Article

[Synthesis, cloning, expression and antigenicity of the therapeutic multi-epitope gene of hepatitis B virus and of its products].

Institute of tropic diseases, First Military Medical University, Guangzhou 510515, China.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 09/2003; 23(8):802-5.
Source: PubMed

ABSTRACT To study the synthesis, cloning, expression and antigenicity of therapeutic multi-epitope gene of hepatitis B virus.
The therapeutic multi-epitope gene of hepatitis B virus was synthesized and cloned into the vector pWR450-1, then was expressed in E.coli and the products were purified. The immunogenicity of the expressed protein was analyzed by Western-blotting.
Recombinant plasmid PWR/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E. coli, which showed ideal antigenicity by Western-blotting.
The designed multi-epitope therapeutic gene of hepatitis B virus was proved to be correct and the expressed protein may be a good therapeutic vaccine.

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