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Observations on a chicken embryo lethal orphan (CELO) virus.

American Journal of Veterinary Research (Impact Factor: 1.35). 08/1957; 18(68):657-60.
Source: PubMed
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    ABSTRACT: Outbreaks of hydropericardium syndrome (HPS), caused by fowl adenovirus serotype 4 (FAdV-4), have occurred in Korea and caused severe economic loss due to mortality and weight loss. From these outbreaks, several adenoviruses were isolated and identified in samples from broilers, layers, breeders, and native Korean fowl. In pathologic examinations, hydropericardium and multifocal hepatic necrosis, with an intranuclear inclusion body in hepatocytes, were observed. Specific adenovirus particles were also observed in the nucleus of hepatocytes, by electron microscopic examination. Polymerase chain reaction (PCR) analysis of the hexon gene identified all of the isolates as FAdV, serotype 4 and genotype C. To reproduce FAdV-4 field cases, 8- and 52-week-old specific pathogen free (SPF) chicks were infected intramuscularly with the field isolate CBU070244. The mortality rate of infected chicks ranged from 10%–40%, and specific pathologic lesions, such as swollen livers and hydropericardium, were observed. Further studies to determine the prevalence of infection, and analysis of the economic impact to the poultry industry, are needed in the near future. Reporte de Caso—Brote del síndrome de hidropericardio y caracterización molecular de aislados de adenovirus aviares de Corea. Brotes del síndrome hidropericardio causados por adenovirus aviar serotipo 4 han ocurrido en Corea causando graves pérdidas económicas debido a la mortalidad y a los bajos pesos corporales. A partir de pollos de engorde, ponedoras y gallinas nativas de Corea involucradas en estos brotes, se aislaron e identificaron varios adenovirus. En los exámenes patológicos se observó hidropericardio y necrosis hepática multifocal con presencia de cuerpos de inclusión intranucleares en los hepatocitos. Mediante el microscopio electrónico se observaron los adenovirus en el núcleo de los hepatocitos. El análisis de la reacción en cadena por la polimerasa del gen hexón identificó todos los virus aislados como pertenecientes al serotipo 4 y al genotipo C de los adenovirus aviares. Para reproducir la enfermedad, se utilizaron aves libres de patógenos de 8 y 52 semanas de edad, infectándolas por la vía intramuscular con el aislamiento de campo identificado como CBU070244. Los porcentajes de mortalidad de las aves infectadas varió del 10% al 40% y se observaron lesiones específicas como inflamación del hígado e hidropericardio. En el futuro cercano, se necesita realizar estudios adicionales para determinar la prevalencia de la infección y el análisis del impacto económico sobre la industria avícola. Abbreviations: bp = base pairs; CBNU = Chungbuk National University; CELiC = chicken embryo liver cells; CELO = chicken embryolethal orphan; CPE = cytopathic effect; FAdV = fowl adenovirus; FAdV-4 = fowl adenovirus serotype 4; HPS = hydropericardium syndrome; PCR = polymerase chain reaction; REA = restriction enzyme analysis; SPF = specific pathogen free
    Avian Diseases 01/2009; · 1.73 Impact Factor
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    ABSTRACT: Thedeoxyribonucleic acid(DNA)ofchick embryolethal orphan(CELO)virus, an oncogenic avian adenovirus, hada biphasic denaturation profile indicating intra- molecular basecomposition heterogeneity. This was confirmed byshearing theDNA andcentrifuging ittoequilibrium inCs2SO4 inthepresenceofHgCl2whentwo bands were formed. No circular molecules formedwhenCELO virus DNA was annealed, although XDNA formed circles underthesame conditions. No circular molecules werefoundbysedimentation orelectron microscopy whentheDNA was digested withexonuclease IIIandthenannealed, but30to40%ofT7DNA molecules becamecircular undersimilar conditions. Thecomplementary strands ofCELO virus DNA bothappeared tobecontinuous, and,whenCELO DNA was denatured andthenannealed under appropriate conditions, alloftherenatured molecules were linear. Itisconcluded thatCELO virus DNA consists ofa uniquerather than permuted collection oflinear molecules thatlackexposed single-strand comple- mentaryends orduplex terminal repetitions. Theseresults arediscussed inrelation tothereplication ofviral DNA andthetransformation ofhostcells. Thedeoxyribonucleic acid(DNA)ofchick embryo lethal orphan (CELO)virus, anoncogenic avianadenovirus, isa linear double-stranded molecule witha molecular weight of30million daltons andan averagebasecomposition of54% guanine pluscytosine (W.G. Laveretal., Virology, inpress). Thispaperdescribes further studies on thematureformofCELO virus DNA tohelpinunderstanding thereplication ofviral DNA andalsothetransformation ofcells by oncogenicviruses. Double-stranded DNA fromallbacteriophages