Vincent Pieribone

Publications (82) View all

• Source

  Available from: Vincent Pieribone

  Dataset: Andersen, O et al. “Regulation of Iron Metabolism in the Sanguivore Lamprey Lampetra Fluviatilis--Molecular Cloning of Two Ferritin Subunits and Two Iron-Regulatory Proteins (IRP) Reveals Evolutionary Conservation of the Iron-Regulatory Element (IRE)/IRP Regulatory System.” European journal of biochemistry / FEBS 254.2 (1998): 223–229. Print.

  O Andersen, K Pantopoulos, H T Kao, M Muckenthaler, J H Youson, V Pieribone
• Conference Proceeding: Miniature voltage sensitive dye imaging system for in vivo experiments

  Joon Hyuk Park, V. Pieribone, E. Culurciello
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  ABSTRACT: Currently, there are no widely used methods of optically recording rapid electrical events over a wide cortical area in freely moving animals. This paper presents an image sensor for recording electrical activity of large regions (4-9 mm²) of the nervous tissue at high speeds (> 500 Hz) using voltage sensitive dye imaging (VSDI) in freely moving animals. Each 75 mum x 75 mum pixel consists of a photodiode of 74 mum x 34 mum and a storage capacitor of 788 fF. The image sensor has a signal-to-noise ratio of 76 dB.
  Life Science Systems and Applications Workshop, 2009. LiSSA 2009. IEEE/NIH; 05/2009
• Article: Genetically encoded fluorescent sensors of membrane potential.

  B J Baker, H Mutoh, D Dimitrov, W Akemann, A Perron, Y Iwamoto, L Jin, L B Cohen, E Y Isacoff, V A Pieribone, T Hughes, T Knöpfel
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  ABSTRACT: Imaging activity of neurons in intact brain tissue was conceived several decades ago and, after many years of development, voltage-sensitive dyes now offer the highest spatial and temporal resolution for imaging neuronal functions in the living brain. Further progress in this field is expected from the emergent development of genetically encoded fluorescent sensors of membrane potential. These fluorescent protein (FP) voltage sensors overcome the drawbacks of organic voltage sensitive dyes such as non-specificity of cell staining and the low accessibility of the dye to some cell types. In a transgenic animal, a genetically encoded sensor could in principle be expressed specifically in any cell type and would have the advantage of staining only the cell population determined by the specificity of the promoter used to drive expression. Here we critically review the current status of these developments.
  Brain Cell Biology 09/2008; 36(1-4):53-67. · 3.25 Impact Factor
• Article: Multiple messengers in descending serotonin neurons: localization and functional implications.

  T Hökfelt, U Arvidsson, S Cullheim, D Millhorn, A P Nicholas, V Pieribone, K Seroogy, B Ulfhake
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  ABSTRACT: In the present review article we summarize mainly histochemical work dealing with descending bulbospinal serotonin neurons which also express a number of neuropeptides, in particular substance P and thyrotropin releasing hormone. Such neurons have been observed both in rat, cat and monkey, and may preferentially innervate the ventral horns of the spinal cord, whereas the serotonin projections to the dorsal horn seem to lack these coexisting peptides. More recent studies indicate that a small population of medullary raphe serotonin neurons, especially at rostral levels, also synthesize the inhibitory neurotransmitter gamma-amino butyric acid (GABA). Many serotonin neurons contain the glutamate synthesizing enzyme glutaminase and can be labelled with antibodies raised against glutamate, suggesting that one and the same neuron may release several signalling substances, causing a wide spectrum of post- (and pre-) synaptic actions.
  Journal of Chemical Neuroanatomy 03/2000; 18(1-2):75-86. · 2.43 Impact Factor
• Article: Regulation of iron metabolism in the sanguivore lamprey Lampetra fluviatilis--molecular cloning of two ferritin subunits and two iron-regulatory proteins (IRP) reveals evolutionary conservation of the iron-regulatory element (IRE)/IRP regulatory system.

  O Andersen, K Pantopoulos, H T Kao, M Muckenthaler, J H Youson, V Pieribone
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  ABSTRACT: Two ferritin cDNAs were cloned from the liver and spinal cord of the sanguivore lamprey Lampetra fluviatilis, an extant representative of the ancient agnathan (jawless) stage in vertebrate evolution. The deduced proteins of 20.2 kDa (H-subunit) and 20.1 kDa (M-subunit) display 73% sequence identity, and both contain the ferroxidase center characteristic of animal H-ferritin. A highly conserved iron-responsive element (IRE) was identified in the 5' untranslated region of lamprey H-ferritin. Lamprey ferritin IRE forms a specific complex with crude lamprey and rat liver extracts, and with recombinant human iron-regulatory protein (IRP-1) in an electrophoretic mobility shift assay. Furthermore, lamprey ferritin IRE competes with labeled human ferritin IRE for binding to IRP in lamprey and mammalian extracts. Two liver cDNA sequences encoding 323 residues and 101 residues of two genetically distinct lamprey IRP were amplified by PCR. Lamprey IRP-1 and IRP-2, which are 72% identical, display about 74% sequence identity to their presumed homologues in mammals. Northern blot analysis shows that two IRP transcripts of 3.6 kb and 5.8 kb are expressed in lamprey liver. Given the ancient lineage of lampreys, the results indicate that the IRE/IRP regulatory system has remained highly conserved during the evolution of vertebrates.
  European Journal of Biochemistry 07/1998; 254(2):223-9. · 3.58 Impact Factor