Nucleotide sequence and phylogenetic classification of candidate human papilloma virus type 92

Department of Medical Microbiology, Malmö University Hospital, Lund University, Malmö, Sweden.
Virology (Impact Factor: 3.32). 09/2003; 312(2):255-60. DOI: 10.1016/S0042-6822(03)00391-X
Source: PubMed


From a basal cell carcinoma (BCC) the complete genome of candidate human papillomavirus (HPV) type 92 was characterized. Phylogenetically, the candidate HPV 92 was relatively distantly related to other cutaneous HPV types within the B1 group. By quantitative real time PCR, 94 viral copies were present per cell in the BCC and another BCC contained 1 viral copy per cell. Lower copy numbers were found in two solar keratoses (1 copy per 33 cells and 1 copy per 60 cells) and two squamous cell carcinomas (1 copy per 436 cells and 1 copy per 1143 cells). The high viral load of HPV 92 in two BCCs differs from the low amount of HPV DNA reported from nonmelanoma skin cancers.

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Available from: Annika Antonsson, Sep 28, 2015
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    • "HPV-96 belongs to Betapapillomavirus species 5 and was originally isolated from a SCC of the skin [18]. The next phylogenetically closest genotype to HPV-150 and HPV-96 is HPV-92, which belongs to Betapapillomavirus species 4, and was originally isolated from a BCC of the skin [16]. Since these three genotypes seem to have evolved through intra-host duplication [43] and two of them show at least some carcinogenic potential, the ancestral genotype from which these genotypes were derived may have itself been an oncogenic HPV genotype. "
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    ABSTRACT: DNA from two novel HPV genotypes, HPV-150 and HPV-151, isolated from hair follicles of immuno-competent individuals, was fully cloned, sequenced and characterized. The complete genomes of HPV-150 and HPV-151 are 7,436-bp and 7,386-bp in length, respectively. Both contain genes for at least six proteins, namely E6, E7, E1, E2, L2, L1, as well as a non-coding upstream regulatory region located between the L1 and E6 genes: spanning 416-bp in HPV-150 (genomic positions 7,371 to 350) and 322-bp in HPV-151 (genomic positions 7,213 to 148). HPV-150 and HPV-151 are phylogenetically placed within the Betapapillomavirus genus and are most closely related to HPV-96 and HPV-22, respectively. As in other members of this genus, the intergenic E2-L2 region is very short and does not encode for an E5 gene. Both genotypes contain typical zinc binding domains in their E6 and E7 proteins, but HPV-151 lacks the regular pRb-binding core sequence within its E7 protein. In order to assess the tissue predilection and clinical significance of the novel genotypes, quantitative type-specific real-time PCR assays were developed. The 95% detection limits of the HPV-150 and HPV-151 assays were 7.3 copies/reaction (range 5.6 to 11.4) and 3.4 copies/reaction (range 2.5 to 6.0), respectively. Testing of a representative collection of HPV-associated mucosal and cutaneous benign and malignant neoplasms and hair follicles (total of 540 samples) revealed that HPV-150 and HPV-151 are relatively rare genotypes with a cutaneous tropism. Both genotypes were found in sporadic cases of common warts and SCC and BCC of the skin as single or multiple infections usually with low viral loads. HPV-150 can establish persistent infection of hair follicles in immuno-competent individuals. A partial L1 sequence of a putative novel HPV genotype, related to HPV-150, was identified in a squamous cell carcinoma of the skin obtained from a 64-year old immuno- compromised male patient.
    PLoS ONE 07/2011; 6(7). DOI:10.1371/journal.pone.0022529 · 3.23 Impact Factor
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    • "Although most cutaneous samples contain only low amounts (b1 copy/cell) of known viruses (Hazard et al., 2006; Vasiljevic et al., 2008; Weissenborn et al., 2005), there are many examples of skin lesions that do contain high amounts of virus per cell, e.g. HPV1 and 2 (Gissmann et al., 1977; Orth et al., 1977) in skin warts, HPV5 and 8 in skin cancers from epidermodysplasia verucciformis patients (Ostrow et al., 1982; Pfister et al., 1981), HPV92 in a BCC (Forslund et al., 2003) and HPV88 (Kullander et al., 2008) in an SCC. Fig. 1. "
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    ABSTRACT: To expand our knowledge of the genomic diversity of human papillomaviruses (HPVs), we searched for new HPVs in squamous cell carcinomas of the skin (SCC) and seemingly HPV-negative, otherwise typically HPV-associated lesions. We describe the characterization of three novel HPV types. HPV109 was isolated from an SCC, HPV112 from a condyloma and HPV114 from a low-grade cervical lesion. Pairwise alignment of the L1 sequences classified HPV114 to genus alpha species 3, whereas HPV112 defined a new species in the genus gamma. HPV109 had uncertain classification because of a low and about equal similarity in the L1 gene (between 60% and 65%) to different genera. Type-specific real-time PCRs of cervical samples, a majority from women with low grade atypical cytology, (n = 2856) and various cutaneous samples (n = 538), found HPV114 in 1.7% (48/2856) of the genital samples, whereas both HPV109 and 112 were rare viruses found at high viral loads only in their index samples.
    Virology 02/2010; 397(2-397):331-336. DOI:10.1016/j.virol.2009.11.027 · 3.32 Impact Factor
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    • "Taking advantage of the rolling-circle amplification method (Rector et al., 2004), the number of isolated PV types has increased over the past few years. Approximately 150 PV genomes, isolated from humans and different animal species, have been cloned and completely sequenced so far (Van Ranst et al., 1992; Terai et al., 2002; Forslund et al., 2003; Rector et al., 2007). However, the number of known non-human host species is still low (approx. "
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    ABSTRACT: A series of papillomavirus (PV) types have been isolated from different rodent species, and most of them belong to the genus Pipapillomavirus. We isolated and sequenced the complete genome of a novel PV type (designated RnPV) from the oral cavity of the Norway rat (Rattus norvegicus), as well as an L1 gene fragment from hair-follicle cells of the European beaver (Castor fiber). As inferred from amino acid sequence data, RnPV clustered within the beta+gamma+pi+Xi-PV supertaxon as a member of the genus Pipapillomavirus. The closest relatives of RnPV were McPV-2 and MmPV, and time estimates indicated that the genus Pipapillomavirus originated in the late Cenozoic era. The close relationship of RnPV to other murid PV types supports the hypothesis of co-divergence between members of the genus Pipapillomavirus and their hosts. However, the derived Neogene origin of the genus Pipapillomavirus is much younger than has been considered for the Rodentia as the primary hosts, indicating that alternative interpretations of the phylogenetic trees should be conceived.
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