Soyasapogenol A and B distribution in soybean (Glycine max L. Merr.) in relation to seed physiology, genetic variability, and growing location.

Guelph Center for Functional Foods, Laboratory Services, University of Guelph, Guelph, Ontario, N1H 8J7, Canada.
Journal of Agricultural and Food Chemistry (Impact Factor: 2.91). 10/2003; 51(20):5888-94. DOI: 10.1021/jf0343736
Source: PubMed

ABSTRACT An efficient analytical method utilizing high-performance liquid chromatography (HPLC)/evaporative light scattering detector (ELSD) was developed to isolate and quantify the two major soyasaponin aglycones or precursors in soybeans, triterpene soyasapogenol A and B. Soaking of seeds in water up to 15 h did not change the content of soyasapogenols. Seed germination had no influence on soyasapogenol A content but increased the accumulation of soyasapogenol B. Soyasapogenols were mainly concentrated in the axis of the seeds as compared with the cotyledons and seed coat. In the seedling, the root (radicle) contained the highest concentration of soyasapogenol A, while the plumule had the greatest amounts of soyasapogenol B. In 10 advanced food-grade soybean cultivars grown in four locations in Ontario, total soyasapogenol content in soybeans was 2 +/- 0.3 mg/g. Soyasapogenol B content (1.5 +/- 0.27 mg/g) was 2.5-4.5-fold higher than soyasapogenol A content (0.49 +/- 0.1 mg/g). A significant variation in soyasapogenol content was observed among cultivars and growing locations. There was no significant correlation between the content of soyasapogenols and the total isoflavone aglycones.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Soaking of seed had no significant effect on Group A and Group B saponins, but the cooking completely degraded sapogenol B and a loss of 66.3% in total sapogenol of chickpeas was observed during cooking. The desi and kabuli type chickpeas differed remarkably for their sapogenols. The kabuli types contained high sapogenol A and B than desi types. Saponins are responsible for cholesterol lowering and cancer preventive health benefits.
    Current Advances in Agricultural Sciences. 06/2013; 5(1):141-143.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Ten varying genotypes of lentil viz., DPL 15, DPL 58, DPL 62, PL 4, PL 406, PL 639, VL 1, JL 1, K 75 and Ranjan were subjected to soaking and cooking. Soaking and cooking had significant effect on the sapogenols of lentils. Sapogenols are triterpenoid of saponins and produced on hydrolysis of saponins, which are responsible for protection against cancer and tumor, hypocholesterolemic and hepato-protective benefits of health. Sapogenol A was not affected significantly during soaking as well as cooking of lentils. A reduction of 15.3% in sapogenol B was observed during soaking and complete loss of sapogenol B was noticed during pressure cooking of soaked grain. Total sapogenol decreased by 12.6% and 61.9% during soaking and pressure cooking of grain, respectively. There was a wide variability in sapogenol A, B and total in different genotypes in unprocessed, soaked and pressure cooked grain of lentils. The unprocessed grains of lentils had 198.1 to 332.8 and 362.9 to 452.9 mg/100g sapogenol A and sapogenol B, respectively.
    Indian Journal of Agricultural Sciences 08/2013; 83(8):877-880. · 0.18 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Drug development from natural sources is an important and fast developing area. Natural sources (plants) have been used to cure a range of diseases for Thousands of years. Different online medicinal plant databases provide information about classifications, activities, phytochemicals and structure of phytochemicals in different formats. These databases do not cover all aspects of medicinal plants. MAPS (Medicinal plant Activities, Phytochemicals & structural database) has been constructed with uniqueness that it combines all information in one web resource and additionally provides test targets on which particular plant found to be effective with reference to the original paper as well. MAPS database is user friendly information resource, including the data of > 500 medicinal plants. This database includes phytochemical constituents, their structure in mol format, different activities possessed by the medicinal plant with the targets reported in literature.
    Bioinformation 01/2013; 9(19):993-5. · 0.50 Impact Factor


Available from
May 30, 2014