Bartonella schoenbuchensis isolated from the blood of a French cow.
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ABSTRACT: Bartonella bovis is commonly detected in cattle. One B. bovis strain was recently isolated from a cow with endocarditis in the USA, suggesting its role as an animal pathogen. In the present study, we investigated bartonella infections in 893 cattle from five countries (Kenya, Thailand, Japan, Georgia, and Guatemala) and 103 water buffaloes from Thailand to compare the prevalence of the infection among different regions and different bovid hosts. We developed a multi-locus sequence typing (MLST) scheme based on nine loci (16S rRNA, gltA, ftsZ, groEL, nuoG, ribC, rpoB, ssrA, and ITS) to compare genetic divergence of B. bovis strains, including 26 representatives from the present study and two previously described reference strains (one from French cows and another from a cow with endocarditis in the USA). Bartonella bacteria were cultured in 6.8% (7/103) of water buffaloes from Thailand; all were B. bovis. The prevalence of bartonella infections in cattle varied tremendously across the investigated regions. In Japan, Kenya, and the Mestia district of Georgia, cattle were free from the infection; in Thailand, Guatemala, and the Dusheti and Marneuli districts of Georgia, cattle were infected with prevalences of 10-90%. The Bartonella isolates from cattle belonged to three species: B. bovis (n=165), B. chomelii (n=9), and B. schoenbuchensis (n=1), with the latter two species found in Georgia only. MLST analysis suggested genetic variations among the 28 analyzed B. bovis strains, which fall into 3 lineages (I, II, and III). Lineages I and II were found in cattle while lineage III was restricted to water buffaloes. The majority of strains (17/28), together with the strain causing endocarditis in a cow in the USA, belonged to lineage I. Further investigations are needed to determine whether B. bovis causes disease in bovids.PLoS ONE 01/2013; 8(11):e80894. · 3.73 Impact Factor
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ABSTRACT: Infections with Bartonella spp. have been recognized as emerging zoonotic diseases in humans. Large knowledge gaps exist, however, relating to reservoirs, vectors and transmission of these bacteria. We describe identification by culture, PCR and housekeeping gene sequencing of Bartonella spp. in fed, wingless deer keds (Lipoptena cervi), deer ked pupae and in blood samples collected from moose, Alces alces, sampled within the deer ked distribution range in Norway. Direct sequencing from moose blood sampled in a deer ked free area also indicated Bartonella infection but at a much lower prevalence. The sequencing data suggested the presence of mixed infections involving two species of Bartonella within the deer ked range, while moose outside the range appeared to be infected with a single species. Bartonella were not detected or cultured from unfed winged deer keds. The results may indicate that long term bacteremia in the moose represents a reservoir of infection and that L. cervi acts as a vector for spread of infection of Bartonella spp.. Further research is needed to evaluate the role of L. cervi in the transmission of Bartonella to animals and humans and the possible pathogenicity of these bacteria for humans and animals.Applied and environmental microbiology 10/2012; · 3.69 Impact Factor
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ABSTRACT: Melophagus ovinus (sheep ked) is one of the most common ectoparasites that contributes to enormous economic losses in the productivity of sheep in many countries. The present study was conducted from January 2012 to July 2013 on M. ovinus collected from sheep at three sites in Ethiopia. Of the sheep studied, 65.7% (88/134) were infested with M. ovinus. The prevalence of M. ovinus was 76% (76/100), 47% (8/17) and 23.5% (4/17) at the Kimbibit, Chacha and Shano sites, respectively. An overall number of 229 M. ovinus specimens (138 females, 86 males and five pupae) and 554 M. ovinus specimens (272 females, 282 males) were collected from young and adult sheep, respectively. Bartonella DNA was detected in 89% (694/783) of M. ovinus using a quantitative Bartonella genus-specific PCR assay targeting the 16S/23S rRNA intergenic spacer region. The sequencing of the PCR products of fragments of the gltA and rpoB genes showed 99.6-100% and 100% homology, respectively, with B. melophagi. Statistically significant variation was not noted in the overall prevalence of Bartonella DNA between female and male M. ovinus. All of the sheep infested with M. ovinus 100% (88/88) harbored at least one M. ovinus specimen that contained Bartonella DNA. This study highlights that B. melophagi in M. ovinus from sheep in highlands in Ethiopia possibly has certain zoonotic importance.Comparative immunology, microbiology and infectious diseases 11/2013; · 2.99 Impact Factor
Ann. N.Y. Acad. Sci.
990: 236–238 (2003).©2003 New York Academy of Sciences.
Blood of a French Cow
Isolated from the
B. LA SCOLA,
AND D. RAOULT
Unité des Rickettsies CNRS UMR-A 6020, IFR 48, Faculté de Médecine,
Université de la Méditerranée, 13385 Marseilles Cedex 05, France
Agence Française de Securite Sanitaire des Aliments, Sophia Antipolis, France
Laboratoire Départemental d’Hygiène, Albi, France
; cattle; cow
of animal-specific species. Together with the reclassification of bacteria of the
from a wide range of mammals including rodents,
isolated from the blood of large ruminants in the United States and in France.
has been described and isolated in four wild roe
deer in Germany.
Our objective in this study was to investigate the presence of
blood of cows from the southwest of France. We have collected 10 blood samples
from cows and cultured these onto blood agar with 5% of blood at 37
atmosphere and examined weekly. We isolated from the blood of these cows one
strain after 6 days of culture; the number of bacteria was of 2
CFU/mL. The other blood samples remain negative after 2 months of culture. Gime-
nez staining revealed tiny, straight bacilli. Tests for production of catalase and oxi-
dase were negative. Identification of the isolate was made using PCR amplification
and DNA sequencing of the 16S rRNA gene with primers and methods previously
The 16S rRNA gene of the isolated strain was amplified and
sequenced and after analysis, the isolate was found to be 100% identical to the newly
strain (Genbank accession number AJ 278187). This
species is very close to
and possesses a flagellum.
tree based on the16S rRNA gene is presented in F
species have been identified as human-specific pathogens, such as
, as well as important zoonotic agents.
species have been previously isolated from the blood of various ruminants including
from wild and domestic ruminants (deer, elk, and beef cattle) in North
and in France,
comprises human-specific pathogens and a growing number
species have been isolated
rabbits, cats and dogs,
, have been
C in a 5% CO
Address for correspondence: Didier Raoult, Unité des Rickettsies, Faculté de Médecine, 27,
Boulevard Jean Moulin, 13385 Marseilles Cedex 5, France. Voice: 33.04.91.32.43.75; fax:
IN A FRENCH COW
the blood of four wild roe deer in Germany.
isolated in the blood of a cow in France. Our result was confirmed
using DNA sequencing of two different target genes. In our case, the delay of detec-
tion of bacteria and number of CFU/mL were similar to that described by Dehio and
colleagues for the reference strain of
Our findings indicate that cattles in Europe may be infected by at least three
inter and intraspecies transmission among ruminants may occur and the role of these
bacteria as zoonotic agents remains to be determined.
To date this is the first isolate of
We thank Pat Kelly and Kelly Johnson for editing the manuscript.
unify the genera
from the order
, D.J., S. O’C
, H.H. W
& A.G. S
, with descriptions of
comb. nov., and to remove the family
. Int. J. Syst. Bact.
. 1993. Proposals to
FIGURE 1. Phylogenetic tree based on 16S rRNA gene showing the position of Bar-
tonella schoenbuchensis isolated in the French cow in relation to the known Bartonella sp.
238ANNALS NEW YORK ACADEMY OF SCIENCES
unify the genera
, R., P. R
, M.Y., R.L. R
host specificity of
Trop. Med. Hyg.
, R., M. K
, R., M. A
7. KORDICK, D.L., B. SWAMINATHAN, C.E. GREENE, et al. 1996. Bartonella vinsonii subsp.
berkhofii subsp.nov., isolated from dogs; Bartonella vinsonii subsp. vinsonii; and
emended description of Bartonella vinsonii. Int. J. Syst. Bact. 46: 704−709.
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tonella weissii and detection of Nanobacterium antigens in a North Carolina beef
herd. J. Clin. Microbiol. 39: 879−882.
10. BERMOND, D., H.J. BOULOUIS, R. HELLER, et al. 2002. Bartonella bovis Bermond et al.
sp. nov. and Bartonella capreoli sp. nov., isolated from European ruminants. Int. J.
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11. DEHIO, C., C. LANZ, R. POHL, et al. 2001. Bartonella schoenbuchii sp. nov., isolated
from the blood of wild roe deer Int. J. Syst. Evol. Microbiol. 51: 1557−1565.
12. BIRTLES, R.J. & D. RAOULT. 1996. Comparison of partial citrate synthase gene (gltA)
sequences for phylogenetic analysis of Bartonella species Int. J. Syst. Bact. 46: 891−
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, R.J., T.G. H
, Y. H
species isolated from the blood of wild rats Int. J. Syst. Bacteriol.
, T. T
in rodents from the southeastern United States. Am. J.
, P. M
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, N.A. S
comb. nov., and three new species,
sp. nov., and
& D.H. M
, with descriptions of
. 1995. Proposals to
sp. nov. Int. J.
sp. nov., a
1997. Distribution, diversity, and
sp. nov., a new
, V. X
in stray cats. J. Clin. Microbiol.
. 1997. Prevalence of