Article

Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography.

Peking University Modern Research Center for Traditional Chinese Medicine, Beijing 100083, China.
Journal of Chromatography A (Impact Factor: 4.61). 05/2003; 992(1-2):193-7. DOI: 10.1016/S0021-9673(03)00315-7
Source: PubMed

ABSTRACT Two isoflavones, calycosin-7-O-beta-D-glycoside and formononetin-7-O-beta-D-glycoside, were separated from n-butanol extract of the root of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography in two steps using two different solvent systems composed of ethyl acetate-ethanol-n-butanol-water (30:10:6:50, v/v) and ethyl acetate-ethanol-water (5:1:5, v/v). From 200 mg of crude extract, calycosin-7-O-beta-D-glycoside (12 mg) and formononetin-7-O-beta-D-glycoside (10 mg) were isolated at over 95% purity by HPLC analyses, and their structures were identified by MS, 1H NMR and 13C NMR.

0 Bookmarks
 · 
73 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Phytochemical study of flowering shoots of Caragana arborescens Lam. (Fabaceae family) collected in Buryatia Republic (Russian Federation) resulted in the isolation of twenty compounds identified as quercetin, isoquercitrin and rutin, previously identified in this species, and β-sitosterol, β-sitosterol-3-O-glucoside, umbelliferone, kaempferol, formononetin, isorhamnetin-3-O-glucoside, nicotiflorin, narcissin, myricetin-3-O-rutinoside, ononin, formononetin-7-O-rutinoside, caffeic, 3-O-caffeoylquinic, 5-O-caffeoylquinic, 3,5-di-O-caffeoylquinic acids and sucrose, isolated for the first time from C. arborescens.
    Natural product communications 05/2013; 8(5):585-586. · 0.96 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Four chromones, prim-O-glucosylcimifugin, 4'-O-β-D-glucosyl-5-O-methylvisamminol, cimifugin and sec-O-glucosylhamaudol, were isolated and purified from Saposhnikovia divaricata for the first time by high-performance counter-current chromatography (HPCCC) using a system consisting of ethyl acetate/n-butanol/ethanol/water (1:1:0.1:2, v/v/v/v). The separation parameters were first performed on the analytical HPCCC and the optimized conditions were then scaled up to preparative HPCCC. A total of 72.1 mg of prim-O-glucosylcimifugin, 27 mg of 4'-O-β-D-glucosyl-5-O-methylvisamminol, 14.1 mg of cimifugin and 1.1 mg of sec-O-glucosylhamaudol were purified from 960 mg of the n-butanol extract of S. divaricata, each at over 90% purity as determined by high-performance liquid chromatography (HPLC). The structures of four compounds were identified by their retention time, the liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) in the positive ion mode, and confirmed by NMR. The characteristic LC-ESI-MS fragmentation patterns of the four compounds were discussed, and found to be a very specific and useful tool for the structural identification of chromones from S. divaricata.
    Journal of Separation Science 03/2011; 34(5):520-6. · 2.59 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The animal models were conducted to activity screening of different ethanol fraction from Radix Astragali. Eluted from the macroporous adsorptive resins, the potential active fraction that had vasodilative effects was isolated and purified by high-speed counter-current chromatography (HSCCC) using gradient elution. The solvent system was composed of chloroform-methanol-acetic acid-water (2 : 1 : 1 : 1, v/v/v/v). The flow rate was 1.0 mL min(-1) initially and changed to 2.0 mL min(-1) at 300 min. Four flavonoids named calycosin-7-O-β- D -glycoside (a), formononetin-7-O-β- D -glycoside (b), calycosin (c) and formononetin (d) with purity over 94% were obtained and then identified by (1)H NMR and (13)C NMR.
    Natural product research 05/2012; · 1.01 Impact Factor