Yunlong Zhuang

Publications (13) View all

• Article: Identification of a new HLA allele, HLA-DRB1*03:80, in a Chinese Han ethnic individual.

  Y Zhang, X-M Nie, Y-L Zhuang, Y-H Song, C-F Zhu
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  ABSTRACT: The new allele DRB1*03:80 has a single nucleotide change at position 239(C→G) in exon 2 from the closest matching allele DRB1*03:06.
  Tissue Antigens 04/2013; 81(4):236-7. · 2.59 Impact Factor
• Article: A novel HLA-B*35 allele, B*35:189 with a new motif GGG at codon 35, identified by sequence-based typing in a Chinese potential donor.

  X-M Nie, Y Zhang, Y-L Zhuang, Y-H Song, C-F Zhu
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  ABSTRACT: B*35:189: one nucleotide change resulting in a new motif GGG at codon 35 in HLA-B.
  Tissue Antigens 09/2012; 80(5):467-9. · 2.59 Impact Factor
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  Article: Human leukocyte antigen-C and killer cell immunoglobulin-like receptor gene polymorphisms among patients with syphilis in a Chinese Han population.

  Yun-Long Zhuang, Gui-Jie Ren, Ke-Li Tian, Xin-Ye Li, Yong-Bao Zhu, Jin-Ling Liu, Gui-Ling Si, Peng Li, Yi Zhang, Li Wang, Wen-Jing Zhang, Da-Jian Wang, Chuan-Fu Zhu
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  ABSTRACT: Syphilis is a sexually transmitted infection caused by the Treponema pallidum subspecies pallidum spirochete bacterium. The killer cell immunoglobulin-like receptors (KIR), interacting with human leukocyte antigens (HLA), regulate the activations of natural killer (NK) cells and certain T-cell subsets in response to microbe infection. The objective of this study was to explore whether KIR and HLA-C gene polymorphisms were associated with syphilis in a Chinese Han population. Polymerase chain reaction with sequence-specific primers (PCR-SSP) method was used to genotype KIR and HLA-C genes in 231 syphilis patients and 247 healthy controls. Framework genes KIR2DL4, KIR3DL2, KIR3DL3 and KIR3DP1 were present in all individuals. The frequencies of KIR2DS3 and KIR3DS1 were higher in syphilis patients than in healthy controls (p = 0.030 and p = 0.038, respectively), while the frequency of KIR2DS5 was higher in healthy controls than in syphilis patients (p = 0.015; OR = 0.575). The homozygote for HLA-C1 allele (HLA-C1C1) was more common in controls compared with syphilis patients (p = 0.030; OR = 0.667). The frequency of individuals with HLA-C1C1 and KIR2DL3 genotype was higher in control group relative to syphilis patient group (p = 0.018; OR = 0.647). These data indicated that KIR2DS3 and KIR3DS1 were more prevalent in syphilis patients than in controls, and that KIR2DS5, HLA-C1C1 and HLA-C1C1-KIR2DL3 were more prevalent in controls than in syphilis patients, respectively. These will require further investigation using functional studies.
  Apmis 10/2012; 120(10):828-35. · 1.99 Impact Factor
• Article: Transcriptional profiles of immature ears and tassels in maize at early stage of water stress

  Y. L. Zhuang, G. J. Ren, Y. Zhu, G. H. Hou, X. Qu, Z. X. Li, G. D. Yue, J. R. Zhang
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  ABSTRACT: In the reproductive organs of maize (Zea mays L.), the changes in transcription that occur during meiosis at early stage of water deficit were characterized. We used oligo microarray analysis, which included 57452 transcripts representing more than 30 000 genes, and combined this with reverse Northern blot analysis. After 1 d stress, 1 809 transcripts were differentially expressed (by 2-fold or greater) with 34 % of them being upregulated in the tassels, while in the ears 861 transcripts were differentially expressed with 41 % being upregulated. Of these, 33 transcripts were upregulated in both organs, including those involved in protective functions, reactive oxygen species detoxification, nitrogen metabolism and gibberellin metabolism. In contrast, the transcripts involved in polyamine and sugar metabolism were downregulated.
  Biologia Plantarum 04/2012; 52(4):754-758. · 1.97 Impact Factor
• Article: Cloning and Characterization of a Maize cDNA Encoding Glutamate Decarboxylase

  Yunlong Zhuang, Guijie Ren, Chunmei He, Xinye Li, Qingmei Meng, Chuanfu Zhu, Rongchun Wang, Juren Zhang
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  ABSTRACT: A putative glutamate decarboxylase (GAD) gene, designated ZmGAD1, was cloned from Zea mays with a combination of reverse-transcriptase polymerase chain reaction (RT-PCR) and bioinformatic approaches. The ZmGAD1 cDNA sequence contained a complete open reading frame encoding a putative protein of 496 amino acids, which contained a pyridoxal-5′-phosphate binding domain and a calmodulin (CaM)-binding domain found in nearly all GADs from plants. Sequence analysis showed that it had highest similarity with rice GAD1. Recombinant ZmGAD1 protein was expressed in Escherichia coli, purified and used to measure enzyme activity, which confirmed ZmGAD1 was really a glutamate decarboxylase gene. Southern blotting analysis suggested that ZmGAD1 was present as a single copy gene in the maize genome. RT-PCR analysis revealed that ZmGAD1 was expressed in all examined tissues including the roots, stems, leaves, ears, and tassels. The expression of the ZmGAD1 gene was upregulated and GAD activity was increased in the leaves and roots after treatment with ABA, MeJA, NaCl, PEG, or cold stress. Several stress-related cis-elements were present in the ZmGAD1 promoter cloned from maize genomic DNA. These results suggested that ZmGAD1 might play an important role in responses to abiotic factors and hormone treatments. KeywordsActivity-Expression-Glutamate decarboxylase-Stress- Zea mays
  Plant Molecular Biology Reporter 04/2012; 28(4):620-626. · 2.45 Impact Factor