Article
Changing concepts in the surgical management of the cervical node metastasis.
Department of Otolaryngology-Head and Neck Surgery, University of Udine, Policlinico Universitario, Piazzale S. Maria della Misericordia, I-33100, Udine, Italy.
Oral Oncology (impact factor:
2.86).
08/2003;
39(5):429-35.
pp.429-35
Source: PubMed
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Citations (0)
- Cited In (4)
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Article: Fascin overexpression promotes neoplastic progression in oral squamous cell carcinoma.
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ABSTRACT: Fascin is a globular actin cross-linking protein, which plays a major role in forming parallel actin bundles in cell protrusions and is found to be associated with tumor cell invasion and metastasis in various type of cancers including oral squamous cell carcinoma (OSCC). Previously, we have demonstrated that fascin regulates actin polymerization and thereby promotes cell motility in K8-depleted OSCC cells. In the present study we have investigated the role of fascin in tumor progression of OSCC. To understand the role of fascin in OSCC development and/or progression, fascin was overexpressed along with vector control in OSCC derived cells AW13516. The phenotype was studied using wound healing, Boyden chamber, cell adhesion, Hanging drop, soft agar and tumorigenicity assays. Further, fascin expression was examined in human OSCC samples (N = 131) using immunohistochemistry and level of its expression was correlated with clinico-pathological parameters of the patients. Fascin overexpression in OSCC derived cells led to significant increase in cell migration, cell invasion and MMP-2 activity. In addition these cells demonstrated increased levels of phosphorylated AKT, ERK1/2 and JNK1/2. Our in vitro results were consistent with correlative studies of fascin expression with the clinico-pathological parameters of the OSCC patients. Fascin expression in OSCC showed statistically significant correlation with increased tumor stage (P = 0.041), increased lymph node metastasis (P = 0.001), less differentiation (P = 0.005), increased recurrence (P = 0.038) and shorter survival (P = 0.004) of the patients. In conclusion, our results indicate that fascin promotes tumor progression and activates AKT and MAPK pathways in OSCC-derived cells. Further, our correlative studies of fascin expression in OSCC with clinico-pathological parameters of the patients indicate that fascin may prove to be useful in prognostication and treatment of OSCC.BMC Cancer 01/2012; 12:32. · 3.01 Impact Factor -
Article: Fascin overexpression promotes neoplastic progression in Oral Squamous Cell Carcinoma.
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ABSTRACT: BACKGROUND: Fascin is a globular actin cross-linking protein, which plays a major role in forming parallel actin bundles in cell protrusions and is found to be associated with tumor cell invasion and metastasis in various type of cancers including oral squamous cell carcinoma (OSCC). Previously, we have demonstrated that fascin regulates actin polymerization and thereby promotes cell motility in K8-depleted OSCC cells. In the present study we have investigated the role of fascin in tumor progression of OSCC. METHODS: To understand the role of fascin in OSCC development and/or progression, fascin was overexpressed along with vector control in OSCC derived cells AW13516. The phenotype was studied using wound healing, Boyden chamber, cell adhesion, Hanging drop, soft agar and tumorigenicity assays. Further, fascin expression was examined in human OSCC samples (N=131) using immunohistochemistry and level of its expression was correlated with clinico-pathological parameters of the patients. RESULTS: Fascin overexpression in OSCC derived cells led to significant increase in cell migration, cell invasion and MMP-2 activity. In addition these cells demonstrated increased levels of phosphorylated AKT, ERK1/2 and JNK1/2. Our in vitro results were consistent with correlative studies of fascin expression with the clinico-pathological parameters of the OSCC patients. Fascin expression in OSCC showed statistically significant correlation with increased tumor stage (P=0.041), increased lymph node metastasis (P=0.001), less differentiation (P=0.005), increased recurrence (P=0.038) and shorter survival (P=0.004) of the patients. CONCLUSIONS: In conclusion, our results indicate that fascin promotes tumor progression and activates AKT and MAPK pathways in OSCC-derived cells. Further, our correlative studies of fascin expression in OSCC with clinico-pathological parameters of the patients indicate that fascin may prove to be useful in prognostication and treatment of OSCC.BMC Cancer 01/2012; 12(1):32. · 3.01 Impact Factor -
Article: Sentinel Lymph Node Biopsy using blue dye technique in Carcinoma of the Buccal Mucosa
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ABSTRACT: 2 Radiology, and 3 Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi India 4 Currently working as Lab Head, SRL Religere, Varanasi This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Aim: This study was carried out to evaluate the feasibility and role of sentinel lymph node biopsy by using blue dye in identifying occult metastasis in neck in N0 neck patients with carcinoma buccal mucosa. Material and Methods: A prospective study was carried out between 2006 to 2008, a total of 28 patients of carcinoma buccal mucosa with clinically negative neck were recruited. Sentinel lymph node biopsy was carried out by peritumoral injection of 1-2 ml of 2.5% isosulphan blue dye. Any blue lymph node visualized during neck dissection was dissected out and sent for histological examination and immunohistochemistry for cytokeratin was performed using PAP peroxidase and anti-peroxidase method. Results: Sentinel nodes were identified In 27 patients. A total of 37 sentinel nodes were harvested with average of 1.3 lymph nodes per neck, the harvest in our cases was lower than that reported in literature. Of the 37 harvested nodes, 14 were positive and 23 were negative for metastases. One of the 14 positive sentinel nodes (3.7%) patient showed micro metastases that were identified by cytokeratin immunostaining though this node was negative on histology. Out of 14 positive necks level Ib was positive in 6, Level IIa in 6 and Level Ia in 2 cases. Six patients had only sentinel nodes positive for metastases while others also had other positive nodes beside sentinel. Conclusion: Results of the present study show that sentinel node biopsy using the blue dye alone is feasible, it further demonstrates an orderly spread in cancers of the buccal mucosa, none of the patients with negative sentinel nodes had node positivity in other nodes, suggesting a definite role of sentinel node biopsy in N0 cancers of the buccal mucosa. World J Surg Med Radiat Oncol 2012; 1:1-8 Introduction Carcinoma of the buccal mucosa is the commonest tobacco associated cancer among males in India; the incidence is particularly high in North India. This is a locally aggressive malignancy with a low propensity to nodal or distant metastasis. Presence of lymph nodeWorld Journal Of surgical Medical Radiation Oncology. 12/2011; 1(1):1-8.
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