Dennis, G. et al. DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 4, R60

Science Applications International Corporation-Frederick, Clinical Services Program, Laboratory of Immunopathogenesis and Bioinformatics, National Cancer Institute at Frederick, MD 21702, USA.
Genome biology (Impact Factor: 10.81). 02/2003; 4(5):P3. DOI: 10.1186/gb-2003-4-9-r60
Source: PubMed


Functional annotation of differentially expressed genes is a necessary and critical step in the analysis of microarray data. The distributed nature of biological knowledge frequently requires researchers to navigate through numerous web-accessible databases gathering information one gene at a time. A more judicious approach is to provide query-based access to an integrated database that disseminates biologically rich information across large datasets and displays graphic summaries of functional information.
Database for Annotation, Visualization, and Integrated Discovery (DAVID; addresses this need via four web-based analysis modules: 1) Annotation Tool - rapidly appends descriptive data from several public databases to lists of genes; 2) GoCharts - assigns genes to Gene Ontology functional categories based on user selected classifications and term specificity level; 3) KeggCharts - assigns genes to KEGG metabolic processes and enables users to view genes in the context of biochemical pathway maps; and 4) DomainCharts - groups genes according to PFAM conserved protein domains.
Analysis results and graphical displays remain dynamically linked to primary data and external data repositories, thereby furnishing in-depth as well as broad-based data coverage. The functionality provided by DAVID accelerates the analysis of genome-scale datasets by facilitating the transition from data collection to biological meaning.

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    • "Ontologies enrichments related to BPA05 and BPA50 DEG were obtained by querying corresponding Entrez Gene IDs into the Database for Annotation, Visualization and Integrated Discovery (DAVID) (Dennis et al., 2003) and clustered for redundancy by the Functional Annotation Clustering of Gene Ontology (GO) terms so that each significant cluster included parent and children GO terms. Similarly, enrichments for KEGG pathways were obtained. "
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    ABSTRACT: Bisphenol A (BPA) is a widespread endocrine disrupter mainly used in food contact plastics. Much evidence supports the adverse effects of BPA, particularly on susceptible groups such as pregnant women. The present study considered placental development - relevant for pregnancy outcomes and fetal nutrition/programming - as a potential target of BPA. Pregnant CD-1 mice were administered per os with vehicle, 0.5 (BPA05) or 50 mg kg(-1) (BPA50) body weight day(-1) of BPA, from gestational day (GD) 1 to GD11. At GD12, BPA50 induced significant degeneration and necrosis of giant cells, increased vacuolization in the junctional zone in the absence of glycogen accumulation and reduction of the spongiotrophoblast layer. In addition, BPA05 induced glycogen depletion as well as significant nuclear accumulation of β-catenin in trophoblasts of labyrinthine and spongiotrophoblast layers, supporting the activation of the Wnt/β-catenin pathway. Transcriptomic analysis indicated that BPA05 promoted and BPA50 inhibited blood vessel development and branching; morphologically, maternal vessels were narrower in BPA05 placentas, whereas embryonic and maternal vessels were irregularly dilated in the labyrinth of BPA50 placentas. Quantitative polymerase chain reaction evidenced an estrogen receptor β induction by BPA50, which did not correspond to downstream genes activation; indeed, the transcription factor binding sites analysis supported the AhR/Arnt complex as regulator of BPA50-modulated genes. Conversely, Creb appeared as the main transcription factor regulating BPA05-modulated genes. Embryonic structures (head, forelimb) showed divergent perturbations upon BPA05 or BPA50 exposure, potentially related to unbalanced embryonic nutrition and/or to modulation of genes involved in embryo development. Our findings support placenta as an important target of BPA, even at environmentally relevant dose levels. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.
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    • "Kyoto Encyclopedia of Genes and Genomes ( KEGG Orthology ) by Database for Annotation , Visualization , and Integrated Discovery – DAVID Bioinformatics Resources 6 . 7® ( Dennis et al . , 2003"
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    • "In our experiment, the TFs, genes and miRNAs are then ranked with respect to eigenvector centrality (i.e., EC). Thereafter, we have considered each of the four gene-sets (viz., (DE down \ DM hyper ), (DE up \ DM hypo ), (DE up \ DM hyper ) and (DE down \ DM hypo )), separately for biological validation through KEGG pathways and GENE ONTOLOGY ENRICHMENT using the David database [54] that follows modified Fisher's Exact test (modified as EASE score) [56] "
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