Yoshifumi Kawanabe

Shizuoka General Hospital · Department of Neurosurgery

Intradural extramedullary (IDEM) ependymoma except for tumors originated from the filum terminale or conus medullaris is rare. The present study showed a case of IDEM ependymoma. A 16-year-old boy was referred to our hospital with a complaint of right hypochondriac pain and motor weakness in his right leg. MRI revealed a solitary intradural tumor a...

Objective: Large vessel occlusion (LVO) stroke during pregnancy is rare but a life-threatening issue for the mother and fetus. We report a rare case of a pregnant woman with congenital protein S deficiency who underwent mechanical thrombectomy. Case Presentation: A 35-year-old woman presented with right hemiplegia and aphasia. The National Institut...

Objective: We report a case of anterior condylar confluence dural arteriovenous fistula (ACC dAVF) in whom venous reflux presentation was converted to the anterior medullary vein (AMV) during the observation period. Case Presentation: A 63-year-old woman with ACC dAVF, which only had anterograde drainage routes, exhibited dizziness during the obser...

Background Blister-like aneurysms (BLAs) arise mostly at the supraclinoid internal carotid artery. We report a rare case of ruptured BLA arising at the P1 segment of the posterior cerebral artery (PCA). Case Description A 34-year-old woman presented with disturbance of consciousness. Computed tomography (CT) of the head showed diffuse subarachnoid...

Objective To assess the efficacy of cervical open-door laminoplasty by hydroxyapatite implant insertion between the lamina and the lateral mass without suturing. Methods All patients who underwent cervical open-door laminoplasty with C2/C7 undermining and insertion of hydroxyapatite implants from C3 to C6 were retrospectively evaluated for surgica...

The present case illustrates the incidental detection of a primary malignant melanoma of the thoracic spinal cord before central nervous system dissemination. A 54-year-old female fell down and bruised the thoracolumbar region. The patient visited a local doctor and underwent magnetic resonance imaging (MRI) of the thoracic and lumbar spine to rule...

The present case illustrates the unexpected occurrence of intradural chordomas that were simultaneously discovered in cranial and spinal locations. A 63-year-old female presented with weakness in the left upper extremity. The patient visited a local doctor and underwent brain computerized tomography (CT). CT revealed a brain tumor, and she was refe...

A 54-year-old man presented with a very rare case of radiation-induced intramedullary spinal cord anaplastic astrocytoma, which developed 37 years after radiotherapy for testicular seminoma. The patient presented with weakness and numbness of the left lower extremity that had gradually aggravated for 3 months. Magnetic resonance imaging demonstrate...

Cilostazol is a phosphodiesterase inhibitor that has been shown to inhibit platelet activation. Endothelin is known to be the most potent endogenous growth promoting and vasoactive peptide. In patients and animal models with stroke, the level of circulating endothelin increases and complicates the recovery progress contributed by vascular constrict...

Object: Spinal intradural extramedullary cavernous angiomas are a rare clinical entity. Only 23 surgically treated cases have been reported. The authors report on a unique case with a thoracic intradural extramedullary cavernous angioma in a patient who presented only sudden onset of abdominal pain at first. Cases: The 52-year-old woman presented w...

Object : Intramedullary hemangioblastomas are relatively rare neoplasms, resection of which is difficult because of abundant blood supply. In this paper, cases of intramedullary hemangioblastomas, and our technique of resection are described. Patients and Methods : During the period from 1998 to 2011, a total of 14 intramedullary hemangioblastomas...

Endothelin-1 is the most potent vasoconstrictor agent currently identified, and it was originally isolated and characterized from the culture media of aortic endothelial cells. Two other isoforms, termed endothelin-2 and endothelin-3, were subsequently identified, along with structural homologues isolated from the venom of Actractapis engaddensis k...

Introduction : In this study, cases of cervical compression myelopathy showing enlargement of the spinal cord and hyperintensity within the spinal cord on magnetic resonance imaging (MRI) were studied to clarify clinical characteristics and provide adequate surgical treatment. Patients and Methods : Between 2003 and 2009, 8 patients were surgically...

Study Design : Observational study with retrospective computerized tomography (CT) analysis and clinical study. Objective : The purpose of this study was to examine the microanatomy of the lamina-facet junction and define the optimal position of the lateral gutter in cervical laminoplasty. Materials and Methods : Sixty patients who underwent cervic...

Object: Spinal intradural arachnoid cysts are relatively uncommon and usually asymptomatic but may produce symptoms by compressing the spinal cord or nerve roots suddenly or progressively. We describe four cases of idiopathic intradural thoracic arachnoid cysts with spastic gait disturbance and discuss the magnetic resonance imaging (MRI) study and...

Pyogenic spondylitis is relatively rare. However, mild cases are sometimes overlooked and it may spontaneously disappear in some cases. Conservative therapy with antibiotics is effective in most patients with inflammation alone. However, decompression (with/without fusion) is required in the presence of spinal cord compression-related symptoms. Whe...

Background : At present, in patients with degenerative cervical spondylosis, anterior decompression or laminoplasty is performed according to the extent of the lesion and the extent of spinal cord compression. Cervical laminoplasty is a useful surgical technique for multi-level disease, but problems have been identified with this procedure, such as...

Objective: The authors present a case with persistent primitive hypoglossal artery (PPHA) associated with a ruptured basilar aneurysm. Clinical presentation: A 58-year-old woman with subarachnoid hemorrhage (SAH) was admitted to our hospital. Her neurological condition was Grade III (Hunt and Kosnic). Cerebral angiography revealed a PPHA and a sacc...

When challenged with extracellular fluid shear stress, vascular endothelial cells are known to release nitric oxide, an important vasodilator. Here, we show that the ability of cultured endothelial cells to sense a low range of fluid shear depends on apical membrane organelles, called cilia, and that cilia are compartments required for proper local...

BACKGROUND When challenged with extracellular fluid‐shear stress, vascular endothelial cells are known to release nitric oxide (NO), an important vasodilator. We here show that this mechanosensing signal transduction depends on apical membrane organelles, termed cilia. METHODS AND RESULTS Cells with Pkd1 null/null or Tg737 orpk/orpk mutation encod...

Cerebral aneurysm is a major cause of subarachnoid hemorrhage, but the mechanisms of its development remain unclear. Mechanical stretch has been reported to induce vascular smooth-muscle cell apoptosis via endothelin B receptors (ETBRs). The objectives of this study were to clarify the expression and localization of ETBR in cerebral aneurysms and t...

Object: Endothelin 1 (ET-1) is a major cause of cerebral vasospasm after subarachnoid hemorrhage (SAH), and extracellular Cal++ influx plays an essential role in ET-1-induced vasospasm. The authors recently demonstrated that ET-1 activates two types of Ca"-permeable nonselective cation channels (designated NSCC-1 and NSCC-2) and a store-operated C...

Voltage-operated Ca2+ channel (VOCCs) play a minor role in the contraction of the rat aorta and the increase in the intracellular free Ca2+ concentration ([Ca2+]1) reduced by endothelin-1 (ET-1) and other agonists. Recent studies using patch clamp techniques show that Ca2+ entry through Ca2+-permeable, nonselective cation channels (NSCCs) plays a c...

This article reviews the types and roles of voltage-independent Ca(2+) channels involved in the endothelin-1 (ET-1)-induced functional responses such as vascular contraction, cell proliferation, and intracellular Ca(2+)-dependent signaling pathways and discusses the molecular mechanisms for the activation of voltage-independent Ca(2+) channels by E...

This paper proposes a simplified method to estimate the dynamic properties and seismic peak responses of a single-degree-of-freedom structure with high viscous damping and hysteretic damping. The dynamic properties of this structure vary according to its velocity and displacement. The method is based on equivalent linearization as well as spectrum...

Endothelin-1 (ET 1) activates two types of Ca2+-permeable non-selective cation channels (designated NSCC-1 and NSCC-2) and a store-operated Ca2+ channel (SOCC) in Chinese hamster ovary cells expressing endothelin-A receptors (CHO-ETAR), which couple with G(q), G(S) and G(12). The purpose of this study was to identify the G proteins involved in the...

Endothelin-1 (ET-1) activates two types of Ca2+- permeable non-selective cation channels (designated NSCC-1 and NSCC-2) and a store-operated Ca2+ channel (SOCC) in Chinese hamster ovary cells expressing endothelin-A receptors (CHOETAR), which couple with Gq, Gs and G12. The purpose of this study was to identify the G proteins involved in the activa...

We recently demonstrated that endothelin-1 (ET-1) activates two types of Ca(2+)-permeable nonselective cation channel (designated NSCC-1 and NSCC-2) and a store-operated Ca(2+) channel (SOCC) in rabbit basilar artery (BA) vascular smooth muscle cells (VSMCs). In this study, we investigated the effects of phosphoinositide 3-kinase (PI3K) on ET-1-ind...

Endothelin-1 (ET-1) is one of the major inducers of vasospasm following subarachnoid hemorrhage (SAH). It is generally accepted that extracellular signal-regulated kinase 1 and 2 (ERK1/2) are involved in ET-1-induced vascular contraction. In addition, ET-1 transactivates epidermal growth factor receptor (EGFR) protein tyrosine kinase (PTK), which l...

We demonstrated recently that norepinephrine activates Ca2+ -permeable nonselective cation channels (NSCCs) in Chinese hamster ovary cells stably expressing alpha1A-adrenergic receptors (CHO-alpha1A). Moreover, extracellular Ca2+ through NSCCs plays essential roles in norepinephrine-induced arachidonic acid release. The purpose of the present study...

We recently demonstrated that endothelin-1 (ET-1) activates two types of Ca2+-permeable nonselective cation channels (designated NSCC-1 and NSCC-2) in C6 glioma cells. In the present study, we investigated the effects of NSCCs on the ET-1-induced proline-rich tyrosine kinase 2 (PYK2) phosphorylation in C6 glioma cells. In addition, we examined the...

Endothelin-1 (ET-1) activates two types of Ca2+-permeable nonselective cation channels (designated NSCC-1 and NSCC-2) and a store-operated Ca2+ channel (SOCC) in Chinese hamster ovary cells expressing endothelinA receptors (CHO-ETAR). These channels can be distinguished by their sensitivity to Ca2+ channel blockers 1-(beta-[3-(4-methoxyphenyl) prop...

Endothelin-1 (ET-1) activates two types of Ca2+-permeable nonselective cation channels (designated NSCC-1 and NSCC-2) and a store-operated Ca2+ channel (SOCC) in vascular smooth muscle cells (VSMCs). These channels can be distinguished by their sensitivity to Ca2+-channel blockers, SK&F 96365 and LOE 908. LOE 908 is sensitive to NSCC-1 and NSCC-2,...

We recently demonstrated that endothelin-1 (ET-1) activates two types of Ca(2+)-permeable nonselective cation channels (NSCC-1 and NSCC-2) in C6 glioma cells. It is possible to discriminate between these channels by using the Ca(2+) channel blockers SK&F 96365 (1-[beta-(3-[4-methoxyphenyl]propoxy)-4-methoxyphenethyl]-1H-imidazole hydrochloride) and...

Mutations in polycystin 2 (PC2), a Ca2+-permeable cation channel, cause autosomal dominant polycystic kidney disease. Whether PC2 functions in the endoplasmic reticulum (ER) or in the plasma membrane has been controversial. Here we generated and characterized a polyclonal antibody against PC2, determined the subcellular localization of both endogen...

The Ca++ influx into vascular smooth-muscle cells (VSMCs) plays a fundamental role in the development and chronic effects of vasospasm after subarachnoid hemorrhage (SAH). The Ca++-permeable nonselective cation channels (NSCCs) are activated by several endothelium-derived constricting factors such as endothelin 1 (ET-1) and thromboxane A2. Moreover...

We demonstrated recently that endothelin-1 (ET-1) activates two types of Ca²⁺-permeable nonselective cation channels [designated nonselective cation channel (NSCC)-1 and NSCC-2] and a store-operated Ca²⁺ channel (SOCC) in rabbit internal carotid artery vascular smooth muscle cells (ICA VSMCs). These channels can be distinguished by their sensitivit...

We demonstrated recently that endothelin-1 (ET-1) activates two types of Ca(2+)-permeable nonselective cation channels [designated nonselective cation channel (NSCC)-1 and NSCC-2] and a store-operated Ca(2+) channel (SOCC) in rabbit internal carotid artery vascular smooth muscle cells (ICA VSMCs). These channels can be distinguished by their sensit...

We recently demonstrated that endothelin-1 (ET-1) activates two types of Ca(2+)-permeable nonselective cation channel (designated NSCC-1 and NSCC-2) in Chinese hamster ovarian cells expressing endothelin(B) receptor (CHO-ET(B)R). These channels can be discriminated using the Ca(2+) channel blockers, LOE 908 and SK&F 96365. LOE 908 is a blocker of N...

The purpose of this study was to demonstrate the involvement of Ca(2+) influx through voltage-independent Ca(2+) channels (VICCs) in endothelin-1 (ET-1)-induced transactivation of epidermal growth factor receptor protein tyrosine kinase (EGFR PTK) using the Ca(2+) channel blockers LOE-908 and SK&F-96365 in rabbit internal carotid artery vascular sm...

This study attempted to characterize Ca2+ channels involved in endothelin-1-induced contraction of rabbit basilar artery using whole-cell patch-clamp and measurement of intracellular free Ca2+ concentration. Endothelin-1 activates two types of Ca2+-permeable nonselective cation channels (NSCC-1 and NSCC-2) and a store-operated Ca2+ channel (SOCC) i...

We recently demonstrated that endothelin-1 (ET-1) activates two types of Ca(2+)-permeable nonselective cation channel (designated NSCC-1 and NSCC-2) and a store-operated Ca(2+) channel (SOCC) in Chinese hamster ovary cells expressing endothelin(A) receptor (CHO-ET(A)R). In addition, these channels can be discriminated using Ca(2+) channel blockers...

We recently demonstrated that endothelin-1 (ET-1) activates two types of Ca²⁺-permeable nonselective cation channel (designated NSCC-1 and NSCC-2) and a store-operated Ca²⁺channel (SOCC) in Chinese hamster ovary cells expressing endothelinA receptor (CHO-ETAR). In addition, these channels can be discriminated using Ca²⁺ channel blockers (R,S)-(3,4-...

We recently demonstrated that endothelin-1 (ET-1) activates two types of Ca2+-permeable nonselective cation channels (NSCC-1 and NSCC-2) in Chinese hamster ovary cells expressing endothelinB receptors (CHO-ETBR) that couple with Gq and Gi. The purpose of the present study was to identify the G proteins involved in the activation of these Ca2+ chann...

Endothelin-1 (ET-1) has been proven to activate two types of Ca2+-permeable nonselective cation channels (designated NSCC-1 and NSCC-2) and a store-operated Ca2+ channel (SOCC) in rabbit internal carotid artery vascular smooth muscle cells (ICA VSMCs). Ca2+ influx through these channels plays an essential role for ET-1-induced mitogenesis in ICA VS...

We demonstrated recently that in Chinese hamster ovary cells stably expressing human recombinant endothelin(A) receptors (CHO-ET(A)R), endothelin-1 (ET-1) activates two types of Ca2+-permeable nonselective cation channels (designated NSCC-1 and NSCC-2) and a store-operated Ca2+ channel (SOCC), which can be distinguished by Ca(2+) channel blockers s...

We have recently shown that noradrenaline induces extracellular Ca(2+) influx through nonselective cation channel (NSCC) in Chinese hamster ovary cells expressing alpha(1A)-adrenoceptors (CHO-alpha(1A)). Moreover, this NSCC is sensitive to (R,S)-(3,4-dihydro-6,7-dimethoxy-isoquinoline-1-yl)-2-phenyl-N,N-di-[2-(2,3,4-trimethoxyphenyl)ethyl]-acetamid...

The purposes of the present study were to clarify the significance of the palmitoylation site and the cytoplasmic tail of the endothelin(A) receptor (ET(A)R) in coupling with G proteins and to determine the subtypes of G protein that are involved in actin stress-fiber formation in Chinese hamster ovary cells that stably express ET(A)R (CHO-ET(A)R)....

Endothelin (ET)-1 activates two types of Ca(2+)-permeable nonselective cation channels (NSCC-1 and NSCC-2) and a store-operated Ca(2+) channel (SOCC) in rabbit internal carotid artery (ICA) vascular smooth muscle cells (VSMCs) in addition to the voltage-operated Ca(2+) channel (VOCC). These channels can be discriminated using the Ca(2+) channel blo...

We have recently shown that endothelin-1 activates two types of Ca2+-permeable nonselective cation channels (NSCC-1 and NSCC-2) in C6 glioma cells. These channels can be distinguished by their sensitivity to blockers of the receptor-operated Ca2+ channel, 1-[b-(3-[4-methoxyphenyl]propoxy)-4-methoxyphenethyl]-1H-imidazole hydrochloride (SK&F 96365)...

Cerebral vasospasm after subarachnoid hemorrhage appears when infiltration of macrophages is prominent, however, a causal relationship between the vasospasm and infiltration of macrophages has not been elucidated. A potent vasoconstrictor endothelin is reported to be involved in the cerebral vasospasm, while the origin of the endothelin is unknown....

We constructed Chinese hamster ovary (CHO) cells stably expressing alpha(1A)-, alpha(1B)-, or alpha(1D)-adrenergic receptors (CHO-alpha(1A), CHO-alpha(1B), or CHO-alpha(1D), respectively) and compared the Ca(2+) channels activated by noradrenaline (NA) in these cells using whole-cell recordings and monitoring of the intracellular free Ca(2+) concen...

Ca(2+) channels activated by endothelin-1 (ET-1) in C6 glioma cells (C6 cells) were characterized using whole-cell patch-clamps and by monitoring the intracellular free Ca(2+) concentration ([Ca(2+)](i)), when administering Ca(2+) channel blockers such as LOE 908 and SK&F 96365. Using this methodology, the Ca(2+) channels involved in ET-1-induced m...

We sought to examine the effects of endothelin-1 on the intracellular free Ca2+ concentration ([Ca2+](i)) and mitogenic response in the neuroblastoma cell line, B103 (B103 cells). The results obtained from an [I-125]endothelin-1 binding assay demonstrated that B103 cells express the endothelin receptor. The B-max and K-d values for [I-125]endotheli...

Ca(2+) channels involved in the endothelin-1-induced mitogenic response of cultured rat thoracic aorta smooth muscle cells, A7r5 cells, were characterized using the Ca(2+) channel blockers, LOE 908 and SK&F 96365. Stimulation of A7r5 cells with endothelin-1 induced a mitogenic response as well as a biphasic increase in the intracellular-free Ca(2+)...

This retrospective analysis was undertaken to evaluate a possible relationship between coil packing densitiy and coil compaction on intracranial aneurysms embolized using Guglielmi detachable coils (GDCs). Of the patients who underwent endovascular surgery using GDC in our hospital between 1994 and 1998, 33 patients had endovascular treatment with...

We compared Ca2+ channels activated by endothelin-1 (ET-1) in CHO cells expressing endothelin type A or B receptors (CHO-ETAR or CHO-ETBR) using whole-cell recordings and monitoring of the intracellular free Ca2+ concentration ([Ca2+]i) combined with the use of Ca2+ channel blockers, LOE 908 and SK&F 96365. In both cells, the low concentration (0.1...

We have recently shown that endothelin-1 (ET-1) activates two types of Ca2+-permeable nonselective cation channels (designated NSCC-1 and NSCC-2) and store-operated Ca2+ channel (SOCC). These channels can be pharmacologically discriminated using 1-[beta-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl]-1-H-imidazoe l hydrochloride (SK&F 96365) (a bl...

Results of cervical carotid stenting are reported. Twenty-nine lesions in 25 cases were treated by percutaneous transluminal balloon angioplasty combined with stent placement. All the lesions were successfully dilated. There was one embolic complication during the operation that caused deterioration of preoperative symptoms. Stent deformation causi...

The cardioprotective activity of propranolol is believed to be independent of its beta-adrenoceptor antagonistic effect. Propranolol exerts this effect through a direct effect on the cardiac muscle, but the precise mechanism remains unclear. In this work, we demonstrated that propranolol binds to S100ao and S100L proteins with ED50 of approximately...