Leptospira in slaughtered fattening pigs in southern Vietnam: presence of the bacteria in the kidneys and association with morphological findings.
ABSTRACT One kidney was collected from each of 32 fattening pigs at an abattoir in southern Vietnam in 2001 in order to demonstrate infecting Leptospira serovar and to associate renal macro- and microscopic findings with the presence of renal leptospires. Leptospires were demonstrated in 22 (69%) of the investigated kidneys by immunofluorescence. Multifocal interstitial nephritis (MFIN) and gross renal lesions (white spots) were each demonstrated in 24 (75%) kidneys. Leptospira interrogans serovar bratislava was isolated from one kidney. There was no association between presence of leptospires and MFIN (P=0.19), respectively and white spots (P=0.98), respectively. These data suggest that Leptospira infection is common among fattening pigs in the study area and that these animals may be considered as an occupational human health hazard. It is also suggested that the presence of white spots is an unreliable indicator of the presence of renal leptospires.
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ABSTRACT: Las lesiones renales son una causa importante de decomiso en los mataderos. Además de las posibles consecuencias en salud pública, el decomiso de órganos tiene un gran impacto económico en la industria de alimento animal. Recientemente, nefritis embólica séptica con lesiones semejantes a infecciones con Actinobacillus equuli en potrillos ha sido detectada en reproductoras y cerdos con peso de mercado. Actinobacillus equuli es fenotípica y genéticamente similar a Actinobacillus suis. Ambas son bacterias Gram-negativas difíciles de diagnosticar en exámenes de rutina. A. suis es un patógeno oportunista capaz de producir septicemia en cerdos, neumonía, poliartritis, nefritis embólica séptica, aborto y fetos momificados. Brotes de la enfermedad clínica parecieran ocurrir con más frecuencia en planteles de cerdos con estrictas medidas de bioseguridad. En cerdos adultos, las lesiones de piel pueden confundirse con erisipela porcina. A. suis y A. equuli son patógenos oportunistas emergentes en la industria porcina y ambos tienen potenciales consecuencias en salud pública, principalmente en aquellas personas que manipulan productos cárneos. El objetivo de esta publicación es presentar una revisión bibliográfica sobre el rol de A. suis y A. equuli en la patogénesis de nefritis en cerdos.Archivos de Medicina Veterinaria 12/2011; 44(2):99-107. · 0.41 Impact Factor
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ABSTRACT: Leptospirosis is a worldwide zoonotic infection. The source of infection in humans is usually either direct or indirect contact with the urine of infected animals. Occupation is a significant risk factor for humans. Direct contact with infected animals accounts for most infections in farmers, veterinarians, abattoir workers, meat inspectors. The highest risk is associated with swine farming, slaughterhouse and meat industry workers. Most cases are diagnosed by serology. Leptospirosis may be prevented trought appropriate hygiene, sanitization, animal husbandry. It is essential to educate people working with animals or animal tissues about measures for reducing the risk of exposure to Leptospira. Protective clothing provided included an apron, gloves and rubber boots. When the risk is high, serologic testing are useful also.
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ABSTRACT: Leptospirosis is a growing public and veterinary health concern caused by pathogenic species of Leptospira. Rapid and reliable laboratory tests for the direct detection of leptospiral infections in animals are in high demand not only to improve diagnosis but also for understanding the epidemiology of the disease. In this work we describe a novel and simple TaqMan-based multi-gene targeted real-time PCR approach able to detect and differentiate Leptospira interrogans, L. kirschneri, L. borgpeteresenii and L. noguchii, which constitute the veterinary most relevant pathogenic species of Leptospira. The method uses sets of species-specific probes, and respective flanking primers, designed from ompL1 and secY gene sequences. To monitor the presence of inhibitors, a duplex amplification assay targeting both the mammal β-actin and the leptospiral lipL32 genes was implemented. The analytical sensitivity of all primer and probe sets was estimated to be <10 genome equivalents (GE) in the reaction mixture. Application of the amplification reactions on genomic DNA from a variety of pathogenic and non-pathogenic Leptospira strains and other non-related bacteria revealed a 100% analytical specificity. Additionally, pathogenic leptospires were successfully detected in five out of 29 tissue samples from animals (Mus spp., Rattus spp., Dolichotis patagonum and Sus domesticus). Two samples were infected with L. borgpetersenii, two with L. interrogans and one with L. kirschneri. The possibility to detect and identify these pathogenic agents to the species level in domestic and wildlife animals reinforces the diagnostic information and will enhance our understanding of the epidemiology of leptopirosis.PLoS ONE 11/2014; 9(11):e112312. · 3.53 Impact Factor