Article

Nucleotide sequence and phylogenetic classification of candidate human papilloma virus type 92

Department of Medical Microbiology, Malmö University Hospital, Lund University, Malmö, Sweden.
Virology (Impact Factor: 3.28). 09/2003; 312(2):255-60. DOI: 10.1016/S0042-6822(03)00391-X
Source: PubMed

ABSTRACT From a basal cell carcinoma (BCC) the complete genome of candidate human papillomavirus (HPV) type 92 was characterized. Phylogenetically, the candidate HPV 92 was relatively distantly related to other cutaneous HPV types within the B1 group. By quantitative real time PCR, 94 viral copies were present per cell in the BCC and another BCC contained 1 viral copy per cell. Lower copy numbers were found in two solar keratoses (1 copy per 33 cells and 1 copy per 60 cells) and two squamous cell carcinomas (1 copy per 436 cells and 1 copy per 1143 cells). The high viral load of HPV 92 in two BCCs differs from the low amount of HPV DNA reported from nonmelanoma skin cancers.

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    • "Although most cutaneous samples contain only low amounts (b1 copy/cell) of known viruses (Hazard et al., 2006; Vasiljevic et al., 2008; Weissenborn et al., 2005), there are many examples of skin lesions that do contain high amounts of virus per cell, e.g. HPV1 and 2 (Gissmann et al., 1977; Orth et al., 1977) in skin warts, HPV5 and 8 in skin cancers from epidermodysplasia verucciformis patients (Ostrow et al., 1982; Pfister et al., 1981), HPV92 in a BCC (Forslund et al., 2003) and HPV88 (Kullander et al., 2008) in an SCC. Fig. 1. "
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    ABSTRACT: To expand our knowledge of the genomic diversity of human papillomaviruses (HPVs), we searched for new HPVs in squamous cell carcinomas of the skin (SCC) and seemingly HPV-negative, otherwise typically HPV-associated lesions. We describe the characterization of three novel HPV types. HPV109 was isolated from an SCC, HPV112 from a condyloma and HPV114 from a low-grade cervical lesion. Pairwise alignment of the L1 sequences classified HPV114 to genus alpha species 3, whereas HPV112 defined a new species in the genus gamma. HPV109 had uncertain classification because of a low and about equal similarity in the L1 gene (between 60% and 65%) to different genera. Type-specific real-time PCRs of cervical samples, a majority from women with low grade atypical cytology, (n = 2856) and various cutaneous samples (n = 538), found HPV114 in 1.7% (48/2856) of the genital samples, whereas both HPV109 and 112 were rare viruses found at high viral loads only in their index samples.
    Virology 02/2010; 397(2-397):331-336. DOI:10.1016/j.virol.2009.11.027 · 3.28 Impact Factor
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    • "Taking advantage of the rolling-circle amplification method (Rector et al., 2004), the number of isolated PV types has increased over the past few years. Approximately 150 PV genomes, isolated from humans and different animal species, have been cloned and completely sequenced so far (Van Ranst et al., 1992; Terai et al., 2002; Forslund et al., 2003; Rector et al., 2007). However, the number of known non-human host species is still low (approx. "
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    Journal of General Virology 08/2009; 90(Pt 11):2609-14. DOI:10.1099/vir.0.012583-0 · 3.53 Impact Factor
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    • "Additionally, HPV-92 has been detected in a fraction of BCCs, raising the possibility of a causal association (Forslund et al., 2007). HPV-92 DNA has also been detected in a fraction of SCCs, solar keratoses and keratoacanthomas of immunocompetent as well as immunocompromised patients (Forslund et al., 2003a, b, c). HPV-92 belongs to species 4 b-PVs (de Villiers et al., 2004) and is phylogenetically distantly related to the oncogenic types HPV-5 and -8, with an amino acid identity of 71 % in the L1 protein (Fig. 1). "
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    ABSTRACT: Evidence of a possible association of cutaneous human papillomavirus (HPV) types, especially members of the genus Betapapillomavirus, and the development of non-melanoma skin cancer (NMSC) is accumulating. Vaccination with virus-like particles (VLPs) consisting of self-assembled L1, the major capsid protein, has been introduced to control anogenital HPV infection. This study examined the serological relationship between betapapillomavirus (beta-PV) types 5 and 8 and the new type HPV-92, which has recently been isolated from a basal cell carcinoma containing a high number of viral genomes. Following expression by recombinant baculoviruses, the L1 protein of HPV-92 self-assembled into VLPs that elicited high-titre antibodies after immunization, similar to VLPs from HPV-5 and -8. Haemagglutination inhibition (HAI) assays were used as a surrogate method for the detection of virion-neutralizing antibodies, which correlates with protection from infection. Antisera raised against HPV-5 and -8 VLPs displayed HAI activity not only against the homologous type, but also against heterologous HPV types 5, 8 and 92, whereas HAI activity of antisera against HPV-92 VLP was restricted to the homologous type. The results of neutralization assays using HPV-5 pseudovirions were consistent with those from HAI assays. Cross-neutralizing immune responses by VLP vaccination against heterologous HPV types may provide broader protection against the multiplicity of HPV types detected in NMSC. If a close link to HPV infection can be conclusively established, these results may provide a basis for further evaluation of VLPs of beta-PVs as candidates for a prophylactic skin-type HPV vaccine, aimed at reducing the incidence of NMSC.
    Journal of General Virology 02/2009; 90(Pt 1):136-43. DOI:10.1099/vir.0.006189-0 · 3.53 Impact Factor
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