Ann. N.Y. Acad. Sci.
990: 236–238 (2003).©2003 New York Academy of Sciences.
Blood of a French Cow
Isolated from the
B. LA SCOLA,
AND D. RAOULT
Unité des Rickettsies CNRS UMR-A 6020, IFR 48, Faculté de Médecine,
Université de la Méditerranée, 13385 Marseilles Cedex 05, France
Agence Française de Securite Sanitaire des Aliments, Sophia Antipolis, France
Laboratoire Départemental d’Hygiène, Albi, France
; cattle; cow
of animal-specific species. Together with the reclassification of bacteria of the
from a wide range of mammals including rodents,
isolated from the blood of large ruminants in the United States and in France.
has been described and isolated in four wild roe
deer in Germany.
Our objective in this study was to investigate the presence of
blood of cows from the southwest of France. We have collected 10 blood samples
from cows and cultured these onto blood agar with 5% of blood at 37
atmosphere and examined weekly. We isolated from the blood of these cows one
strain after 6 days of culture; the number of bacteria was of 2
CFU/mL. The other blood samples remain negative after 2 months of culture. Gime-
nez staining revealed tiny, straight bacilli. Tests for production of catalase and oxi-
dase were negative. Identification of the isolate was made using PCR amplification
and DNA sequencing of the 16S rRNA gene with primers and methods previously
The 16S rRNA gene of the isolated strain was amplified and
sequenced and after analysis, the isolate was found to be 100% identical to the newly
strain (Genbank accession number AJ 278187). This
species is very close to
and possesses a flagellum.
tree based on the16S rRNA gene is presented in F
species have been identified as human-specific pathogens, such as
, as well as important zoonotic agents.
species have been previously isolated from the blood of various ruminants including
from wild and domestic ruminants (deer, elk, and beef cattle) in North
and in France,
comprises human-specific pathogens and a growing number
species have been isolated
rabbits, cats and dogs,
, have been
C in a 5% CO
Address for correspondence: Didier Raoult, Unité des Rickettsies, Faculté de Médecine, 27,
Boulevard Jean Moulin, 13385 Marseilles Cedex 5, France. Voice: 33.04.91.32.43.75; fax:
IN A FRENCH COW
the blood of four wild roe deer in Germany.
isolated in the blood of a cow in France. Our result was confirmed
using DNA sequencing of two different target genes. In our case, the delay of detec-
tion of bacteria and number of CFU/mL were similar to that described by Dehio and
colleagues for the reference strain of
Our findings indicate that cattles in Europe may be infected by at least three
inter and intraspecies transmission among ruminants may occur and the role of these
bacteria as zoonotic agents remains to be determined.
To date this is the first isolate of
We thank Pat Kelly and Kelly Johnson for editing the manuscript.
unify the genera
from the order
, D.J., S. O’C
, H.H. W
& A.G. S
, with descriptions of
comb. nov., and to remove the family
. Int. J. Syst. Bact.
. 1993. Proposals to
FIGURE 1. Phylogenetic tree based on 16S rRNA gene showing the position of Bar-
tonella schoenbuchensis isolated in the French cow in relation to the known Bartonella sp.
238ANNALS NEW YORK ACADEMY OF SCIENCES
unify the genera
, R., P. R
, M.Y., R.L. R
host specificity of
Trop. Med. Hyg.
, R., M. K
, R., M. A
7. KORDICK, D.L., B. SWAMINATHAN, C.E. GREENE, et al. 1996. Bartonella vinsonii subsp.
berkhofii subsp.nov., isolated from dogs; Bartonella vinsonii subsp. vinsonii; and
emended description of Bartonella vinsonii. Int. J. Syst. Bact. 46: 704−709.
8. CHANG, C.C., B. CHOMEL, R. KASTEN, et al. 2000. Bartonella spp. isolated from wild
and domestic ruminants in North America. Em. Infect. Dis. 6: 306−311.
9. BREITSCHWERDT, E.B., S. SONTAKKE, A. CANNEDY, et al. 2001. Infection with Bar-
tonella weissii and detection of Nanobacterium antigens in a North Carolina beef
herd. J. Clin. Microbiol. 39: 879−882.
10. BERMOND, D., H.J. BOULOUIS, R. HELLER, et al. 2002. Bartonella bovis Bermond et al.
sp. nov. and Bartonella capreoli sp. nov., isolated from European ruminants. Int. J.
Syst. Evol. Microbiol. 52: 383−390.
11. DEHIO, C., C. LANZ, R. POHL, et al. 2001. Bartonella schoenbuchii sp. nov., isolated
from the blood of wild roe deer Int. J. Syst. Evol. Microbiol. 51: 1557−1565.
12. BIRTLES, R.J. & D. RAOULT. 1996. Comparison of partial citrate synthase gene (gltA)
sequences for phylogenetic analysis of Bartonella species Int. J. Syst. Bact. 46: 891−
13. JACOMO, V., P.J. KELLY & D. RAOULT. 2002. Natural history of Bartonella infections
(an Exception to Koch’s Postulate). Clin. Diagn. Lab Immunol. 9: 8−18.
, R.J., T.G. H
, Y. H
species isolated from the blood of wild rats Int. J. Syst. Bacteriol.
, T. T
in rodents from the southeastern United States. Am. J.
, P. M
species isolated from the blood of wild rabbits. Int. J. Syst. Bacteriol.
, N.A. S
comb. nov., and three new species,
sp. nov., and
& D.H. M
, with descriptions of
. 1995. Proposals to
sp. nov. Int. J.
sp. nov., a
1997. Distribution, diversity, and
sp. nov., a new
, V. X
in stray cats. J. Clin. Microbiol.
. 1997. Prevalence of