Bartonella schoenbuchensis isolated from the blood of a French cow.
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ABSTRACT: Bartonella are worldwide distributed facultative-intracellular bacteria that exhibit an immense genomic diversity across mammal and arthropod hosts. The occurrence of cattle-associated Bartonella species was investigated in the cattle-tail louse Haematopinus quadripertusus and in dairy cattle-blood from Israel. Lice were collected from cattle from two dairy farms during summer 2011, and both lice and cow blood samples were collected from additional 7 farms during the successive winter. The lice were identified morphologically and molecularly using 18S rRNA sequence. Thereafter, they were screened for Bartonella-DNA by conventional and real-time PCR assays, using four partial genetic loci (gltA, rpoB, ssrA and ITS). A potentially novel Bartonella variant, closely related to other ruminant-bartonellae, was identified in 11 out of 13 louse pools collected in summer. In the cattle blood, the prevalence of Bartonella spp. infection was 38%, identified as B. bovis and B. henselae (24% and 12%, respectively). A third genotype, closely related to Bartonella melophagi and Bartonella chomelii (based on the ssrA gene) and to B. bovis (based on the ITS sequence) was identified in a single cow. The relatively high prevalence of these Bartonella species in cattle and the occurrence of phylogenetically diverse Bartonella variants in both cattle and their lice suggest the potential role of this animal system in the generation of Bartonella species diversity.Applied and Environmental Microbiology 06/2014; · 3.95 Impact Factor
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ABSTRACT: SUMMARY The deer ked (Lipoptena cervi) is a haematophagous ectoparasite of cervids that harbours haemotrophic Bartonella. A prerequisite for the vector competence of the deer ked is the vertical transmission of the pathogen from the mother to its progeny and transstadial transmission from pupa to winged adult. We screened 1154 pupae and 59 pools of winged adult deer keds from different areas in Finland for Bartonella DNA using PCR. Altogether 13 pupa samples and one winged adult deer ked were positive for the presence of Bartonella DNA. The amplified sequences were closely related to either B. schoenbuchensis or B. bovis. The same lineages were identified in eight blood samples collected from free-ranging moose. This is the first demonstration of Bartonella spp. DNA in a winged adult deer ked and, thus, evidence for potential transstadial transmission of Bartonella spp. in the species.Epidemiology and Infection 06/2014; · 2.49 Impact Factor
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ABSTRACT: Bartonella bovis has been described in beef and dairy cattle worldwide, however the reported prevalence rates are inconsistent, with large variability across studies (0-89%). This study describes the first isolation and characterization of B. bovis among cattle herds in the Middle East. Blood samples from two beef cattle herds (each sampled thrice) and one dairy herd (sampled twice) in Israel were collected during a 16-months period. Overall, 71 of 95 blood samples (75%) grew Bartonella sp., with prevalence of 78% and 59% in beef and dairy cattle, respectively. High level bacteremia (≥100,000 colony forming units/mL) was detected in 25 specimens (26%). Such high-level bacteremia has never been reported in cattle. Two dairy cows and one beef cow remained bacteremic when tested 60 or 120 days apart, respectively, suggesting that cattle may have persistent bacteremia. One third of animals were infested with ticks. Sequence analysis of a gltA fragment of 32 bacterial isolates from 32 animals revealed 100% homology to B. bovis. Species identification was confirmed by sequence analysis of the rpoB gene. Phylogenetic analysis based on the concatenated sequences of gltA and rpoB demonstrated that the isolates described herein form a monophyletic group with B. bovis strains originating from cattle worldwide. Taken together, the high prevalence of bacteremia, including high-level bacteremia, in beef and dairy cattle, the potential to develop prolonged bacteremia, the exposure of cattle to arthropod vectors, and proximity of infected animals to humans, make B. bovis a potential zoonotic agent.Veterinary Microbiology 07/2014; · 2.73 Impact Factor
Ann. N.Y. Acad. Sci.
990: 236–238 (2003).©2003 New York Academy of Sciences.
Blood of a French Cow
Isolated from the
B. LA SCOLA,
AND D. RAOULT
Unité des Rickettsies CNRS UMR-A 6020, IFR 48, Faculté de Médecine,
Université de la Méditerranée, 13385 Marseilles Cedex 05, France
Agence Française de Securite Sanitaire des Aliments, Sophia Antipolis, France
Laboratoire Départemental d’Hygiène, Albi, France
; cattle; cow
of animal-specific species. Together with the reclassification of bacteria of the
from a wide range of mammals including rodents,
isolated from the blood of large ruminants in the United States and in France.
has been described and isolated in four wild roe
deer in Germany.
Our objective in this study was to investigate the presence of
blood of cows from the southwest of France. We have collected 10 blood samples
from cows and cultured these onto blood agar with 5% of blood at 37
atmosphere and examined weekly. We isolated from the blood of these cows one
strain after 6 days of culture; the number of bacteria was of 2
CFU/mL. The other blood samples remain negative after 2 months of culture. Gime-
nez staining revealed tiny, straight bacilli. Tests for production of catalase and oxi-
dase were negative. Identification of the isolate was made using PCR amplification
and DNA sequencing of the 16S rRNA gene with primers and methods previously
The 16S rRNA gene of the isolated strain was amplified and
sequenced and after analysis, the isolate was found to be 100% identical to the newly
strain (Genbank accession number AJ 278187). This
species is very close to
and possesses a flagellum.
tree based on the16S rRNA gene is presented in F
species have been identified as human-specific pathogens, such as
, as well as important zoonotic agents.
species have been previously isolated from the blood of various ruminants including
from wild and domestic ruminants (deer, elk, and beef cattle) in North
and in France,
comprises human-specific pathogens and a growing number
species have been isolated
rabbits, cats and dogs,
, have been
C in a 5% CO
Address for correspondence: Didier Raoult, Unité des Rickettsies, Faculté de Médecine, 27,
Boulevard Jean Moulin, 13385 Marseilles Cedex 5, France. Voice: 33.04.91.32.43.75; fax:
IN A FRENCH COW
the blood of four wild roe deer in Germany.
isolated in the blood of a cow in France. Our result was confirmed
using DNA sequencing of two different target genes. In our case, the delay of detec-
tion of bacteria and number of CFU/mL were similar to that described by Dehio and
colleagues for the reference strain of
Our findings indicate that cattles in Europe may be infected by at least three
inter and intraspecies transmission among ruminants may occur and the role of these
bacteria as zoonotic agents remains to be determined.
To date this is the first isolate of
We thank Pat Kelly and Kelly Johnson for editing the manuscript.
unify the genera
from the order
, D.J., S. O’C
, H.H. W
& A.G. S
, with descriptions of
comb. nov., and to remove the family
. Int. J. Syst. Bact.
. 1993. Proposals to
FIGURE 1. Phylogenetic tree based on 16S rRNA gene showing the position of Bar-
tonella schoenbuchensis isolated in the French cow in relation to the known Bartonella sp.
238ANNALS NEW YORK ACADEMY OF SCIENCES
unify the genera
, R., P. R
, M.Y., R.L. R
host specificity of
Trop. Med. Hyg.
, R., M. K
, R., M. A
7. KORDICK, D.L., B. SWAMINATHAN, C.E. GREENE, et al. 1996. Bartonella vinsonii subsp.
berkhofii subsp.nov., isolated from dogs; Bartonella vinsonii subsp. vinsonii; and
emended description of Bartonella vinsonii. Int. J. Syst. Bact. 46: 704−709.
8. CHANG, C.C., B. CHOMEL, R. KASTEN, et al. 2000. Bartonella spp. isolated from wild
and domestic ruminants in North America. Em. Infect. Dis. 6: 306−311.
9. BREITSCHWERDT, E.B., S. SONTAKKE, A. CANNEDY, et al. 2001. Infection with Bar-
tonella weissii and detection of Nanobacterium antigens in a North Carolina beef
herd. J. Clin. Microbiol. 39: 879−882.
10. BERMOND, D., H.J. BOULOUIS, R. HELLER, et al. 2002. Bartonella bovis Bermond et al.
sp. nov. and Bartonella capreoli sp. nov., isolated from European ruminants. Int. J.
Syst. Evol. Microbiol. 52: 383−390.
11. DEHIO, C., C. LANZ, R. POHL, et al. 2001. Bartonella schoenbuchii sp. nov., isolated
from the blood of wild roe deer Int. J. Syst. Evol. Microbiol. 51: 1557−1565.
12. BIRTLES, R.J. & D. RAOULT. 1996. Comparison of partial citrate synthase gene (gltA)
sequences for phylogenetic analysis of Bartonella species Int. J. Syst. Bact. 46: 891−
13. JACOMO, V., P.J. KELLY & D. RAOULT. 2002. Natural history of Bartonella infections
(an Exception to Koch’s Postulate). Clin. Diagn. Lab Immunol. 9: 8−18.
, R.J., T.G. H
, Y. H
species isolated from the blood of wild rats Int. J. Syst. Bacteriol.
, T. T
in rodents from the southeastern United States. Am. J.
, P. M
species isolated from the blood of wild rabbits. Int. J. Syst. Bacteriol.
, N.A. S
comb. nov., and three new species,
sp. nov., and
& D.H. M
, with descriptions of
. 1995. Proposals to
sp. nov. Int. J.
sp. nov., a
1997. Distribution, diversity, and
sp. nov., a new
, V. X
in stray cats. J. Clin. Microbiol.
. 1997. Prevalence of