Uropathogenic virulence factors in isolates of Escherichia coli from clinical cases of canine pyometra and feces of healthy bitches

Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee 3030, Vic, Australia.
Veterinary Microbiology (Impact Factor: 2.51). 06/2003; 94(1):57-69. DOI: 10.1016/S0378-1135(03)00063-4
Source: PubMed


Escherichia coli is commonly isolated in canine pyometra, but little is known of the virulence factors that may be involved in the precipitation of this disease. The aim of this study was to compare the prevalence of uropathogenic virulence factor (UVF) genes in E. coli isolates from canine pyometra and from feces of healthy bitches to evaluate their role in the pathogenesis of pyometra. E. coli from 23 cases of canine pyometra and from the feces of 24 healthy bitches were analyzed, by polymerase chain reaction, for UVF genes associated with canine and human urinary tract infections (UTIs). The prevalences of UVFs in E. coli from canine pyometra were similar to that in canine and human uropathogenic E. coli. The prevalence of pap was greater (P=0.036) for E. coli from pyometra (52%) than for fecal isolates (21%), and the papGIII allele was present in all pap-containing isolates. The prevalences of genes for alpha-haemolysin and cytotoxic necrotising factor 1 were not significantly higher (P=0.075) in E. coli from pyometra than from feces. The proportion of pyometra strains with >or=3 UVFs was higher (P=0.039) than that of fecal strains, suggesting that possession of >or=3 UVF genes enhances the pathogenicity of the strain. Our findings demonstrate that E. coli associated with canine pyometra are similar to uropathogenic strains, and that operons that encode P fimbriae, alpha-haemolysin and cytotoxic necrotising factor 1 probably enhance the virulence and pathogenicity of the strain in the canine genital tract.

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    • "The importance of fimH in the adherence of E. coli to the canine endometrium has been demonstrated using insertional inactivation (Krekeler et al., 2013). In the present study, afa was not detected in E. coli isolates from canine pyometra; a similar low frequency has been observed previously (Chen et al., 2003; Siqueira et al., 2009; Mateus et al., 2013). Six of eight isolates that were positive for sfaD/E in the present study were also positive for usp, hlyA and cnf-1. "
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    ABSTRACT: Escherichia coli is the most common bacterial agent isolated from canine pyometra. The frequencies of 24 virulence genes and pulsed field gel electrophoresis (PFGE) profiles were determined for 23 E. coli isolates from cases of canine pyometra in Brazil. The frequencies of virulence genes were 91.3% fimH, 91.3% irp-2, 82.6% fyuA, 56.5% iroN, 47.8% traT, 39.1% usp, 34.8% sfaD/E, 34.8% tsh, 30.4% papC, 30.4% hlyA, 26.1% papGIII, 26.1% cnf-1, 21.7% papE/F, 21.7% iss, 17.4% iutA, 17.4% ompT, 17.4% cvaC, 17.4% hlyF, 17.4% iucD, 13.0% iucC, 13.0% astA, 4.3% papGII, 0% afaB/C and 0% papGI. The high frequency of yersiniabactin (fyuA and irp2) and salmochelin (iroN) genes suggests that iron uptake systems might be important in the pathogenesis of canine pyometra. PFGE profiles of 19 isolates were heterogeneous, confirming that E. coli isolates from canine pyometra are unlikely to be epidemic clones.
    The Veterinary Journal 11/2014; 202(2). DOI:10.1016/j.tvjl.2014.08.016 · 1.76 Impact Factor
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    • "Common bacteria found in the uteri of healthy bitches reflect the bacterial flora of the vagina and cervix. Escherichia coli is the pathogen most commonly isolated from bitches with pyometra (Chen et al. 2003; Hagman and K€ uhn 2002; Hagman et al. 2006). The uterine response to the presence of bacteria is largely based on innate immunity. "
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    ABSTRACT: Pyometra is a reproductive disorder very common in bitches over 8 years of age in which physiological effects of progesterone on the uterus play a major role. The traditional therapy for pyometra is ovariohysterectomy. The main advantage of ovariohysterectomy over medical management is that it is both curative and preventive for recurrence of pyometra. However, surgery is associated with the risk of anaesthesia and renders the bitch sterile. During the last 10 years, numerous medical treatments have been proposed to treat both open and closed cervix pyometra. The most effective medical treatment with minor side effects seems to be the repeated administration of aglepristone with or without the additional treatment with low doses of prostaglandins.
    Reproduction in Domestic Animals 06/2014; 49 Suppl 2:28-32. DOI:10.1111/rda.12302 · 1.52 Impact Factor
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    • "α-Haemolysin is a pore-forming toxin that lyses not only erythrocytes [30] but also leukocytes, endothelial, and renal epithelial cells [32]. It has been reported in a great number among E. coli isolates from canine pyometra, as reported 52.0% [7], 34.4% [11], and 34.6% [8], while it was reported in a small number among the E. coli isolates from feces 25.0% [7] and 12.7% [8]. In the present study hly gene was found in the opposite distribution, 16.0% in pyometra (pus) isolates, and 47.3% in feces isolates from the same bitch. "
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    ABSTRACT: Pyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on ∼ 50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans.
    International Journal of Microbiology 03/2014; 2014(8):979584. DOI:10.1155/2014/979584
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