Research experience
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Jan 2009–
presentResearch: Universidad Complutense de Madrid
Universidad Complutense de Madrid · Sección Departamental de Química Analítica · Heterocycles of Biological InterestSpain · Madrid
Publications (15) View all
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Article: A Fluorescent Styrylquinoline with Combined Therapeutic and Diagnostic Activities against Alzheimer’s and Prion Diseases
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ABSTRACT: (E)-6-Methyl-4′-amino-2-styrylquinoline (3) is a small molecule with the proper features to potentially diagnose, deliver therapy and monitor response to therapy in protein misfolding diseases. These features include compound fluorescent emission in the NIR region and its ability to interact with both Aβ and prion fibrils, staining them with high selectivity. Styrylquinoline 3 also inhibits Aβ self-aggregation in vitro and prion replication in the submicromolar range in a cellular context. Furthermore, it is not toxic and is able to cross the blood brain barrier in vitro (PAMPA test).ACS Medicinal Chemistry Letters 01/2013; 4:225. · 3.36 Impact Factor -
Article: Isolation and quantitative methods for analysis of non-steroidal anti-inflammatory drugs.
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ABSTRACT: Non-steroidal anti-inflammatory drugs are widespread employed in both human and animal health care to reduce ongoing inflammation, pain and fever due to their anti-inflammatory, analgesic and, antipyretic actions. Apart from the well-known biological samples, nowadays these compounds are frequently found in the environment, leading to longterm exposure resulting in adverse effects on humans and wildlife. Therefore, it is important to develop analytical methodologies to detect and control the presence of these pharmaceuticals in very different kinds of samples, from urine, serum or plasma, to river and waste water, sediments or sewage sludge, most of them having very complex matrices. Other problems to solve are the low concentration of the target analytes, the presence of a great number of potential interferences and, sometimes, incompatibilities with the detection systems. Consequently, sample pre-treatment is a very important step to take into account in the non-steroidal anti-inflammatory drugs determination. Herein we reviewed the main extraction and clean-up procedures reported in the literature for these substances: ultrasonic extraction, Soxhlet extraction, pressurized- liquid extraction, supercritical fluid extraction, microwave-assisted extraction, dispersive liquid-liquid microextraction, hollow fiber liquid phase microextraction, pressurized hot water extraction, solid-phase extraction, molecularly imprinted solid-phase extraction and solid-phase microextraction. Several analytical methodologies have been developed to quantify non-steroidal anti-inflammatory drugs, including gas chromatography-mass spectrometry, liquid chromatography with ultraviolet detection, diode array detection, fluorescence detection and tandem mass spectrometry.Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry (Formerly Cu rrent Medicinal Chemistry - Anti-Inflammatory and Anti-Allergy Agents) 08/2012; 11(1):65-95. -
Article: Fluorescence properties of the anti-tumour alkaloid luotonin A and new synthetic analogues: pH modulation as an approach to their fluorimetric quantitation in biological samples
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ABSTRACT: Luotonin A is an alkaloid structurally related to the natural anti-tumour agent camptothecin. The fluorescence behaviour of luotonin A and a series of six analogues is described in the present work. The influence of solvent polarity and pH on the native fluorescence properties of these alkaloids was studied, finding that in organic solvents or in aqueous solutions (pH 5.5–7.2) the neutral form of the luotonin derivatives emit in the region of 410–450 nm but, in both media, acidification to pH values below 3.0 causes a new emission band to appear at about 500 nm. An ESPT reaction occurs due to the protonation of the basic nitrogen atoms of the pentacyclic ring. Acid-base titrations of luotonin A and its derivatives in aqueous and acetonitrile media were carried out in order to determine their pKa n values which were around 2, showing these compounds to be very weak bases. In aqueous media, the absence of an iso-emissive point in the emission spectra suggests the existence of more than two species in the proton transfer equilibria. The basicity of the luotonin A derivatives is increased in organic media, and a good correlation between the pKa n values and the chemical structure was found. The protonation of luotonin A was also studied by 1H-NMR and 13C-NMR experiments, which proved the protonation of the nitrogen atoms at the positions 5 and 6 of the pentacyclic ring. The fluorescence quantum yields were determined in ethanol and in aqueous solutions under neutral and acidic conditions. The fluorescence quantum yields were higher in water for the case of the more polar compounds, and the opposite result was obtained for the more hydrophobic ones. The remarkable and interesting fluorescence properties of luotonin A prompted the development of its fluorimetric analytical quantitation, obtaining very good analytical features.Journal of Luminescence 02/2012; 132:2468-2475. · 2.10 Impact Factor -
Article: Miniaturized Fluorescence Assay for Studying the Effect of hydroxypropil-betacyclodextrin on the Aqueous Solubility of the Alkaloid luotonin A and Some Analogues
Luminescence 01/2012; 27:545-546. · 1.73 Impact Factor -
Article: Searching for New Fluorescent Probes to Detect Beta-Amyloid Proteins Involved in Alzheimer’s Disease
Luminescence 01/2012; 27:539-540. · 1.73 Impact Factor
