Chemopreventive effects of orally administered Nigella sativa oil on the induction and development of 1,2-dimethylhydrazine-induced aberrant crypt foci (ACF), putative preneoplastic lesions for colon cancer, were investigated in Fischer 344 rats. Starting at 6 wk of age, 45 male rats (groups 1-3) were subcutaneously injected with DMH once a week for 3 wk. Group 1 (15 rats) served as a carcinogen control group without N. sativa administration. Group 2 or 3 (15 rats each) were given the oil in the postinitiation stage or in the initiation stage, respectively. Animals of group 4 (11 rats) were injected with 0.9% saline and received N. sativa oil from the beginning until the termination. At sacrifice, 14 wk after the start, the total numbers of ACF as well as those with at least four crypts were significantly reduced in group 2 (P < 0.01). However, treatment with N. sativa oil in the initiation stage (group 3) did not exhibit significant inhibitory effects except on foci with only one aberrant crypt. Immunohistochemical analysis of 5-bromo-2'.-deoxyuridine labeling in colonic crypts revealed the N. sativa oil to have significant antiproliferative activity in both initiation and postinitiation stages and especially in the latter. Histological examination revealed no pathological changes in the liver, kidneys, spleen, or other organs of rats treated with N. sativa. In addition, biochemical parameters of blood and urine as well as body weight gain were not affected. These findings demonstrate that the volatile oil of N. sativa has the ability to inhibit colon carcinogenesis of rats in the postinitiation stage, with no evident adverse side effects, and that the inhibition may be associated, in part, with suppression of cell proliferation in the colonic mucosa.
"For instance, thymoquinone was found to have antioxidant effects in animal models   . Black seed and its chief constituents TQ shows therapeutics role in diseases control including cancers such as pancreatic, osteosarcoma, bladder, breast, colon, skin and lung and other diseases        . Earlier investigators showed that oral administration of TQ was "
[Show abstract][Hide abstract] ABSTRACT: The cancer is probably the most dreaded disease in both men and women and also major health problem worldwide. Despite its high prevalence, the exact molecular mechanisms of the development and progression are not fully understood. The current chemotherapy/radiotherapy regime used to treat cancer shows adverse side effect and may alter gene functions. Natural products are generally safe, effective, and less expensive substitutes of anticancer chemotherapeutics. Based on previous studies of their potential therapeutic uses, Nigella sativa and its constituents may be proved as good therapeutic options in the prevention of cancer. Black seeds are used as staple food in the Middle Eastern Countries for thousands of years and also in the treatment of diseases. Earlier studies have shown that N. sativa and its constituent thymoquinone (TQ) have important roles in the prevention and treatment of cancer by modulating cell signaling pathways. In this review, we summarize the role of N. sativa and its constituents TQ in the prevention of cancer through the activation or inactivation of molecular cell signaling pathways.
Evidence-based Complementary and Alternative Medicine 05/2014; 2014(3):724658. DOI:10.1155/2014/724658 · 1.88 Impact Factor
"A big advantage of volatile oils shows a very clear antimutagenic capacity which could well be linked to anticarcinogenic activity. Recent studies have demonstrated that the prooxidant activity of volatile oils or some of their constituents as also that of some polyphenols is very efficient in reducing local tumor volume or tumor cell proliferation by apoptotic and/or necrotic effects (Salim and Fukushima 2003; Yoo et al. 2005; Kachadourian and Day 2006). Our result revealed that the gamma-irradiated red chili alone decreased epithelial and goblet cell hyperplasia and preneoplastic changes in the colon and dysplastic changes, oval cell proliferation, and Kupffer cell hyperplasia in the liver. "
[Show abstract][Hide abstract] ABSTRACT: The comparative effect of raw red chili and red chili treated by gamma irradiation or microwave pasteurization with or without 5-fluorouracil on experimentally induced colon cancer in rats was investigated. Dimethylhydrazine was used to induce colon cancer which is histologically similar to and comparable with human neoplasm. Histopathological examination of dimethylhydrazine-treated rats demonstrated epithelial and goblet cell hyperplasia as well as preneoplastic changes of the rat colon expressed by loss of polarity of the nuclei and abnormal mitosis. The preneoplastic changes were developed to adenocarcinoma in 80% of cases. The liver showed different pathological pictures that included dysplastic changes, preneoplastic foci, and oval cell proliferation. We observed that red chili with or without 5-fluorouracil inhibited colon adenocarcinoma. The gamma red chili either alone or with 5-fluorouracil was the best groups. The gamma irradiated red chili alone decreased epithelial and goblet cell hyperplasia and preneoplastic changes in colon and dysplastic changes, oval cell proliferation, and Kupffer cell hyperplasia in the liver. The gamma irradiated red chili with 5-fluorouracil inhibited the preneoplastic changes in colon. It was concluded that the sanitization of red chili by gamma irradiation either alone or with 5-fluorouracil was more beneficial from the human health point of view than that by microwave.
"Thymoquinone (TQ), a component of BC seed oil, has demonstrated inhibitory effects on Cox-1 expression and prostaglandin E-2 production; necessary components of the Cox-dependent inflammatory response.34 Similar to our data, TQ and BC oil reduced tumorigenesis and xenograft growth in animal models of colon and prostate cancer.32,35 The anti-inflammatory properties of BC oil may account for the decreased CD4+ T cell populations, however, increased NK or CD8+T cells in SalpIL2-treated animals may suggest a shift in the type of inflammatory response in tumor-bearing mice. "
[Show abstract][Hide abstract] ABSTRACT: Fruit seeds high in antioxidants have been shown to have anticancer properties and enhance host protection against microbial infection. Recently we showed that a single oral dose of Salmonella enterica serovar Typhimurium expressing a truncated human interleukin-2 gene (SalpIL2) is avirulent, immunogenic, and reduces hepatic metastases through increased natural killer cell populations in mice. To determine whether antioxidant compounds enhance the antitumor effect seen in SalpIL2-treated animals, we assayed black cumin (BC), black raspberry (BR), and milk thistle (MT) seed oils for the ability to reduce experimental hepatic metastases in mice. In animals without tumor, BC and BR oil diets altered the kinetics of the splenic lymphocyte response to SalpIL2. Consistent with previous reports, BR and BC seed oils demonstrated independent antitumor properties and moderate adjuvant potential with SalpIL2. MT oil, however, inhibited the efficacy of SalpIL2 in our model. Based on these data, we conclude that a diet high in antioxidant oils promoted a more robust immune response to SalpIL2, thus enhancing its antitumor efficacy.
OncoTargets and Therapy 05/2011; 4:59-69. DOI:10.2147/OTT.S17081 · 2.31 Impact Factor
Yi Zhang, Changwei Lin, Guoqing Liao, Sheng Liu, Jie Ding, Fang Tang, Zhenran Wang, Xingsi Liang, Bo Li, Yangchao Wei, Qi Huang, Xuan Li, Bo Tang
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