Yuan Wen

Sichuan University | SCU

Metastasis is the most lethal attribute of human malignancy. High-level expression of survivin is involved in both carcinogenesis and angiogenesis in cancer. Previous studies indicate that a mutation of the threonine residue at position 34 (Thr34Ala) of survivin generates a dominant-negative mutant that induces apoptosis, inhibits angiogenesis, and...

du.tw Abstract: "T echnology stems from humanity", this sentence is used to symbolize our modern life. It means that the interaction between devices and physical object/human in the real world is gaining more and more attention, and it declaims a natural and intuitive methodology to employ. According to previous idea and living wellife has been a g...

Multiple myelomas (MMs) are etiologically heterogeneous and there are limited treatment options; indeed, current monoclonal antibody therapies have had limited success, so more effective antibodies are urgently needed. Polyclonal antibodies are a possible alternative because they target multiple antigens simultaneously. In this study, we produced p...

Murine studies have shown that immunological targeting of fibroblast activation protein (FAP) can elicit protective immunity in the absence of significant pathology. Fibroblast activation protein is a product overexpressed by tumor-associated fibroblasts (TAF) and is the predominant component of the stoma in most types of cancer. Tumor-associated f...

Lymphatic metastasis plays a critical role in ovarian cancer, indicates poor prognoses and correlates to the majority of cancer deaths. Camptothecin derivatives exhibit promising activity for the treatment of solid tumors because of its specific inhibition of eukaryotic DNA topoisomerase I. Yet, its application is hindered due to extreme water inso...

A high concentration of cisplatin (CDDP) induces apoptosis in many tumor cell lines. CDDP has been administered by infusion to avoid severe toxicity. Recently, it has been reported that changes in survivin expression or function may lead to tumor sensitization to chemical and physical agents. The aim of this study was to determine whether a dominan...

Seven in absentia homolog1 (SIAH1) was reported as a tumor suppressor and played an important role in regulating cell apoptosis. However, its effects on the breast carcinogenesis remain unclear. In this study, our aims were to examine the relationship between SIAH1 and Bcl-2-interacting mediator of cell death (Bim) and to explore the effects of SIA...

Sonic Hedgehog (Shh) plays an essential role in vertebrate organogenesis as well as the development of some cancers, including breast cancer. The aim of the present study was to characterize more precisely its role in breast carcinogenesis and elucidate its regulation mechanisms. The expression of Shh was investigated in 97 breast carcinomas and 22...

Patients with localized prostate cancer can usually achieve initial response to conventional treatment. However, most of them will inevitably progress to advanced disease stage. There is a clear need to develop innovative and effective therapeutics for prostate cancer. Mouse survivin T34A (mS-T34A) is a phosphorylation-defective Thr34 → Ala dominan...

Radiosensitivity of tumors is due to a complex interaction of various factors, it has been reported that survivin also acts as a constitutive and inducible radioresistance factor in a panel of tumor cells and approaches designed to inhibit survivin expression or function may lead to tumor sensitisation to chemical and physical agents. Previously, w...

Seven in absentia homolog 1 (SIAH1), a homologue of Drosophila seven in absentia (Sina), has emerged as a tumor suppressor and plays an important role in regulating cell apoptosis. To investigate the role and possible mechanism of SIAH1 in breast cancer cells, we up-regulated the expression of SIAH1 using pcDNA3-myc-SIAH1 and knocked down SIAH1 usi...

雞傳染性喉頭氣管炎的診斷，除靠疫學、臨床症狀、解剖及組織病理學病 變外，尚須配合一種以上的實驗室檢驗才能加以確診。本實驗開發了以聚 合酵素連鎖反應來偵測傳染性喉頭氣管炎病毒核酸的技術，以期能快速診 斷此病。雞傳染性喉頭氣管炎野外病毒株(埔里株)的0.63 kb HindⅢ片斷 經定序後，設定 1對外側引子進行聚合酵素連鎖反應可得一長度為516 bp 之特異性片斷，其敏感度達1 pg之病毒核酸；取部分產物以另 1對內側巢 式引子進行巢式聚合酵素連鎖反應可得另一長度為260 bp之特異性片斷， 其敏感度則高達1 fg (約相當於 6套病毒基因體所含核酸)。以 6株不同 的野外株及疫苗株病毒核酸為模板進行反應，可證明其能廣泛適用於各病 毒株；再以雞胚胎腎臟細胞、馬立克病毒血清型1、2型、火雞皰疹病...