Questions and Answers (8) View all
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Answer added in Bacteriophage6 Lytic bacteriophage for Vibrio fischeriDear Robert Armon. I tried to find the email adresses of Prof Jonathan Koon but didn't have much sucess. Could you please reference me a article of hi... [more]
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Question asked in Phage Display TechnologyOpen ProsequencingDoes anyone know how to analyse the data obtained from pyrosequencing of virus?
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Answer added in Bacteriophage18 Which is the best way to concentrate bacteriophages in order to increase phage concentration (PFU/mL)?Dear Michael Szardenings, I work with different phages: that infect vibrios, e.coli, aeromonas. So I am trying to determine an easy and general protoc... [more]
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Question asked in Bacteriophage6 Lytic bacteriophage for Vibrio fischeriI am working on the inactivation of bioluminescent bacteria by bacteriophages. At the moment, the aim of my work is to isolate a lytic phage that infe... [more]
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Answer added in Bacteriophage18 Which is the best way to concentrate bacteriophages in order to increase phage concentration (PFU/mL)?Dear Robert Armon, I have a question...Could I use meat extract instead of beef extract? The beef extract I have is quiet old. Thanks
Publications (2) View all
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Article: Identification and characterization of bacteria with antibacterial activities isolated from seahorses (Hippocampus guttulatus).
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ABSTRACT: The Journal of Antibiotics advance online publication, 26 March 2010; doi:10.1038/ja.2010.27.The Journal of Antibiotics 03/2010; 63(5):271-4. · 1.65 Impact Factor -
Article: Bacteriophages with potential for inactivation of fish pathogenic bacteria: survival, host specificity and effect on bacterial community structure.
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ABSTRACT: Phage therapy may represent a viable alternative to antibiotics to inactivate fish pathogenic bacteria. Its use, however, requires the awareness of novel kinetics phenomena not applied to conventional drug treatments. The main objective of this work was to isolate bacteriophages with potential to inactivate fish pathogenic bacteria, without major effects on the structure of natural bacterial communities of aquaculture waters. The survival was determined in marine water, through quantification by the soft agar overlay technique. The host specificity was evaluated by cross infection. The ecological impact of phage addition on the structure of the bacterial community was evaluated by DGGE of PCR amplified 16S rRNA gene fragments. The survival period varied between 12 and 91 days, with a higher viability for Aeromonas salmonicida phages. The phages of Vibrio parahaemolyticus and of A. salmonicida infected bacteria of different families with a high efficacy of plating. The specific phages of pathogenic bacteria had no detectable impact on the structure of the bacterial community. In conclusion, V. parahaemolyticus and A. salmonicida phages show good survival time in marine water, have only a moderated impact on the overall bacterial community structure and the desired specificity for host pathogenic bacteria, being potential candidates for therapy of fish infectious diseases in marine aquaculture systems.Marine Drugs 01/2011; 9(11):2236-55. · 3.85 Impact Factor
