Skills (39)
Research experience
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Jan 2005–
presentResearch: Paracelsus Medizinische Privatuniversität
Paracelsus Medizinische Privatuniversität · diverse Departments (Dept. Ophthalmology, Dept. Dermatology, &)Austria · SalzburgInterpretation and micrographs (LM&EM) from clinical specimens; included in some publications. -
Jan 1993–
Jan 1995Research: Universität Würzburg
Universität Würzburg · Dept. of Otolaryngology - Head & Neck Surgery · Dr. S. DAZERTGermany · WürzburgTime-limited work-cooperation (no fundings) in an animal expertimental study [cats] on use of 'Ionomer-cement'.
Education
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Oct 1971–
Dec 1980Univ. Salzburg (Alma Mater Paridiana Salisburgensis)
Zoology&Botany · Ph. D. (Doctor of Philosophy)Austria · SALZBURG
Awards & achievements
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Jun 1993Award: 3rd place of the „AK-Umweltschutzpreis“
Other
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LanguagesGerman, English
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Scientific MembershipsOeGEM(AuSEM/ASEM), DGEM, MSA, FRMS, JSM (JapSEM), SCUR, SUP, DGGG, NSH(USA) & others
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Other InterestsSince ~1990 Member (and protocol clerk) of Works Council SALK-LKH (Gen.Hospital Salzburg) with ~ 4000 employees; 'Safety Person';
For now ~100 ToC's: many and most of the leading journals in Dermatology, Neuromuscular Disorders, Eye/Opthalmology and Brain Res; EM (all range) -specific or related journals.
Literature data bank with reference to EM in general, methods and techniques (esp. spec. preparation, resin embedding LM & TEM, staining procedures -LM&TEM, etc.)
[Elected Board-Member of SCUR (Apr 2003-June 2008),
proposal and election by the SCUR General Assembly, 25th April 2003]
LYON (France) 2012-2016: SECRETARY of SCUR (Society for Cutaneous Ultrastructure Research, , ) elected by vote of General Assembly 24th May 2012 visit: www.scur.org.
Dermato-Pathologists and (Ultra-)Structure Researchers: SCUR-SSSR Conference MAY12-14th 2013 SALZBURG see www.scur.org (sublink: <Next Meeting>) Thank you for your visting the website
Questions and Answers (164) View all
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Answer added in Periodontics and Oral Pathology11 What are the proper classifications for periodontal desease?Dear Kerstin, dear posters, I know the thread of this question is relatively old now, but as I got today an issue of the Magazine of the German Resear... [more]
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Answer added in Transmission Electron Microscopy (TEM)6 Questions on visualizing TMV virions using TEMHi Tommy, after the very excellent advice from Tomas I think it needs no more comment except your point <3\ When visualizing, often times, the sampl... [more]
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Answer added in Sectioning4 I need some help with the embedding process of pollen, my lab doesn't have propylene oxide and instead I am using nitrile acetate.Dear Ana Gabriela, I guess you mean "Acetonitrile" (Acetonitrilo, acetonitrilio, AN) as a substitute for Propyleneoxide (also: 1,3-Epoxypropane, "1,... [more]
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Answer added in Staining2 Staining aorta samples with Verhoff-Van Geison's stain; is this stain soluble in PBS?Dear Hadi Taghizadeh, not knowing which staining recipe you will follow - and knowing that almost ALL recipes are written for staining only "sections"... [more]
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Answer added in Brain Imaging15 Has anyone tried the new CLARITY technique out of the Deisseroth lab in Stanford?Dear William, thank you for the new ( correct ) link to CLARITY....(:-)) best wishes and regards, Wolfgang
Publications (69) View all
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Article: Functional correction of type VII collagen expression in dystrophic epidermolysis bullosa.
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ABSTRACT: Functional defects in type VII collagen, caused by premature termination codons on both alleles of the COL7A1 gene, are responsible for the severe autosomal recessive types of the skin blistering disease, recessive dystrophic epidermolysis bullosa (RDEB). The full-length COL7A1 complementary DNA (cDNA) is about 9 kb, a size that is hardly accommodated by therapeutically used retroviral vectors. Although there have been successful attempts to produce functional type VII collagen protein in model systems of RDEB, the risk of genetic rearrangements of the large repetitive cDNA sequence may hamper the clinical application of full-length COL7A1 cDNA in the human system. Therefore, we used trans-splicing to reduce the size of the COL7A1 transcript. Retroviral transduction of RDEB keratinocytes with a 3' pre-trans-splicing molecule resulted in correction of full-length type VII collagen expression. Unlike parental RDEB keratinocytes, transduced cells displayed normal morphology and reduced invasive capacity. Moreover, transduced cells showed normal localization of type VII collagen at the basement membrane zone in skin equivalents, where it assembled into anchoring fibril-like structures. Thus, using trans-splicing we achieved correction of an RDEB phenotype in vitro, which marks an important step toward its application in gene therapy in vivo.Journal of Investigative Dermatology 01/2011; 131(1):74-83. · 6.31 Impact Factor -
Article: Elemental Analysis in Electron Microscopy for Medical Diagnostics
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ABSTRACT: Full Text: The study of specimens in the electron microscopy gives not only information about the structure but also indications about the elemental composition of samples. When an electron beam irradiates a specimen, a number of different interactions occur. Besides others X-rays and inelastically scattered electrons carry information about the elemental composition of the specimen in the region that is being irradiated. Analytical electron microscopes are in most cases combined with an Energy Dispersive X-ray (EDX) system [1]. In transmission electron microscopy there is an additional possibility to use the Electron Energy-Loss Spectroscopy (EELS) by filtering and analyse the inelastically scattered electrons [2]. In the Electron Microscopic Centre (EMZ) of the University of Rostock inside the Department of Pathology, there are a SEM DSM 960 A (Zeiss) with an EDX system (KEVEX) and two TEMs, an EM 902 A (Zeiss) with an EELS system and a Libra 120 with an EELS as well as an EDX system (EDAX). These analytical microscopes are used for scientific and diagnostic purpose. Here, we give some examples for elemental analysis in diagnostic electron microscopy. In ultrapathology the elemental analysis is useful in cases, where we can see deposits of heavy metals or crystals of unknown origin. To verify Wilson disease in patients we analysed liver biopsies and detected copper (Cu) by EDX and EELS/ESI in electron dense lysosomes. There were two cases of manifested Wilson disease with positive detection of mutation and strong macronodular cirrhosis in the end stage and one child with a very early stage of disease [3]. Fig. 1 shows a hepatocyte of a six year old boy without clinical symptomes and not yet detected mutation, but with positive Cu detection inside typical electron dense accumulations in lysosomes. In a further example, we were able to verify a very early stage of haemochromatosis in a liver biopsy of a 12-year old girl with dark lysosomes inside the hepatocytes around bile capillaries with positive detection of iron by EELS and ESI (Fig. 2) [4]. In a 32-year-old woman we detected Cu and S in brown to black deposits inside Actinomyces druses and endometritis after more than 5 years use of Cu-intrauterine pessar. The dark precipitates inside the bacterial colonies and inside the bacterial cells could be identified as copper sulphide and replaced therefore superseded a suspicious diagnosis of endometrium or uterus carcinoma (pictures not shown) [5]. In the last example (case Dr. Muss, Salzburg, see Poster; see also the same Journal, pp.180-181, [@ http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1329908&fulltextType=&fileId= ] and @[https://www.researchgate.net/publication/215629451_Modern_Analytical_Electron_Microscopical_Methods_for_Confirmation_of_Previously_Suspected_-_Morphologically_Established_-_Ultrastructural_Diagnosis_of_an_Inflammatory_Myopathy_in_Human_Skeletal_Muscle_Tissue_Macrophagic_Myofasciitis ] , we detected aluminium (Al) inside muscle macrophages with crystalline precipitations inside the macrophagic lysosomes (Fig. 3). A 28-years old lady was immunized for several times with aluminium containing adjuvants, leading to a myofasciitis with strong pain. References [1] A. Warley: X-ray microanalysis for biologists. In :Practical methods in electron microscopy (ed by A.M. Glauert), Portland Press London 2002 [2] R.F. Egerton: Electron energy-loss spectroscopy in the electron microscope. Plenum Press New York, London 1996 [3] L. Jonas et al. Ultrastruct Pathol 2001; 25: 111-118. [4] L. Jonas et al. Ultrastruct. Pathol 2002 ; 26 : 23-26. [5] L. Jonas et al. Ultrastruct Pathol 2002 ; 26 : 323 329.Microscopy and Microanalysis 09/2007; 13(Suppl.3-http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1330076&fulltextType=&fileId=):222-223. · 3.01 Impact Factor -
Article: Das Excimer Laser Corneal Shaping-(ELCS-)System: hochpräzise Herstellung von Hornhaut- (Cornea-)Transplantaten mittels Excimer Laser-Ablation
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ABSTRACT: Zur Herstellung von hochpräzisen Transplantaten aus menschlicher Hornhaut eignet sich die Abtragung mittels Excimer Laser bei 193 nm Wellenlänge besonders gut. Darauf basiert das Excimer Laser Corneal-Shaping-System (ELCS-S), mit dem aus Spenderhornhäuten in vitro durch Laserablation eine breite Palette verschiedener Dickenprofile herstellbar ist. Durch Verwendung eines unlängst implementierten Abtragungsalgorithmus (OSLA, Optimized Scanning Laser Ablation) konnte die Qualität der bearbeiteten Oberfläche wesentlich verbessert werden, wie anhand von elektronenmikroskopischen Aufnahmen demonstriert wird. Dadurch rückt die Verwendbarkeit von refraktiven Transplantaten (Lentikeln), insbesonders zur Korrektur von Weitsichtigkeit, in greifbare Nähe. To produce highly accurate transplants of human donor corneal tissue, Excimer Laser Ablation using 193 nm wavelength (as employed by the ELCS-System) is a well suited method. By using a recently developed ablation algorithm (Optimized Scanning Laser Ablation, OSLA), the surface smoothness has improved considerably as shown by scanning EM. Excimer laser treated surfaces can now be produced with deviations in smoothness of less than 10 μm.e & i Elektrotechnik und Informationstechnik 04/2012; 117(4):265-268. -
Article: Cerebral localized marginal zone lymphoma presenting as hypothalamic-pituitary region disorder.
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ABSTRACT: Marginal zone B-cell lymphoma is a rare disease which can be considerably difficult to recognize and diagnose when signs of systemic involvement are absent. We report the case of a 57-year-old woman with initial olfactory disturbance, followed by psychosis, diabetes insipidus and hypothalamic eating disorder as an uncommon clinical presentation of marginal zone B-cell lymphoma. Marginal zone B-cell lymphoma should be considered as a potential differential diagnosis in patients with hypothalamic disturbances.Case Reports in Neurology 05/2011; 3(2):129-35. -
Conference Proceeding: Abstracts
SCUR (Society for Cutaneous Ultrastructure Research) Annual Meeting May 30-31,2011, Brisbane, Australia; 01/2011
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