Article

Polymorphisms in the IL-4 and IL-4R [alpha] genes and allergic asthma.

Immunology Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. of China.
Clinical Chemistry and Laboratory Medicine (impact factor: 2.15). 07/2003; 41(7):888-92. DOI:10.1515/CCLM.2003.134 pp.888-92
Source: PubMed

ABSTRACT Allergic asthma is caused by genetic and environmental factors that interact to determine disease susceptibility and severity. Several lines of evidence suggest that the interleukin (IL-4 gene and the IL-4-receptor alpha (IL-4Ralpha) gene are involved in the development of atopic diseases. We sought to determine whether two polymorphism sites in IL-4 and IL-4Ralpha chain were associated with allergic asthma in a Chinese population. We obtained DNA and clinical data from allergic asthma patients, which were compared with those of a group of healthy control subjects. The subjects were genotyped for the IL-4 C-590T promoter polymorphism and the IL-4R alpha chain Q576R by polymerase chain reaction-restriction fragment length polymorphism. The results showed that the IL-4 C-590T was not associated with allergic asthma in a Chinese population. However, the IL-4R alpha chain 576R/R was significantly increased in allergic asthma patients compared with control subjects (chi2 = 21.16; p < 0.01), and also total plasma immunoglobulin E (IgE) level was increased in allergic asthma patients. These data suggest that the IL-4R alpha chain 576R/R genotypes confer genetic susceptibility to allergic asthma in Chinese.

0 0
 · 
0 Bookmarks
 · 
23 Views
  • Source
    Article: Polymorphic variants of interleukin-13 R130Q, interleukin-4 T589C, interleukin-4RA I50V, and interleukin-4RA Q576R in allergic rhinitis: A pilot study.
    Aravind Yadav, Gopala Krishnan Govindasamy, Rakesh Naidu
    [show abstract] [hide abstract]
    ABSTRACT: The development of allergic rhinitis is considered to be caused by the complex interactions between genetic predisposition and environmental factors. Polymorphisms in the interleukin (IL)-13/4/4RA pathway have previously been shown to be associated with atopic diseases. The purpose of this study was to determine the association between IL-13 R130Q, IL-4 T589C, IL4 receptor alpha (IL-4RA) I50V, or IL-4RA Q576R polymorphisms and risk of allergic rhinitis in a hospital-based Malaysian population. A case-control pilot study was undertaken and genotyping of these polymorphisms was performed using polymerase chain reaction-restriction fragment length polymorphism on 54 allergic rhinitis patients and 45 healthy individuals. Polymorphism of IL-13 R130Q showed significant difference in genotype (p = 0.048) and allele (p = 0.002) frequencies in allergic rhinitis when compared with healthy controls. Individuals who were GA heterozygotes (adjusted odds ratio [OR(adj)] = 3.567; 95% CI, 1.211-10.509), and carriers of A allele genotype (OR(adj) = 3.686; 95% CI, 1.300-10.451) and A allele (OR(adj) = 3.071; 95% CI, 1.514-6.232) had an elevated risk of developing allergic rhinitis. The genotype and allele frequencies of IL-4 T589C, IL-4RA I50V, and IL-4RA Q576R polymorphisms were not significantly different between the allergic rhinitis patients and normal healthy individuals and did not show an associated risk with allergic rhinitis. Our findings indicate that polymorphic variant of IL-13 R130Q appears to be associated with increased risk for development of allergic rhinitis in a hospital-based Malaysian population but not IL-4 T589C, IL-4RA I50V, and IL-4RA Q576 polymorphisms. Additional studies using larger sample size are required to confirm our findings and its exact role in allergic rhinitis.
    Allergy & rhinology (Providence, R.I.). 01/2012; 3(1):e35-40.
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

Allergic asthma
 
allergic asthma patients
 
atopic diseases
 
Chinese population
 
clinical data
 
disease susceptibility
 
environmental factors
 
genetic susceptibility
 
IL-4 C-590T promoter polymorphism
 
IL-4 gene
 
IL-4-receptor alpha
 
IL-4R alpha chain 576R/R
 
IL-4R alpha chain 576R/R genotypes
 
IL-4R alpha chain Q576R
 
IL-4Ralpha
 
IL-4Ralpha chain
 
interleukin
 
polymorphism sites
 
severity
 
total plasma immunoglobulin E