Article
Evidence on inhibition of Listeria monocytogenes by divercin V41 action.
Laboratoire de Microbiologie Alimentaire et Industrielle, ENTIAA, Rue de la Géraudière, Nantes cedex, France.
Letters in Applied Microbiology (impact factor:
1.62).
02/2003;
36(5):288-92.
pp.288-92
Source: PubMed
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Citations (0)
- Cited In (2)
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Article: Heterologous expression and purification of active divercin V41, a class IIa bacteriocin encoded by a synthetic gene in Escherichia coli.
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ABSTRACT: Divercin V41, a class IIa bacteriocin with strong antilisterial activity, is produced by Carnobacterium divergens V41. To express a recombinant version of divercin V41, we constructed a synthetic gene that encodes the mature divercin V41 peptide and then overexpressed the gene in pET-32b by using the T7 RNA polymerase promoter in the Escherichia coli Origami (DE3)(pLysS) strain. The DvnRV41 peptide was expressed as a translational fusion protein with thioredoxin and accumulated in the cell cytoplasm in a soluble anti-Listeria active form. The fusion protein was then purified and cleaved to obtain pure, soluble, folded DvnRV41 (462 microg per 20 ml of culture). This paper describes the first design of a synthetic bacteriocin gene and the first bacteriocin expressed in the E. coli cytoplasm.Journal of Bacteriology 08/2004; 186(13):4276-84. · 3.83 Impact Factor -
Article: Generation and utilization of polyclonal antibodies to a synthetic C-terminal amino acid fragment of divercin V41, a class IIa bacteriocin.
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ABSTRACT: Polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized C-terminal part of divercin V41 (DvnCt) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and reactivity of the DvnCt-KLH-generated antibodies were evaluated by enzyme-linked immunosorbent assay (ELISA) using supernatant from cultures of 13 representative lactic acid bacterium strains, and specificity was confirmed by Western blot analysis. Anti-DvnCt-KLH antibodies were able to recognize not only divercin V41 but also enterocin P and piscicocin V1b, two other members of the class IIa bacteriocins. Production and activity of DvnV41 were evaluated by ELISA and activity tests during the growth of Carnobacterium divergens V41 in MRS medium containing or not containing Tween 80. Divercin V41, enterocin P, and piscicocin V1b were therefore purified by a single-step immunoaffinity chromatography method using a Sepharose matrix CNBr-activated directed binding of anti-DvnCt-KLH polyclonal antibodies.Applied and Environmental Microbiology 02/2004; 70(1):248-54. · 3.83 Impact Factor
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Keywords
area food protection
bacteriocin
bacteriocin diffusion test
bacteriocin production
C. divergens V41
C. divergens V41C9
Carnobacterium divergens V41
Carnobacterium divergens V41 deficient
Carnobacterium divergens V41C9
divercin V41
divercin+
enzyme-liked immunosorbent assay
food safety
inhibition
L. monocytogenes
lactic acid bacteria
Listeria monocytogenes
multiplex polymerase chain reaction
smoked salmon model medium
