Vinícius Pedrazzi

Publications (34) View all

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  Available from: Vinícius Pedrazzi

  Article: In vivo evaluation of a metronidazole-containing gel for the adjuvant treatment of chronic periodontitis: preliminary results.

  P K Miani, C do Nascimento, S Sato, A V Filho, M J V da Fonseca, V Pedrazzi
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  ABSTRACT: The present study developed an experimental metronidazole-based gel and evaluated its efficacy for the adjuvant treatment of chronic periodontitis. Sixteen patients were randomly allocated into two groups of eight subjects according to the following proposed treatments: (1) scaling and root planing (active control) or (2) scaling and root planing and direct periodontal intrapocket application of 15% metronidazole-based gel in two sites (≥5 mm in depth) (experimental group). Potential changes in the subgingival microbiota were assessed using a DNA Checkerboard method at three proposed times: baseline and following 7 or 30 days of drug administration. High-performance liquid chromatography (HPLC) monitored metronidazole concentrations in the crevicular fluid during treatment. The metronidazole experimental group presented lower bacterial counts than the control group at the three evaluated times (p<0.01 for baseline, p<0.001 for 7 or 30 days) when the target species were analyzed as a pool of bacteria. Samples revealed significantly lower counts 7 days after drug administration compared with baseline or after 30 days (p<0.05). HPLC analysis detected gel 1 h after application. The metronidazole-based gel significantly decreased the total bacterial count at the three evaluated times. Periodontopathogenic species were not different after gel administration.
  European Journal of Clinical Microbiology 12/2011; 31(7):1611-8. · 2.86 Impact Factor
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  Available from: Ricardo Faria Ribeiro

  Article: Leakage of saliva through the implant-abutment interface: in vitro evaluation of three different implant connections under unloaded and loaded conditions.

  Cássio do Nascimento, Paola Kirsten Miani, Vinícius Pedrazzi, Reginaldo Bruno Gonçalves, Ricardo Faria Ribeiro, Adriana Cláudia Lapria Faria, Ana Paula Macedo, Rubens Ferreira de Albuquerque
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  ABSTRACT: Bacterial leakage along the implant-abutment interface, with consequent species harboring the inner parts of two-part dental implant systems, has been reported in the literature. The aim of this in vitro study was to evaluate bacterial leakage from human saliva to the internal part of the implants along the implant-abutment interface under loaded and unloaded conditions using DNA Checkerboard. Sixty dental implants--20 each of external-hexagon, internal-hexagon, and Morse cone-connection designs--and their conical abutments were used in this study. Each group was subdivided into two groups of 10 loaded and 10 unloaded implants. The assemblies were immersed in human saliva and either (1) loaded with 500,000 cycles at 120 N (experimental group) or (2) incubated in static conditions for 7 days at 35°C (unloaded control group). Microorganisms were found in the internal surfaces of all types of connections. The Morse cone connection presented the lowest count of microorganisms in both the unloaded and loaded groups. Loaded implants presented with higher counts of microorganisms than unloaded implants for external- and internal-hex connections. Bacterial species from human saliva may penetrate along the implant-abutment interface under both unloaded and loaded conditions for all connections evaluated. Morse cone-connection implants showed the lowest counts of microorganisms for both conditions. External- and internal-hex implants showed a higher incidence of bacteria and higher bacterial counts after simulated loading.
  The International journal of oral & maxillofacial implants 05/2012; 27(3):551-60. · 1.78 Impact Factor
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  Available from: Maria Bernadete Riemma Pierre

  Article: In vitro characterization of chitosan gels for buccal delivery of celecoxib: influence of a penetration enhancer.

  Yara Peluso Cid, Vinícius Pedrazzi, Valéria Pereira de Sousa, Maria Bernadete Riemma Pierre
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  ABSTRACT: Celecoxib (Cx) shows high efficacy in the treatment of osteoarthritis and rheumatoid arthritis as a result of its high specificity for COX-2, without gastrolesivity or interference with platelet function at therapeutic concentrations. Besides of anti-inflammatory effects, Cx also has a potential role for oral cancer chemoprevention. For these conditions, oral administration in long-term treatment is a concern due to its systemic side effects. However, local application at the site of injury (e.g., buccal inflammation conditions or chemoprevention of oral cancer) is a promising way to reduce its toxicity. In this study, the in vitro characterization of mucoadhesive chitosan (CHT) gels associated to Azone® was assessed to explore the potential buccal mucosal administration of Cx in this tissue. Rheological properties of gels were analyzed by a rheometer with cone-plate geometry. In vitro Cx release and permeability studies used artificial membranes and pig cheek mucosa, respectively. Mucoadhesion were measured with a universal test machine. CHT gels (3.0%) containing 2.0% or 3.0% Az showed more appropriate characteristics compared to the others: pH values, rheology, higher amount of Cx retained in the mucosa, and minimal permeation through mucosa, besides the highest mucoadhesion values, ideal for buccal application. Moreover, the flux (J) and amounts of drug released decreased with increased CHT and Az concentrations. CHT gels (3.0%) associated with 2.0% or 3.0% Az may be considered potential delivery systems for buccal administration of Cx.
  AAPS PharmSciTech 12/2011; 13(1):101-11. · 1.43 Impact Factor
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  Available from: Vinícius Pedrazzi

  Article: Bacterial leakage along the implant-abutment interface: culture and DNA Checkerboard hybridization analyses.

  Cássio do Nascimento, Paola Kirsten Miani, Vinícius Pedrazzi, Kátia Muller, Rubens Ferreira de Albuquerque Junior
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  ABSTRACT: Bacterial species have been found harboring the internal surface of dental implants as consequence of their failed connections. The aim of the present study was to compare the detection frequency of bacterial leakage from human saliva through the implant-abutment interface, under non-loading conditions, using either DNA Checkerboard or culture method. Thirty dental implants with hexagonal platforms were connected to pre-machined abutments according to the manufacturers' specifications. The assemblies were individually incubated in human saliva under anaerobic conditions for 7 days at 37°C. Afterward, contents from the inner parts of the implants were collected and evaluated with either DNA Checkerboard (s = 15) or culture (n = 15). Subsequently, identification and quantitation of bacterial species from saliva and implants were carried out for the group evaluated with the DNA Checkerboard method. Both DNA Checkerboard and culture showed positive signals of bacterial leakage in 6 of the 15 evaluated samples. Capnocytophaga gingivalis and Streptococcus mutans were the most frequently detected species harboring the internal surface of the implants followed by Veillonella parvula. Occurrence of bacterial leakage along the implant-abutment interface is comparably detected with both DNA Checkerboard hybridization and conventional culture methods.
  Clinical Oral Implants Research 09/2011; 23(10):1168-72. · 2.51 Impact Factor
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  Available from: Vinícius Pedrazzi

  Article: In vitro evaluation of the microbial contamination on new toothbrushes: a preliminary study.

  Cássio do Nascimento, Thayla Tessa Scarabel, Paola Kirsten Miani, Evandro Watanabe, Vinícius Pedrazzi
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  ABSTRACT: The presence and survival of microorganisms on toothbrush bristles might play a role on the etiology of oral infections. The aim of this in vitro study was to evaluate the presence of bacterial contamination on new toothbrushes before oral contact. Forty toothbrushes from five different manufacturers were used in this experimental study. Each manufacturer was divided according to conventional local of obtaining: industry, drugstore, market, and perfumery. The toothbrush heads were completely immersed into tubes containing 5.0 mL of sterile peptonated water (dilution 1:10). A group of eight tubes containing the sterile solution was used as control. After 21 days of anaerobic incubation, occurrence of contamination was visually evaluated and confirmed by light microscopy. Bacterial growth in the medium, indicative of bristles contamination, was found in a total of 19 out of 40 samples (47.5%) evaluated: 6 out of 14 samples (42.85%) from industry group, 4 out of 8 samples (50.0%) from drugstore, 5 out of 10 samples (50.0%) from market, and 4 out of 8 samples (50.0%) from perfumery. Only the toothbrushes with bristles coated with chlorhexidine did not show contamination. The Gram-negative sporulating bacilli were the most prevalent form recovered. Except for chlorhexidine group, bacterial growth was observed in all groups evaluated irrespective local of obtaining.
  Microscopy Research and Technique 05/2011; 75(1):42-5. · 1.79 Impact Factor