Article

The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland.

Department of Microbiology and Parasitology, College of Veterinary Medicine and Animal Resources, King Faisal University, PO Box 35252, Al-AHSAA, 31982 Saudi Arabia.
Journal of Veterinary Medicine Series B (Impact Factor: 1.48). 05/2003; 50(3):105-11. DOI: 10.1046/j.1439-0450.2003.00628.x
Source: PubMed

ABSTRACT TaqMan real time PCR was used to study the transcriptional activity of the bovine IL-2, IL-6, IL-12p40, IFN-gamma, TNF-alpha and granulocyte-monocyte colony stimulating factor of whole milk cells in bovine mammary gland experimentally infected with Staphylococcus aureus. Cytokine transcriptional activity was monitored at 7, 24 and 32 h Post-infection (Pi). IL-12 and TNF-alpha levels were significantly elevated at 24 h Pi followed by sharp decrease at 32 h pi. IL-2 level was decreased at 32 h pi. IL-12 and IFN-gamma showed a significant interaction at 24 h pi. The significant elevations of the IL-12 and TNF-alpha transcriptional level most likely indicate their important role in regulation of the immune responses of bovine mammary gland in S. aureus infection. Depression of IL-2 could reflect the suppressive nature of the S. aureus mastitis.

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