Wolfram Brune

Heinrich Pette Institute · Dept. of Virology and Immunology

Virology, Biology, Medicine

  • 96Impact points

  • 27Publications

  • 54Followers

Education

Apr, 1993 - Nov, 1993
University of Maryland
Medicine
Baltimore, United States of America
Apr, 1988 - May, 1994
University of Heidelberg
Medicine
Heidelberg, Germany
Languages:
German (native)
English (fluent)
French (basic knowledge)
Russian (basic knowledge)

Contact Details

Business:
Nordufer 20, 20251 Hamburg, Germany
Institution:
Heinrich Pette Institute, Dept. of Virology and Immunology
Telephone:
+49 40 48051 351
Mobile phone:
+49 178 4528945
Website:
http://www.hpi-hamburg.de

Publications

  • Construction of a lytically replicating Kaposi's sarcoma-associated herpesvirus (KSHV)

    Authors: Matthias Budt, Tsvetana Histozova, Georg Hille, Katrin Berger, Wolfram Brune

    Journal of virology. 07/2011;

    KSHV is found predominantly in a latent state in most cell types, impeding investigations of the lytic replication cycle. Here we engineered the cloned KSHV genome, BAC36, to enforce constitutive
  • Inhibition of programmed cell death by cytomegaloviruses

    Authors: Wolfram Brune

    Virus research. 10/2010;

    The elimination of infected cells by programmed cell death (PCD) is one of the most ancestral defense mechanisms against infectious agents. This mechanism should be most effective against
  • Mutations in the M112/M113 coding region facilitate murine cytomegalovirus replication in human cells

    Authors: Uwe Schumacher, Wiebke Handke, Igor Jurak, Wolfram Brune

    Journal of virology. 06/2010;

    Cytomegaloviruses, representatives of the Betaherpesvirinae, cause opportunistic infections in immunocompromised hosts. They infect various cells and tissues in their natural host, but are highly
  • Deletion of the rat cytomegalovirus immediate early 1 gene results in a virus capable of establishing latency but with lower levels of acute virus replication and latency that compromise reactivation efficiency

    Authors: Gordon R. Sandford, Uwe Schumacher, Jakob Ettinger, Wolfram Brune, Gary S Hayward, William H Burns, Sebastian Voigt

    The Journal of general virology. 11/2009;

    The IE1 and IE2 proteins encoded by the major immediate-early (MIE) transcription unit of cytomegaloviruses are thought to play key roles in the switch between latent and lytic cycle infection.
  • Essential role for either TRS1 or IRS1 in human cytomegalovirus replication

    Authors: Emily E Marshall, Craig J Bierle, Wolfram Brune, Adam P Geballe

    Journal of virology. 03/2009;

    Viral infections often produce double-stranded RNA (dsRNA), which in turn triggers potent antiviral responses including the global repression of protein synthesis mediated by protein kinase R (PKR)
  • Tinkering with a viral ribonucleotide reductase

    Authors: David Lembo, Wolfram Brune

    Trends in biochemical sciences. 12/2008;

    Ribonucleotide reductase (RNR), a crucial enzyme for nucleotide anabolism, is encoded by all living organisms and by large DNA viruses such as the herpesviruses. Surprisingly, the beta-herpesvirus
  • Specific inhibition of the PKR-mediated antiviral response by the murine cytomegalovirus proteins m142 and m143

    Authors: Matthias Budt, Lars Niederstadt, Ralitsa S Valchanova, Stipan Jonjic, Wolfram Brune

    Journal of virology. 12/2008;

    Double-stranded (ds) RNA produced during viral infection activates several cellular antiviral responses. Among the best characterized is the shutoff of protein synthesis mediated by the
  • Murine cytomegalovirus m38.5 protein inhibits Bax-mediated cell death

    Authors: Igor Jurak, Uwe Schumacher, Hrvoje Simic, Sebastian Voigt, Wolfram Brune

    Journal of virology. 06/2008; 82(10):4812-22.

    Many viruses encode proteins that inhibit the induction of programmed cell death at the mitochondrial checkpoint. Murine cytomegalovirus (MCMV) encodes the m38.5 protein, which localizes to
  • Inhibition of proinflammatory and innate immune signaling pathways by a cytomegalovirus RIP1-interacting protein

    Authors: Claudia Mack, Albert Sickmann, David Lembo, Wolfram Brune

    Proceedings of the National Academy of Sciences of the United States of America. 03/2008; 105(8):3094-9.

    TNFalpha is an important cytokine in antimicrobial immunity and inflammation. The receptor-interacting protein RIP1 is an essential component of the TNF receptor 1 signaling pathway that mediates the
  • Cloning and sequencing of a highly productive, endotheliotropic virus strain derived from human cytomegalovirus TB40/E

    Authors: Christian Sinzger, Gabriele Hahn, Margarete Digel, Ruth Katona, Kerstin Laib Sampaio, Martin Messerle, Hartmut Hengel, Ulrich Koszinowski, Wolfram Brune, Barbara Adler

    The Journal of general virology. 03/2008; 89(Pt 2):359-68.

    Human cytomegalovirus (HCMV) strain TB40/E, replicates efficiently, exhibits a broad cell tropism and is widely used for infection of endothelial cells and monocyte-derived cells yet has not been
  • Murine cytomegalovirus m142 and m143 are both required to block protein kinase R-mediated shutdown of protein synthesis

    Authors: Ralitsa S Valchanova, Marcus Picard-Maureau, Matthias Budt, Wolfram Brune

    Journal of virology. 11/2006; 80(20):10181-90.

    Cytomegaloviruses carry the US22 family of genes, which have common sequence motifs but diverse functions. Only two of the 12 US22 family genes of murine cytomegalovirus (MCMV) are essential for
  • Induction of apoptosis limits cytomegalovirus cross-species infection

    Authors: Igor Jurak, Wolfram Brune

    The EMBO journal. 07/2006; 25(11):2634-42.

    Cross-species infections are responsible for the majority of emerging and re-emerging viral diseases. However, little is known about the mechanisms that restrict viruses to a certain host species,
  • Manipulating cytomegalovirus genomes by BAC mutagenesis: Strategies and applications

    Authors: Wolfram Brune, Martin Messerle

    Cytomegaloviruses: Molecular Biology and Immunology

    edited by Matthias J. Reddehase, 01/2006: pages 61-69; Caister Academic Press.

    ISBN: 1-904455-02-6

  • SUMOylation of the human cytomegalovirus 72-kilodalton IE1 protein facilitates expression of the 86-kilodalton IE2 protein and promotes viral replication

    Authors: Michael Nevels, Wolfram Brune, Thomas Shenk

    Journal of virology. 08/2004; 78(14):7803-12.

    The 72-kDa immediate-early 1 protein (IE1-72kDa) of human cytomegalovirus has been previously shown to be posttranslationally modified by covalent conjugation to the ubiquitin-related protein SUMO-1.
  • The ribonucleotide reductase R1 homolog of murine cytomegalovirus is not a functional enzyme subunit but is required for pathogenesis

    Authors: David Lembo, Manuela Donalisio, Anders Hofer, Maura Cornaglia, Wolfram Brune, Ulrich Koszinowski, Lars Thelander, Santo Landolfo

    Journal of virology. 05/2004; 78(8):4278-88.

    Ribonucleotide reductase (RNR) is the key enzyme in the biosynthesis of deoxyribonucleotides. Alpha- and gammaherpesviruses express a functional enzyme, since they code for both the R1 and the R2
  • Murine cytomegalovirus m41 open reading frame encodes a Golgi-localized antiapoptotic protein

    Authors: Wolfram Brune, Michael Nevels, Thomas Shenk

    Journal of virology. 12/2003; 77(21):11633-43.

    Viruses have evolved various strategies to prevent premature apoptosis of infected host cells. Some of the viral genes mediating antiapoptotic functions have been identified by their homology to
  • Vaccination of mice with bacteria carrying a cloned herpesvirus genome reconstituted in vivo

    Authors: Luka Cicin-Sain, Wolfram Brune, Ivan Bubic, Stipan Jonjic, Ulrich H. Koszinowski

    Journal of virology. 09/2003; 77(15):8249-55.

    Bacterial delivery systems are gaining increasing interest as potential vaccination vectors to deliver either proteins or nucleic acids for gene expression in the recipient. Bacterial delivery
  • Role of murine cytomegalovirus US22 gene family members in replication in macrophages

    Authors: Carine Ménard, Markus Wagner, Zsolt Ruzsics, Karina Holak, Wolfram Brune, Ann E Campbell, Ulrich H. Koszinowski

    Journal of virology. 06/2003; 77(10):5557-70.

    The large cytomegalovirus (CMV) US22 gene family, found in all betaherpesviruses, comprises 12 members in both human cytomegalovirus (HCMV) and murine cytomegalovirus (MCMV). Conserved sequence
  • Random transposon mutagenesis of large DNA molecules in Escherichia coli

    Authors: Wolfram Brune

    Methods in molecular biology (Clifton, N.J.). 02/2002; 182:165-71.

  • Viral genes involved in immune evasion of cytomegalovirus

    Authors: Ulrich H. Koszinowski, Wolfram Brune

    Nova Acta Leopoldina. 01/1999; 78:199-213.

    Cytomegaloviruses (CMVs) are prototypes of the beta-subgroup of herpesviruses. After immune control of primary CMV infection, viral genomes persist in a latent state for lifetime of the infected
  • Cytomegaloviruses inhibit Bak- and Bax-mediated apoptosis with two separate viral proteins

    Authors: M Çam, Handke Wiebke, M Picard-Maureau, Wolfram Brune

    Apoptosis of infected cells can limit virus replication and serves as an innate defense mechanism against viral infections. Consequently, viruses delay apoptosis by expressing antiapoptotic proteins,
  • Novel GLRA1 Missense Mutation (P250T) in Dominant Hyperekplexia Defines an Intracellular Determinant of Glycine Receptor Channel Gating

    Authors: Brigitta Saul, Thomas Kuner, Diana Sobetzko, Wolfram Brune, Folker Hanefeld, Hans-Michael Meinck, Cord-Michael Becker

    Journal of Neuroscience, v.19, 869-877 (1999).

    Missense mutations as well as a null allele of the human glycine receptor alpha 1 subunit gene GLRA1 result in the neurological disorder hyperekplexia [startle disease, stiff baby syndrome, Mendelian
  • Fast Screening Procedures for Random Transposon Libraries of Cloned Herpesvirus Genomes: Mutational Analysis of Human Cytomegalovirus Envelope Glycoprotein Genes

    Authors: Urs Hobom, Wolfram Brune, Martin Messerle, Gabriele Hahn, Ulrich H. Koszinowski

    We have cloned the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome (BAC) in Escherichia coli. Here, we have subjected the HCMV BAC to random transposon (Tn)
  • Specific Inhibition of the PKR-Mediated Antiviral Response by the Murine Cytomegalovirus Proteins m142 and m143

    Authors: Matthias Budt, Lars Niederstadt, Ralitsa S Valchanova, Stipan Jonjić, Wolfram Brune

    Double-stranded RNA (dsRNA) produced during viral infection activates several cellular antiviral responses. Among the best characterized is the shutoff of protein synthesis mediated by the
  • Mutations in the M112/M113-Coding Region Facilitate Murine Cytomegalovirus Replication in Human Cells

    Authors: Uwe Schumacher, Wiebke Handke, Igor Jurak, Wolfram Brune

    Cytomegaloviruses, representatives of the Betaherpesvirinae, cause opportunistic infections in immunocompromised hosts. They infect various cells and tissues in their natural host but are highly
Follow

Following

Topics

Bi
Me
Academic Degrees

Prof. Dr. med.

Research Keywords

KSHV, Innate Immunity, Cytomegalovirus, Apoptosis, virus - host interaction

Past Advisors

Tom Shenk (postdoc mentor) Ulrich Koszinowski (postdoc mentor) Harald zur Hausen (M.D. thesis advisor)

Current Location

Hamburg, Germany