABSTRACT Biosensors based on the shift of whispering-gallery modes in microspheres accompanying protein adsorption are described by use of a perturbation theory. For random spatial adsorption, theory predicts that the shift should be inversely proportional to microsphere radius R and proportional to protein surface density and excess polarizability. Measurements are found to be consistent with the theory, and the correspondence enables the average surface area occupied by a single protein to be estimated. These results are consistent with crystallographic data for bovine serum albumin. The theoretical shift for adsorption of a single protein is found to be extremely sensitive to the target region, with adsorption in the most sensitive region varying as 1/R(5/2). Specific parameters for single protein or virus particle detection are predicted.
SourceAvailable from: Christopher Courtney Evans[Show abstract] [Hide abstract]
ABSTRACT: Third-harmonic generation (THG) has applications ranging from wavelength conversion to pulse characterization, and has important implications for quantum sources of entangled photons. However, on-chip THG devices are nearly unexplored because bulk techniques are difficult to adapt to integrated photonic circuits. Using sub-micrometer-wide polycrystalline anatase TiO<sub>2</sub> waveguides, we demonstrate third-harmonic generation on a CMOS-compatible platform. We correlate higher conversion efficiencies with phase-matching between the fundamental pump mode and higher-order signal modes. Using scattered light, we estimate conversion efficiencies as high as 2.5% using femtosecond pulses, and thus demonstrate that multimode TiO<sub>2</sub> waveguides are promising for wideband wavelength conversion and new applications ranging from sensors to triplet-photon sources.Optics Express 03/2015; 23(6):7832-41. · 3.53 Impact Factor
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ABSTRACT: We report narrow linewidth optomechanical oscillation of a silica microsphere immersed in a buffer solution. Through a novel optomechanical transduction sensing approach, single 10-nm-radius silica beads and Bovine serum albumin (BSA) protein molecules with a molecular weight of 66 kDalton were detected. This approach predicts the detection of 3.9 kDalton single molecules at a signal-to-noise ration above unity.
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ABSTRACT: Whispering-gallery modes have been studied extensively for biosensing applications. Whilst the vast majority of work undertaken has focused on high Q factor resonators, with the main improvement being a reduction of the resonator size to improve sensitivity, we have chosen a different pathway by starting with resonators that exhibit extremely high refractive index sensitivity but low Q factor. A way forward to overcome this limitation is to introduce a gain medium and operate the resonator above its lasing threshold. This has been shown to result on average in a 5 fold increase in the Q factor. With the lasing threshold itself being dependent on the Q factor, amongst other parameters, the Q factor enhancement can be exploited to either reduce the lasing threshold or alternatively enable smaller resonators to be operated above their lasing threshold. As a demonstration we present a 10 μm diameter polystyrene microsphere lasing in aqueous solution for refractive index sensing applications, which to the best of our knowledge is the smallest polystyrene microsphere laser ever demonstrated in these conditions.Laser Resonators, Microresonators, and Beam Control XVII, San Francisco, California, United States; 02/2015