The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland.

Department of Microbiology and Parasitology, College of Veterinary Medicine and Animal Resources, King Faisal University, PO Box 35252, Al-AHSAA, 31982 Saudi Arabia.
Journal of Veterinary Medicine Series B (Impact Factor: 1.48). 05/2003; 50(3):105-11. DOI: 10.1046/j.1439-0450.2003.00628.x
Source: PubMed

ABSTRACT TaqMan real time PCR was used to study the transcriptional activity of the bovine IL-2, IL-6, IL-12p40, IFN-gamma, TNF-alpha and granulocyte-monocyte colony stimulating factor of whole milk cells in bovine mammary gland experimentally infected with Staphylococcus aureus. Cytokine transcriptional activity was monitored at 7, 24 and 32 h Post-infection (Pi). IL-12 and TNF-alpha levels were significantly elevated at 24 h Pi followed by sharp decrease at 32 h pi. IL-2 level was decreased at 32 h pi. IL-12 and IFN-gamma showed a significant interaction at 24 h pi. The significant elevations of the IL-12 and TNF-alpha transcriptional level most likely indicate their important role in regulation of the immune responses of bovine mammary gland in S. aureus infection. Depression of IL-2 could reflect the suppressive nature of the S. aureus mastitis.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Staphylococcus aureus is the pathogen most frequently isolated from bovine mastitis worldwide, causing chronic intramammary infections that limit profitable dairying. The objective of this article is to characterize the mechanisms involved in S. aureus mammary gland infections considering two different aspects of the infectious process; on the one hand, the aspects involved in the host innate immune response and on the other hand, the capacity of this organism to evade the immune system and interact with different cell types. The exploration of S. aureus interactions with the immune response of bovine mammary gland will help identify targets to outline new preventive or curative alternatives for intramammary infections caused by this organism.
    Revista Argentina de Microbiología. 12/2014; 16.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Mastitis is one of the major diseases of dairy animals. Staphylococcus aureus (S. aureus) is among the most important aetiological agents which cause mastitis. As we know, there were no reports on the changes of mineral metabolism, cytokines and cortisol in S. aureus induced mastitis. In the present study, fifteen dairy goats were used to determine whether concentrations of serum minerals, cytokines and cortisol differ following immune activation with low dose (LDSA) and high dose of S. aureus (C56010, HDSA) intramammary inoculation. Serum iron concentrations were decreased (P < 0.05) in S. aureus challenged groups, however, serum copper, zinc and magnesium concentrations were increased. Plasma tumor necrosis factor-α (TNF-α) concentrations were unaffected throughout the study. Plasma interleukin-6 (IL-6) concentrations were increased in S. aureus challenged groups. In group HDSA, serum cortisol concentrations increased. In conclusion, intramammary inoculation with S. aureus showed a clear influence on mineral metabolism in the state of immune activation, and this may take place in the absence of significant changes in the systemic proinflammatory mediators.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Staphylococcus aureus is a major mastitis-causing pathogen in dairy cows. The latex agglutination-based Staphaurex test allows bovine S. aureus strains to be grouped into Staphaurex latex agglutination test (SLAT)-negative [SLAT(-)] and SLAT-positive [SLAT(+)] isolates. Virulence and resistance gene profiles within SLAT(-) isolates are highly similar, but differ largely from those of SLAT(+) isolates. Notably, specific genetic changes in important virulence factors were detected in SLAT(-) isolates. Based on the molecular data, it is assumed that SLAT(+) strains are more virulent than SLAT(-) strains. The objective of this study was to investigate if SLAT(-) and SLAT(+) strains can differentially induce an immune response with regard to their adhesive capacity to epithelial cells in the mammary gland and in turn, could play a role in the course of mastitis. Primary bovine mammary epithelial cells (bMEC) were challenged with suspensions of heat inactivated SLAT(+) (n = 3) and SLAT(-) (n = 3) strains isolated from clinical bovine mastitis cases. After 1, 6, and 24 h, cells were harvested and mRNA expression of inflammatory mediators (TNF-α, IL-1β, IL-8, RANTES, SAA, lactoferrin, GM-CSF, COX-2, and TLR-2) was evaluated by reverse transcription and quantitative PCR. Transcription (ΔΔCT) of most measured factors was induced in challenged bMEC for 6 and 24 h. Interestingly, relative mRNA levels were higher (P<0.05) in response to SLAT(+) compared to SLAT(-) strains. In addition, adhesion assays on bMEC also showed significant differences between SLAT(+) and SLAT(-) strains. The present study clearly shows that these two S. aureus strain types cause a differential immune response of bMEC and exhibit differences in their adhesion capacity in vitro. This could reflect differences in the severity of mastitis that the different strain types may induce.
    PLoS ONE 01/2014; 9(1):e87374. · 3.53 Impact Factor

Full-text (2 Sources)

Available from
Aug 7, 2014