Nitroxyl gets to the heart of the matter.

Louisiana State University Health Sciences Center, Department of Molecular and Cellular Physiology, 1501 Kings Highway, Shreveport, LA 71130, USA.
Proceedings of the National Academy of Sciences (Impact Factor: 9.81). 05/2003; 100(9):4978-80. DOI: 10.1073/pnas.1031571100
Source: PubMed
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    ABSTRACT: A fast-response, highly sensitive and selective fluorescent probe with the 2-(diphenylphosphino)benzoate moiety as a recognition receptor for the ratiometric imaging of nitroxyl in living cells was first developed.
    Chemical Communications 04/2014; 50(45). DOI:10.1039/c4cc00980k · 6.72 Impact Factor
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    ABSTRACT: It is generally established that the intracellular reactive nitrogen species (RNS) which contain nitrogen atoms are one class of highly chemical active species. These species have attracted increasing attention and become an active research field based on their key roles in special functions during a series of physiological and pathological processes. In order to elucidate these roles of RNS, the design and development technology for selective and sensitive detection to RNS in vivo are crucial. Advanced with high sensitivity, good selectivity, noninvasive detection and real-time visualization in situ, fluorescent probes provide facilitative and effective chemical approaches in modern biochemistry analysis. Progress in the field of fluorescent probes for RNS promises to advance our knowledge of essential cellular signal transduction during the varieties of physiological and pathological processes, which is indicated in human health and disease. According to the current situation, we review the past four years'latest five types of RNS probes for nitric oxide (NO), peroxynitrite (ONOO-), nitroxyl (HNO), nitrite (NO2-) and nitrogen dioxide (NO2). In this article, the design strategies, fluorescent response mechanisms and biological applications of the probes are discussed. Finally, the prospect to design and applications of probes is given.
    Progress in Chemistry -Beijing- 03/2014; 26(5):866-878. DOI:10.7536/PC131025 · 0.71 Impact Factor
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    ABSTRACT: HNO has broad biological effects and pharmacological activities. Direct HNO probes for in vivo applications were recently reported, which are Cu(II)-based complexes having fluorescence reporters with reaction to HNO resulting in Cu(I) systems and the release of NO. Their coordination environments are similar to that in Cu,Zn-superoxide dismutase (SOD), which plays a significant role in cellular HNO/NO conversion. However, none of these conversion mechanisms are known. A quantum chemical investigation was performed here to provide structural, energetic, and electronic profiles of HNO/NO conversion pathways via the first Cu(II)-based direct HNO probe. Results not only are consistent with experimental observations but also provide numerous structural and mechanistic details unknown before. Results also suggest the first HNO/NO conversion mechanism for Cu,Zn-SOD, as well as useful guidelines for future design of metal-based HNO probes. These results shall facilitate development of direct HNO probes and studies of HNO/NO conversions via metal complexes and metalloproteins.
    Journal of Physical Chemistry Letters 03/2014; 5(6):1022-1026. DOI:10.1021/jz5002902 · 6.69 Impact Factor


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