Topics (17) View all

Skills (48)

Research experience

    • Jan 2007–
      Dec 2012
      Research: University of Florida
      University of Florida · Department of Horticultural Sciences, Department of Environmental Horticulture, Department of Plant Molecular and Cellular Biology
      Gainesville · USA
    • Jan 2005–
      Dec 2012
      Research: University-Hospital of Padova
      University-Hospital of Padova
      Padova · Italy
    • Jan 2005–
      Dec 2012
      Research: University of Padua
      University of Padua
      Padova · Italy
  • Teaching: 6 internship students and 3 PhD students
  • Teaching: Italy 2005 Mentor: 4 final project students
  • Teaching: University of Padua
  • Teaching: Plant Physiology of Fruit Trees
  • Teaching: Teaching Assistant
  • Sep 2010–
    Sep 2011
    Research: M. euphorbiae elicitor study
    UC Riverside · Nematology · UC Riverside
    Isgouhi Kaloshian · Riverside
    aphids, tomato, tobacco, molecular biology, physiological studies.
  • Jan 2007–
    Jan 2008
    Research: Foliage colour development
    Gainesville
    anthocyanin, colour development, ornamentals, coleus.
  • May 2006–
    Sep 2010
    Research: Secondary metabolites
    UF Gainesville · Hort science · UF Gainesville
    Gainesville
    volatiles, mutants, tomato, phenyl propanoids
  • Jun 2005
    Research: Tomato QTL study with introgression lines
    UF · Hort Science · UF
    Harry Klee
    Screening of introgression lines through markers and phenotype analysis with statistical methodologies
  • Jan 2003–
    May 2006
    Research: Fruit physiology
    University of Padua · Environmental Agronomy and Crop Science · University of Padua
    Angelo Ramina Fruit tree physiology · Padova
    fruit and fruitlet abscission, fruit ripening, fruit allergenes
  • Jan 2003–
    Jan 2004
    Research: Fruit ripening and post harvest
    University of Padua · Environmental Agronomy and Plant Science · University of Padua
    Pietro Tonutti · Padova
    fruit ripening and post-harvest. Grape, peach and apple.
  • Jan 2002–
    Aug 2002
    Research: Cell Wall Metabolism
    UC Davis · Postharvest · UC Davis
    John Labavitch · Davis
    cell wall analysis, enzyme activity, ripening and post harvest disorders.
  • Sep 2001–
    Dec 2001
    Research: Red clover diversity
    IGER · Legume breeding department · IGER
    Michael Abberton and Athole Marshall legume breeding department · Aberystwyth
    AFLP, Trifolium pratense, red clover

Education

  • Sep 2003–
    Jun 2005
    Oxford school
    Certificate inFCE, Proficiency and attended Advance English
    Italy · Padova
  • Jan 2003–
    Dec 2005
    University of Padua
    Phd
    Italy · Padova
  • Feb 2001
    Progetto Euroverde
    intership
    Padova
  • Sep 1996–
    Jul 2001
    University of Padua
    Bachelor in Agricultural Sciences and Technologies
    Italy · Padova
  • Jan 1995
    ITAS GB Cerletti
    Reconstruction of a Po valley wood with indigenous species (with the supervision of prof Coradazzi)
    Italy · Conegliano
  • Jan 1995
    ITAS GB Cerletti
    Agriculture expert (Perito Agrario)
    Italy · Conegliano
  • Sep 1991
    ITAS GB Cerletti Conegliano Veneto
    Perito Agrario
    Italy · Conegliano Veneto

Awards & achievements

  • Jan 2003
    Scholarship: PHD fellowship for three years from the Italian Education Ministry
  • Jan 2002
    Scholarship: Aldo Gini foundation grant for research with Dr. John Labavitch on peach mealiness (University of California-Davis)
  • Oct 1999
    Scholarship: Leonardo da Vinci grant to work at IGER (Institute of Grassland and Environmental Research, part of the BBSRC) at Aberystwyth, UK
  • Jun 1999
    Scholarship: Aldo Gini Foundation Quality Scholarship
  • Sep 1998
    Scholarship: Erasmus Scholarship (University of Padua to study abroad at the University of Wales for 9 months
  • Sep 1996
    Scholarship: Yearly academic grant (University of Padua)

Other

  • Languages
    English, Italian, Spanish, Portoguese
  • Journal Referees
    Journal of Experimental Botany, Plant Disease, Annals of Applied Biology, New Phytologist, Australian journal of plant physiology, Transgenic Research, Etc.; a review of general semantics
  • Other Interests
    Reading, hiking, photography, especially black and white, watching the news, TV series and movies. cooking, listening to radio and music. Wine and honey tasting., The New York Times, Journal of Experimental Botany, Annals of Botany, TAG, Plant Cell, etc...., I love a lot of opublications from other people but Among mine:
    • V Dal Cin, D. M.Tieman, T. Takayuki, R. McQuinn,, R.C.H. de Vos, S. Osorio, E. Schmelz, M.G. Taylor, M. Smits-Kroon, R.C.Schuurink,.M. Haring,.J.J. Giovannoni, A. Fernie,. H.J. Klee. 2011 Ectopic expression of a MYB transcription factor defines a set of co- regulated genes involved in phenylalanineand phenylpropanoid synthesis in tomato fruit. The Plant Cell, Vol. 23: 1–17. IF 10.648.
    • V. Dal Cin, B. Kevany, Z. Fei, H. Klee 2009. Identification of Solanum habrochaites QTL that affect ethylene emissions in tomato fruit. Theoretical and Applied Genetics DOI 10.1007/s00122-009-1119-x. IF 3.264.
    • V. Dal Cin, A. Boschetti, A. Dorigoni, and A. Ramina. 2007. Benzylaminopurine application on two different apple cultivars (Malus domestica L. Borkh) displays new and unexpected fruitlet abscission features. Annals of Botany 99:1195-1202. IF 3.388.
    • V. Dal Cin, F. M. Rizzini, A. Botton and P. Tonutti. 2006. The ethylene biosynthetic and signal transduction pathways are differently affected by 1-MCP in apple and peach fruit. Postharvest Biology and Technology 42: 125–133. IF 2.256.
    • V. Dal Cin, M. Danesin, F. M. Rizzini and A. Ramina. 2005. RNA Extraction From Plant Tissues: The Use of Calcium to Precipitate Contaminating Pectic Sugars, Molecular Biotechnology 31: 113-119. IF 2.091.
    • D.A. Brummell, V. Dal Cin, S. Lurie, C.H. Crisosto, J.M. Labavitch (2004) Cell wall metabolism during the development of chilling injury in cold-stored peach fruit: association of mealiness with arrested disassembly of cell wall pectins. Journal of Experimental Botany 55, 2041-2052. IF 4.818., The Name of the Rose, Angels and Demons, La Casa de los Espiritus, Cien Anos de Soledad, The Lord of the Rings, Great Expectations, Harry Potter, Se una Notte d'Inverno un Viaggiatore, Pablo Neruda poems, A Widow for one Year, etc

Questions and Answers (20) View all

  • Answer added in Organic Synthesis
    31 Can anybody explain why there is a change in spots on TLC plate?
    By Novi Sulistyaningrum · Ministry of Health, Indonesia
    Valeriano dal cin
    I fully agree with Robert and Joseph and as a solution with Alok. Freeze it and freeze dry it. 
  • Answer added in Plasmids
    9 For how long can a plasmid be stored at -20°C?
    By John Nduko · Hokkaido University
    Valeriano dal cin
    if you do not get any colony try to change the media because the gene may be toxic but it is in the worst of the situation. I worked with one toxic pl... [more]
  • Answer added in Plasmids
    9 For how long can a plasmid be stored at -20°C?
    By John Nduko · Hokkaido University
    Valeriano dal cin
    I do not get your part John. The plasmid generally runs in three bands. The plasmid is big, if the insert is small it is almost impossible to distingu... [more]
  • Answer added in Laboratory Experiments
    7 Why does water vapor sometimes appear in agar plates?
    By Rupinder Singh · Punjabi University, Patiala
    Valeriano dal cin
    Neveen got it! do not overdry them and store them upside down in refrigerator. 
  • Answer added in Plasmids
    9 For how long can a plasmid be stored at -20°C?
    By John Nduko · Hokkaido University
    Valeriano dal cin
    it really depends on the plasmid type and plasmid structure, better would be storing at -80C. Supercoiled are more stable to my knowledge....but....It... [more]

Publications (20) View all

  • Article: In Planta Expression or Delivery of Potato Aphid Macrosiphum euphorbiae Effectors Me10 and Me23 Enhances Aphid Fecundity.
    Hagop S Atamian, Ritu Chaudhary, Valeriano Dal Cin, Ergude Bao, Thomas Girke, Isgouhi Kaloshian
    [show abstract] [hide abstract]
    ABSTRACT: The interactions between aphids and their host plants seem to be analogous to those of plant-microbial pathogens. Unlike microbial pathogen effectors, little is known about aphid effectors and their ability to interfere with host immunity. To date, only three functional aphid effectors have been reported. To identify potato aphid (Macrosiphum euphorbiae) effectors, we developed a salivary gland transcriptome using Illumina technology. We generated 85 million Illumina reads from salivary glands and assembled them into 646 contigs. Ab initio sequence analysis predicted secretion signal peptides in 24% of these sequences, suggesting that they might be secreted into the plant during aphid feeding. Eight of these candidate effectors with secretion signal peptides were functionally characterized using Agrobacterium tumefaciens-mediated transient overexpression in Nicotiana benthamiana. Two candidate effectors, Me10 and Me23, increased aphid fecundity, suggesting their ability to suppress N. benthamiana defenses. Five of these candidate effectors, including Me10 and Me23, were also analyzed in tomato by delivering them through the Pseudomonas syringae type three secretion system. In tomato, only Me10 increased aphid fecundity. This work identified two additional aphid effectors with ability to manipulate the host for their advantage.
    Molecular Plant-Microbe Interactions 01/2013; 26(1):67-74. · 4.43 Impact Factor
  • Article: Ethylene and preharvest drop: the effect of AVG and NAA on fruit abscission in apple (Malus domestica L. Borkh)
    Valeriano Dal Cin, Marcello Danesin, Alessandro Botton, Andrea Boschetti, Alberto Dorigoni, Angelo Ramina
    [show abstract] [hide abstract]
    ABSTRACT: l-Aminoethoxyvinylglycine (AVG) and 1-naphthylacetic acid (NAA) are known to affect preharvest fruit drop, fruit quality and fruit maturation in Golden Delicious apples (Malus×domestica Borkh). Experiments were carried out on GD/M9 trees treated at three different developmental stages (41, 28 and 17days before the beginning of the commercial harvest) with AVG and NAA. Both chemicals significantly reduced fruit drop without significantly affecting the fruit weight. Background colour development and ripening were both delayed by AVG, whereas NAA significantly enhanced yellowing without affecting the evolution of ripening. Ethylene evolution and transcription profiles of genes involved in ethylene biosynthesis (MdACS1 and MdACO1) and action (MdETR1, MdERS1 and MdCTR1) were monitored in cortex from the date of the first treatment until the beginning of fruit drop in the control trees (21days after the beginning of commercial harvest). AVG blocked or efficiently reduced the ethylene evolution. This effect was paralleled by a down-regulation of MdACS1, MdACO1, MdETR1 and MdERS1. NAA at the second and third date of application enhanced the onset of ethylene evolution, although, at the end of the experiment, no difference was found between control and treated fruits. The chemical applied in the first date significantly down-regulated the transcription of the genes at the end of the experiment. MdCTR1 expression, basically unaffected by AVG and NAA, appeared to be transiently down-regulated. The initial down-regulation is under developmental control, whereas the late regain of transcript accumulation paralleled the ethylene evolution.
    Plant Growth Regulation 04/2012; 56(3):317-325. · 1.60 Impact Factor
  • Article: MdACO expression during abscission
    Valeriano Dal Cin, Giulio Galla, Angelo Ramina
    [show abstract] [hide abstract]
    ABSTRACT: The last step of ethylene biosynthesis in apple is catalyzed by ACO (1-aminocyclopropane 1-carboxylic acid oxidase) encoded by MdACO1 and MdACO2. Both genes were expressed during early fruit development although at different level. Later on, the ACO transcript accumulation in persisting fruitlets decreased whereas in fruitlets undergoing abscission MdACO2 transcripts remained constant and those of MdACO1dramatically increased. The expression of MdACO was assessed by a particular type of semi-quantitative PCR involving 33P labeled primers and the following electrophoresis in polyacrylamide gel. The use of either the reverse primer or the anchored oligo dT13 produced clear and elegant results. Nevertheless, the initial quantity of labeled primer appeared to be a crucial factor for obtaining a wide range titration curve.
    Molecular Biotechnology 04/2012; 36(1):9-13. · 2.17 Impact Factor
  • Article: RNA extraction from plant tissues
    Valeriano Dal Cin, Marcello Danesin, Fabio Massimo Rizzini, Angelo Ramina
    [show abstract] [hide abstract]
    ABSTRACT: Several protocols and commercial kits are used for the extraction of nucleic acids from different plant tissues. Although there are several procedures available to remove sugars, which hinder the extraction of clean genomic DNA, there are few to assist with extraction of RNA. Those presently used include precipitations with ethylene glycol monobutyl ether or lithium chloride (LiCl), or centrifugation in cesium chloride (CsCl) gradients, but these generally either do not allow high recovery of RNA, are time consuming, rely on hazardous chemicals or need special equipment. Here we present the use of the simple cation, Ca2+, which has been tested and shown to be very efficient for the precipitation of high molecular weight pectic sugars during RNA extraction. Results are presented for different plant tissues, especially tissues of peach and apple fruits at varying ripening stages.
    Molecular Biotechnology 04/2012; 31(2):113-119. · 2.17 Impact Factor
  • Article: Identification of genes in the phenylalanine metabolic pathway by ectopic expression of a MYB transcription factor in tomato fruit.
    Valeriano Dal Cin, Denise M Tieman, Takayuki Tohge, Ryan McQuinn, Ric C H de Vos, Sonia Osorio, Eric A Schmelz, Mark G Taylor, Miriam T Smits-Kroon, Robert C Schuurink, Michel A Haring, James Giovannoni, Alisdair R Fernie, Harry J Klee
    [show abstract] [hide abstract]
    ABSTRACT: Altering expression of transcription factors can be an effective means to coordinately modulate entire metabolic pathways in plants. It can also provide useful information concerning the identities of genes that constitute metabolic networks. Here, we used ectopic expression of a MYB transcription factor, Petunia hybrida ODORANT1, to alter Phe and phenylpropanoid metabolism in tomato (Solanum lycopersicum) fruits. Despite the importance of Phe and phenylpropanoids to plant and human health, the pathway for Phe synthesis has not been unambiguously determined. Microarray analysis of ripening fruits from transgenic and control plants permitted identification of a suite of coregulated genes involved in synthesis and further metabolism of Phe. The pattern of coregulated gene expression facilitated discovery of the tomato gene encoding prephenate aminotransferase, which converts prephenate to arogenate. The expression and biochemical data establish an arogenate pathway for Phe synthesis in tomato fruits. Metabolic profiling and ¹³C flux analysis of ripe fruits further revealed large increases in the levels of a specific subset of phenylpropanoid compounds. However, while increased levels of these human nutrition-related phenylpropanoids may be desirable, there were no increases in levels of Phe-derived flavor volatiles.
    The Plant Cell 07/2011; 23(7):2738-53. · 8.99 Impact Factor

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