Publications (46) View all
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Article: Application of vertical microreactor stack with polystylene microbeads to immunoassay
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ABSTRACT: The advantages of vertical microreactor stack with three-dimensional (3D) structure for immunoassay are discussed. The vertical microreactor stack uses vertical fluid flow operation with multifunctional fluid filters. The multifunction of fluid filter is very effective for micromixing and passive valve operation. The mechanism of micromixing is discussed by using computational fluid dynamics (CFD), and the results suggest that the mixing mechanism based on two main effects. One is Coanda effect and the other is Taylor dispersion induced in enormously high aspect ratio capillary and liquid plug. We demonstrated enzyme reaction with unique repeat mixing operation, to evaluate the micromixing performance of fluid filter. As the results, we proved that the fluid filter has very effective mixing performance. The detection limit, which demonstrated by competition enzyme-linked immunosorvent assay (ELISA), is comparable with recommended detection limit, which suggested by Japanese ministry for the environment.Sensors and Actuators A Physical 12/2012; · 1.80 Impact Factor -
Article: Stacked centrifugal microfluidic device with three-dimensional microchannel networks and multifunctional capillary bundle structures for immunoassay
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ABSTRACT: A stacked-type centrifugal microfluidic device with three-dimensional (3D) microchannel networks and multifunctional capillary bundle structures is developed, and an immunoassay for the detection of immunoglobulin G (IgG) is demonstrated. The device consists of multiple layers of disk-like chips with conventional planar microchannels and vertical capillary bundle structures that are constructed on disks of poly(dimethylsiloxane) and poly(methyl methacrylate). The integration of multiple reactors up to 10 reactors on a disk with 8 cm in diameter is realized by reducing the area of reservoir but increasing the thickness. Moreover the thick optical detection reservoir is also realized for absorption spectroscopy in the 3D centrifugal microfluidics. 3D liquid valving on the vertical capillary bundle structures and liquid transportation through these structures are successfully demonstrated. The results show the successful control of the multistep liquid injection without problems such as blockage of capillary bundle by bubbles that expected to be caused due to 3D structure. The results of the immunoassay show clear response to analyte concentration suggesting successful unit operations in developed 3D centrifugal microfluidic device for immunoassay. Finally, the advantages and impact of the 3D design for the development of high-performance centrifugal microfluidic systems are summarized.Sensors and Actuators B Chemical 05/2012; · 3.90 Impact Factor -
Article: Vertical liquid transportation through capillary bundle structure using centrifugal force
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ABSTRACT: The use of three-dimensional (3D) microstructures is becoming essential attempt to develop next generations’ microdevices, to integrate many modules and various functions, and enhance the performance of device. In this paper, we present a new concept for lab on a chip using 3D structure and centrifugal pumping for integrated functional fluid systems such as high-throughput screening, and point of care testing systems which has stacked multiple structures with 3D-interconnection. The use of 3D structure brings many benefits for above high throughput systems, such as possibility to integrate various modules enabling to perform total assay operation, from sample preparation for biochemical reaction and their detection on one platform. For this concept, the most important key technology is control of a vertical valving and transportation of liquid between different 2D micro channel networks with different height levels. We demonstrated such vertical liquid transportation in 3D micro channel networks through the high aspect ratio capillary bundle filter by controlling spinning speed of device and centrifugal force as a pumping force, and confirmed capillary bundle could be employed as vertical microvalve for 3D fluidic systems using centrifugal force as a pumping method.Microsystem Technologies 04/2012; 16(8):1577-1580. · 0.93 Impact Factor -
Article: Immunoassay using poly-tetrafluoroethylene microstructure in organic solvent
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ABSTRACT: This paper reports the first application of poly-tetrafluoroethylene (PTFE) microstructure, which fabricated by synchrotron This paper reports the first application of poly-tetrafluoroethylene (PTFE) microstructure, which fabricated by synchrotron radiation induced photo-evaporation process, to enzyme-linked immunosorbent assay. The advantages of PTFE microstructure for radiation induced photo-evaporation process, to enzyme-linked immunosorbent assay. The advantages of PTFE microstructure for the development of lab-on-a-chip due to the extremely high-aspect ratio microstructure and chemical stability of PTFE is discussed. the development of lab-on-a-chip due to the extremely high-aspect ratio microstructure and chemical stability of PTFE is discussed. The results of immunoassay shows the successful detection of analyte (mouse IgG) with detection range with 0–100ng/ml. This The results of immunoassay shows the successful detection of analyte (mouse IgG) with detection range with 0–100ng/ml. This result suggests the successful immobilization of antibody (anti-mouse IgG goat antibody) onto the X-ray exposed surface of result suggests the successful immobilization of antibody (anti-mouse IgG goat antibody) onto the X-ray exposed surface of PTFE microstructure and successful demonstration of antigen–antibody reaction in the PTFE microstructure. We also demonstrated PTFE microstructure and successful demonstration of antigen–antibody reaction in the PTFE microstructure. We also demonstrated the detection of polychlorinated biphenyl (PCB). As the result of demonstration, we successfully detected PCB with ranging the detection of polychlorinated biphenyl (PCB). As the result of demonstration, we successfully detected PCB with ranging analyte concentration of 0.1–10ng/ml. analyte concentration of 0.1–10ng/ml.Microsystem Technologies 04/2012; 16(8):1465-1470. · 0.93 Impact Factor -
Article: Design and Fabrication of PTFE-Filled Waveguide Components by SR Direct Etching.
IEICE Transactions. 01/2012; 95-C:122-129.
