Effect of the desert plant Retama raetam on glycaemia in normal and streptozotocin-induced diabetic rats.
ABSTRACT The effect of the aqueous extract of Retama raetam (RR) on blood glucose levels was investigated in fasting normal and streptozotocin-induced diabetic rats after single and repeated oral administration. The aqueous extract of RR at a dose of 20mg/kg significantly reduced the blood glucose in normal rats 6h after a single oral administration (P<0.005) and two weeks after repeated oral administration (P<0.05). This hypoglycaemic effect is more pronounced in streptozotocin (STZ) diabetic rats (P<0.001). The aqueous extract of RR had no effect on basal plasma insulin levels indicating that the underlying mechanism of RR activity is extra-pancreatic. These findings suggest that the aqueous extract of RR possess significant hypoglycaemic effect in both normal and STZ diabetic rats.
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ABSTRACT: Incidence of type II diabetes is rapidly increasing worldwide. In order to identify complementary or alternative approaches to existing medications, we studied anti-diabetic properties of Berberis lycium-a natural health product recommended for diabetes treatment in kashmir. The hypoglycemic activity of the aqueous and methanolic extracts of this plant at two dose levels of 250 and 500mg/kg b.wt in normal as well as in rats with Alloxan monohydrate induced diabetes was studied. The oral administration of aqueous and methanolic extract causes maximum fall of blood glucose level to 58.41% and 50.80% in diabetic rats respectively. The acute oral toxicity studies of the extracts revealed no toxic effects of the extracts. The extracts also lowered the levels of serum cholesterol, serum triglycerides, serum LDL, serum VLDL, serum SGOT, serum SGPT, and Serum ALP in diabetic rats. The histological studies depict that the extracts have a protective effect on the ß-cells of pancreas in diabetic rats in a dose dependent manner.journal of Pharmacy Research. 04/2011; 44(4):2570-2573.
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ABSTRACT: The objective of the study was to investigate the hypoglycaemic properties of Malaysian cocoa (Theobroma cacao) polyphenols extract in-vivo and insulin sensitivity in-vitro. Cocoa extract (CE) (containing 190 -286 mg total polyphenol per gram extract) was prepared from fermented and roasted (140°C, 20 min) beans by extracting with 80% ethanol in the ratio of 1 to 10. For the in-vivo study, the CE was administered in three dosages (1%, 2%, and 3%) to groups of normal and diabetic rats for a period of 4 weeks by force-feeding. Results showed that dosages of 1% and 3% CE significantly reduced (p < 0.05) plasma glucose levels in the diabetic rats. An in-vitro study (BRIN-BD11 cell lines) was used to evaluate the effect of CE on insulin sensitivity. The results demonstrated that CE at a concentration of 0.1 mg/ml significantly increased (p < 0.05) insulin level compared to the control. The results of this study showed that Malaysian cocoa polyphenol extract have the potential of being an insulin-mimetic agent. Further studies are on-going to elucidate the underlying mechanisms of polyphenols present in CE that contribute to the reduction of plasma glucose levels and insulin mimicking activity.International Food Research Journal. 01/2008; 15.
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ABSTRACT: Effects of ethanolic extracts of alligator pear seed on normal and alloxan-induced diabetic rats were investigated in 6 groups of rats (5 rats per group). Test groups were made diabetic with intra-peritoneal injection of alloxan and treated with 450mg and 900mg/kg body weight of alligator pear seed extract. Two non-diabetic groups were also administered with 450mg and 900mg/kg body weight extract. The levels of blood glucose were examined in all 6 experimental groups. In diabetic rats, blood glucose levels were significantly reduced (p < 0.01) by 46.60 - 55.14 % on consumption of the extracts, with greater effect exhibited by the 900mg/kg extract. In non-diabetic rats, blood glucose levels were reduced by 14.62 - 17.33 % on consumption of the seed extract. Histological studies showed a degenerative effect on the pancreatic islet cells of diabetic rats. The results suggested restorative (protective) effect of the extract on pancreatic islet cells. Administration of ethanolic extract of alligator pear seed may contribute significantly to the reduction of blood glucose levels and can be useful in the management of diabetes.