Article

[Construction and analysis of activity of an HIV-1/bovine immunodeficiency virus chimeric clone cDNA].

Department of Oncogenic Virus and HIV, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology 07/2003; 17(2):143-5. pp.143-5
Source: PubMed

ABSTRACT Chimeric human/bovine immunodeficiency virus (HBIV) cDNA was constructed by replacing HIV tat and LTR with bovine immunodeficiency virus (BIV) tat and LTR to study the activity of BIV tat and LTR in the chimerae.
The target fragments of BIV tat, LTR and HIV gag, pol, env were respectively amplified by using PCR and sequentially inserted into pBluescript SK(+) vector. The chimeric clone was transfected into human MT4 cells. The transcript and gene expression of the HBIV chimeric virus were detected by using RT-PCR and a reverse transcriptase assay, respectively.
BIV tat mRNA and HIV gag mRNA were detected. The reverse transcriptase activity of the chimeric virus was analyzed in the fluctuation curve.
In chimeric HBIV cDNA transfected MT?4 cells, BIV tat and HIV gag were transcripted. The reverse transcriptase of the chimeric virus had biological activity. These data suggest that in MT4 cells, BIV LTR had promoter activity and BIV tat had the function of transactivation in the chimeric virus. The study of the chimeric virus with infectivity is in progress.

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Keywords

4 cells
 
BIV LTR
 
BIV tat
 
BIV tat mRNA
 
bovine immunodeficiency virus
 
chimeric clone
 
Chimeric human/bovine immunodeficiency virus
 
chimeric virus
 
fluctuation curve
 
gene expression
 
HBIV chimeric virus
 
HIV gag
 
HIV gag mRNA
 
HIV tat
 
human MT4 cells
 
MT4 cells
 
pBluescript SK(+)
 
reverse transcriptase
 
reverse transcriptase activity
 
reverse transcriptase assay