Observations on a chicken embryo lethal orphan (CELO) virus.

American Journal of Veterinary Research (Impact Factor: 1.35). 08/1957; 18(68):657-60.
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    ABSTRACT: Outbreaks of hydropericardium syndrome (HPS), caused by fowl adenovirus serotype 4 (FAdV-4), have occurred in Korea and caused severe economic loss due to mortality and weight loss. From these outbreaks, several adenoviruses were isolated and identified in samples from broilers, layers, breeders, and native Korean fowl. In pathologic examinations, hydropericardium and multifocal hepatic necrosis, with an intranuclear inclusion body in hepatocytes, were observed. Specific adenovirus particles were also observed in the nucleus of hepatocytes, by electron microscopic examination. Polymerase chain reaction (PCR) analysis of the hexon gene identified all of the isolates as FAdV, serotype 4 and genotype C. To reproduce FAdV-4 field cases, 8- and 52-week-old specific pathogen free (SPF) chicks were infected intramuscularly with the field isolate CBU070244. The mortality rate of infected chicks ranged from 10%–40%, and specific pathologic lesions, such as swollen livers and hydropericardium, were observed. Further studies to determine the prevalence of infection, and analysis of the economic impact to the poultry industry, are needed in the near future. Reporte de Caso—Brote del síndrome de hidropericardio y caracterización molecular de aislados de adenovirus aviares de Corea. Brotes del síndrome hidropericardio causados por adenovirus aviar serotipo 4 han ocurrido en Corea causando graves pérdidas económicas debido a la mortalidad y a los bajos pesos corporales. A partir de pollos de engorde, ponedoras y gallinas nativas de Corea involucradas en estos brotes, se aislaron e identificaron varios adenovirus. En los exámenes patológicos se observó hidropericardio y necrosis hepática multifocal con presencia de cuerpos de inclusión intranucleares en los hepatocitos. Mediante el microscopio electrónico se observaron los adenovirus en el núcleo de los hepatocitos. El análisis de la reacción en cadena por la polimerasa del gen hexón identificó todos los virus aislados como pertenecientes al serotipo 4 y al genotipo C de los adenovirus aviares. Para reproducir la enfermedad, se utilizaron aves libres de patógenos de 8 y 52 semanas de edad, infectándolas por la vía intramuscular con el aislamiento de campo identificado como CBU070244. Los porcentajes de mortalidad de las aves infectadas varió del 10% al 40% y se observaron lesiones específicas como inflamación del hígado e hidropericardio. En el futuro cercano, se necesita realizar estudios adicionales para determinar la prevalencia de la infección y el análisis del impacto económico sobre la industria avícola. Abbreviations: bp = base pairs; CBNU = Chungbuk National University; CELiC = chicken embryo liver cells; CELO = chicken embryolethal orphan; CPE = cytopathic effect; FAdV = fowl adenovirus; FAdV-4 = fowl adenovirus serotype 4; HPS = hydropericardium syndrome; PCR = polymerase chain reaction; REA = restriction enzyme analysis; SPF = specific pathogen free
    Avian Diseases 01/2009; · 1.73 Impact Factor
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    ABSTRACT: Non-human adenovirus vectors have attractive immunological properties for gene therapy but are frequently restricted by inefficient transduction of human target cells. Using chicken embryo lethal orphan (CELO) virus, we employed a nongenetic mechanism of polymer coating and retargeting with basic fibroblast growth factor (bFGF-pc-CELOluc), a strategy that permits efficient tropism modification of human adenovirus. bFGF-pc-CELOluc showed efficient uptake and transgene expression in chick embryo fibroblasts (CEF), and increased levels of binding and internalization in a variety of human cell lines. Transgene expression was also greater than unmodified CELOluc in PC-3 human prostate cells, although the specific activity (RLU per internalized viral genome) was decreased. In CEF, the specific activity of bFGF-pc-CELOluc was considerably higher than in the human prostate cell line PC-3. Retargeted virus was fully resistant to inhibition by human serum with known adenovirus-neutralizing activity in vitro, while in mice CELOluc was cleared less rapidly from the blood than Adluc following i.v. administration in the presence of adenovirus neutralizing serum. Polymer coating and retargeting with bFGF further reduced rates of clearance for both viruses, suggesting protection against both neutralizing and opsonizing factors. The data indicate that CELO virus may be retargeted to infect human cells via alternative, potentially disease-specific, receptors and resist the effects of pre-existing humoral immunity.
    Gene Therapy 03/2006; 13(4):356-68. · 4.32 Impact Factor
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    ABSTRACT: Avian Adeno viruses and Chicken Anemia Viruses cause serious economic losses to the poultry industry of Pakistan each year. Timely and efficient diagnosis of the viruses is needed in order to practice prevention and control strategies. In the first part of this study, we investigated broilers, breeder and Layer stocks for morbidity and mortality rates due to AAV and CAV infections and any co-infections by examining signs and symptoms typical of their infestation or post mortem examination. In the second part of the study, we developed a duplex PCR assay for the detection of AAV and CAV which is capable to simultaneously detect both the viral types prevalent in Pakistan with high sensitivity and 100% specificity.
    Virology Journal 09/2011; 8:440. · 2.09 Impact Factor

V J Yates