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Available from: Dominique Rousset, Jun 27, 2015
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    ABSTRACT: Human Enteroviruses (HEVs), members of the ge-nus Enterovirus, family Picornaviridae, are the major cause of variety illnesses, such as poliomyelitis, acute flaccid paralysis, aseptic meningitis and hand-foot-and-mouth disease, especially in young child-ren. Virus isolation and neutralization tests are usually performed to identify the serotype of HEVs, but these tests are labor intensive and time consum-ing. At present, molecular methods allowing the rap-id and specific detection of HEVs are being used. Since the sequence identity of HEVs VP1 region has been shown to be well correlated with the sero-type, this VP1 sequence is often used for serotyping of HEVs. This study was aimed to identify the circu-lating HEVs among healthy children in Antajaya, one of the regions with poor sanitation in Bogor, directly from stool samples. The Reverse-Transcription seminested Polymerase Chain Reac-tion (RT-snPCR) and CODEHOP specific primers which able to amplify almost all of HEVs VP1 region, was used. The phylogenetic tree was constructed by the neighbor-joining method on the basis of VP1 sequences. A hundred and two samples were col-lected and 53 (52.0%) samples were positive for PCR. Thirty of 53 (56.6%) EVs-positive samples were analyzed and identified as Echovirus 21 (30.0%), Coxsackievirus A24 (23.3%), Coxsackievi-rus B4 (10.0%), Coxsackievirus B3 (10.0%), Echovi-rus 25 (16.6%), Echovirus 9 (3.3%), Echovirus 14 (3.3%) and Coxsackievirus A10 (3.3%). Thus, Cox-sackievirus A24 that might be able to recombine with polioviruses causing vaccine associated para-lytic poliomyelitis was dominantly circulating in the population.
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    ABSTRACT: The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing characterization of isolate K/2002 revealed an S3/S2 recombination event at the 3' end of the VP1 coding region. A recombination event resulted in the introduction of six Sabin 2 amino acid residues in a Sabin 3 genomic background. Furthermore, mutations associated with loss of the attenuated phenotype of Sabin 3 strains have been identified in the genome of isolate K/2002. The data presented here emphasize the need for careful planning of vaccination strategies, which involve stopping OPV administration in regions that are certified to be polio-free.
    FEMS Immunology & Medical Microbiology 05/2008; 52(3):343-51. DOI:10.1111/j.1574-695X.2008.00381.x · 2.55 Impact Factor
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    ABSTRACT: We compared echovirus 11 (E11) strains implicated in a severe epidemic in Hungary in 1989 with the prototype E11 strain Gregory and with other E11 strains, most of which were isolated over the same period in Europe (Finland, The Netherlands, Romania, Russia) from sporadic cases or from environmental water. Partial sequencing indicated that the Hungarian strains were closely related to each other and to most European strains. They were particularly closely related to one Romanian strain associated with a sporadic case of hemiparesis and several Finnish strains isolated from environmental water. Sequencing of the complete genomes of one Hungarian strain, the Romanian strain, and one Finnish strain revealed differences of only a few nucleotides in the 5' half of the genome, including the 5' nontranslated region (5'-NTR) and the capsid coding region. However, significant differences were observed in the nucleotide sequences of the 3' half of the genome (nonstructural viral protein region and 3'-NTR), indicating that these strains evolved recently and independently by genetic recombination with other unknown E11 or enterovirus strains.
    Virology 08/2004; 325(1):56-70. DOI:10.1016/j.virol.2004.04.026 · 3.28 Impact Factor