Skills (13)
Education
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Mar 2010–
Mar 2012Universidade Federal de Santa Catarina
Neurochemistry · MasterBrazil · Florianopolis -
May 2002–
Apr 2007Universidade Federal de Santa Catarina (UFSC)
Pharmacy - Clinical Analisys · BachelorBrazil · Florianópolis
Other
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LanguagesPortuguese and English.
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Scientific MembershipsSociedade Brasileira de Neurociências e Comportamento "Brazilian Society of Neurosciences and Behavior"
Questions and Answers (28) View all
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Answer added in Cell Culture5 At what percentage is DMSO considered non toxic to cells?For SH-SY5Y is recommended to use 0,1% at final concentration.
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Answer added in Immunological Methods51 About NF-kB activation in RAW264.7 (murine macrophage) cellsWhy don't you try to do an immunofluorescence or an immunocytochemistry to observe the translocation?
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Answer added in Immunofluorescence10 how I can maintain cells in culture on glass coverslips, for how long?Well, for what I understood... You have to put your coverslips in the wells of a plate, should say 12 wells plate, seed you cell normaly and waits unt... [more]
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Answer added in Genetic Engineering6 How to determine whether overexpression of Molecule A alters endogenous Molecule B expression?You could also use an siRNA for A and analyze if B diminishes.
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Answer added in Cell Signaling19 Is there a reason that the total protein measured by an antibody on a western blot test would go down, as the phosphorylated form of that protein increases?Some treatments can reduce gene expression while also induce protein phosphorilation. Have you tried to mesure mRNA expression of this receptor?
Publications (1) View all
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Article: Chronic administration of duloxetine and mirtazapine downregulates proapoptotic proteins and upregulates neurotrophin gene expression in the hippocampus and cerebral cortex of mice.
[show abstract] [hide abstract]
ABSTRACT: Structural alterations in the limbic system, neuronal cell loss, and low levels of neurotrophins have been implicated in the pathogenesis of depression. While it is generally accepted that increasing monoamine levels in the brain can effectively alleviate depression, the precise neurobiological mechanisms involved are unclear. In the present study, we examined the effects of two antidepressants, duloxetine and mirtazapine, on the expression of apoptotic and neurotrophic proteins in the cerebral cortex and hippocampus of mice. Duloxetine (10 mg/kg) and mirtazapine (3 mg/kg) were chronically administered for 21 days, and qRT-PCR analysis was carried for the following: neurotrophins (BDNF, NGF, FGF-2, and NT-3); anti-apoptotic proteins (Bcl-2 and Bcl-xL) and pro-apoptotic proteins (Bax, Bad, and p53). Both duloxetine and mirtazapine produced antidepressant activity in the forced swimming test and induced increased cortical and hippocampal mRNA expression of BDNF. Duloxetine also increased Bcl-2, Bcl-xL, FGF-2, and NT-3 expression in the cerebral cortex, and FGF-2 expression in the hippocampus. Moreover, duloxetine reduced Bax and p53 expression in the hippocampus, and Bad expression in the cerebral cortex. Mirtazapine decreased Bcl-xL and Bax expression in the hippocampus, and Bad and p53 expression in both the hippocampus and cerebral cortex. Mirtazapine also increased the expression of neurotrophins, NGF and NT-3, in the cerebral cortex. These results suggest that duloxetine and mirtazapine could elicit their therapeutic effect by modulating the activity of apoptotic and neurotrophic pathways, thus enhancing plasticity and cell survival in depressive patients.Journal of psychiatric research 03/2013; · 3.72 Impact Factor
