Titik Budiati

PhD and M.Sc. (Food Safety), M.Eng. (Indust. Eng)
Lecturer and Researcher
politeknik negeri jember · Food Technology Department

Publications

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    ABSTRACT: In this study, two preparation sample (pelleted and non-pelleted) and two pre-enrichment (with and without pre-enrichment in Half Frazer Broth) were assessed on their efficacy to isolate Listeria from naturally contaminated catfish and tilapia samples. A total of 108 samples (32 catfish intestines, 32 tilapia intestines, and 44 water samples) were examined for the presence of Listeria. A total of 108 samples (32 catfish intestines, 32 tilapia intestines, and 44 water samples) were examined. Out of 16 Listeria were observed on pelleted sample and 5 Listeria were observed on non-pelleted sample. All samples were preceded without pre-enrichment. Nineteen (19) isolates were observed on pelleted sample 17 on non-pelleted sample. Those were combined with pre-enrichment. The study revealed that, the isolation of Listeria species by pelleting the sample in the combination with pre-enrichment from naturally contaminated catfish, tilapia and water samples were relatively higher than their opponents. By improving the efficacy of isolating Listeria is beneficial for reporting the presence of these pathogenic bacteria due to public health purposes.
    Emirates Journal of Food and Agriculture. 08/2013; 25(8).
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    ABSTRACT: The objectives of the study were to determine the prevalence, antibiotic resistance and occurrence of plasmids in Salmonella isolated from catfish (Clarias gariepinus) and tilapia (Tilapia mossambica) obtained from wet markets and ponds in Malaysia. A total of 172 samples (32 catfish carcass rinse, 32 catfish intestines, 32 tilapia carcass rinse, 32 tilapia intestines, and 44 water samples) were obtained from nine wet markets and eight ponds that were fed chicken offals, spoiled eggs, and commercial fish feed from 2008 to 2009. Seven Salmonella serovars were isolated from 9/32 catfish (28.1%), 14/32 tilapia (43.8%), and 11/44 (25%) water samples. These include S. Albany, S. Agona, S. Corvallis, S. Stanley, S. Typhimurium, S. Mikawashima and S. Bovis-mobificans. Salmonella isolates were resistant to chloramphenicol (C, 37.2%), clindamycin (Da, 100%), rifampicin (Rd, 90.7%), spectinomycin (Sh, 27.9%), and tetracycline (Te, 67.4%). The multiple antibiotic resistance index of Salmonella isolates ranged from 0.32 to 0.45 for catfish; 0.14 to 0.36 for tilapia; and 0.27 to 0.36 for water. The predominant antimicrobial resistance profiles of Salmonella serovars from catfish, tilapia and water were CDaRdTe (4/13), DaRdSh (4/19), and DaRdTe (6/11), respectively. The plasmids of Salmonella serovars isolated from catfish ranged from 23 to 80 kb; those for tilapia ranged from 6 to 90 kb; that for water ranged from 6 to 70 kb, respectively. The presence of plasmids represents a potential health hazard since plasmids can mediate the transfer of antibiotic resistance genes to other bacteria present in the fish, and aquaculture environment, which can also enter the food chain.
    Aquaculture 01/2013; 372-375:127-132. · 2.01 Impact Factor
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    ABSTRACT: Monitoring in the anaerobic bioreactor system is requiring understanding the occurred situation in the bioreactor process. Bioreactor is complex designed to accelerate waste degradation by combining attributes of the aerobic and anaerobic bioreactors involves many variables. Multivariate Statistical Process Control (MSPC) models are a statistical solution to the problem of directly calculating physical and biological properties of molecules from their physical structure. QSAR model is utilized to extract information from a set of numerical descriptors characterizing molecular structure and use this information to develop inductively a relationship between structure and property. The goal of a (MSPC) model is to replace the conventional methods univariate Statistical Process Control (SPC) to analyze the state of the multivariate process of anaerobic bioreactor. The objective of the sequential aerobic-anaerobic treatment is to cause the rapid biodegradation of degradable waste in the aerobic stage in order to reduce the production of organic acids in the anaerobic stage resulting in the earlier onset of methanogenesis. The monitoring of process uses principal component analysis (PCA) to reduce multivariate data. Further, hotelling T² values were used to monitor the quality of the bioreactor operating condition. Hence, fuzzy logic was used to determine the present condition of the bioreactor based on the value of T² related. The simulation results indicate that the offered method is able to determine four bioreactor process states, i.e. normal, organic overload, hydraulic overload, and fluctuations in temperature, with the success rate 100%.
    Journal of Engineering Science and Technology 01/2012; 7(1):56-72.
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    ABSTRACT: A Neural Network Internal Model Control (NN- IMC) strategy is investigated, by establishing inverse and forward model based neural network (NN). Further for developing the model has been selected suitable adaptive filter. Two types of NN-based inverse model (i.e. with and without disturbance input) were accurately simulated. The results indicated that the neural networks are capable to establish forward and inverse model rapidly from the couple of input-output open loop data of single distillation column binary system with a good root mean square error (RMSE). The simulation results revealed that NN-IMC with appropriate learning rate - momentum is capable to pursue the set-point changes and to reject the disturbance changes without steady state error or oscillations. NN-IMC with inverse model which contains disturbance input (modified NN-IMC) offer better performance than without it (conventional NN-IMC).
    International Journal of Engineering, Science and Technology. 01/2010;
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    Budiati Titik
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    ABSTRACT: Catfish is a one of freshwater fish which has good nutrition for human but it can be contaminated by antibiotic such as fluoroquinolones. In risk assessment , the no-observed-effect concentration (NOEC) of fluoroquinolones was considered to be 0.0080 g/kg/day and acceptable daily intake (ADI) for fluoroquinolone is 0.15 μg/kg/day on the basis body weight. There are no reports of toxicity in man or animals from the consumption of fluoroquionolones in catfish and the levels of fluoroquinolones in catfish were very low, most often at or near the minimum level of detection (5.0 ppb or 5.0 g/kg). Therefore, fluoroquinolones from catfish consumption isnot considered to represent a significant health risk.
    International Journal of Engineering Science and Technology. 01/2010;
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    Titik Budiati, Naiyana
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    ABSTRACT: The objectives of this study were predicting the transmission and survival of L. monocytogenes in cooked ham during supply chain. Cooked ham are frequently contaminated with L. monocytogenes during postprocessing steps through contact on surface of processing, handling, packaging equipment. Transfer rate of L. monocytogenes on static and dynamic condition in various surface type was investigated. The prevalence and level of L. monocytogenes in cooked ham at plant as well as the prevalence of unsatisfactory processing at retail were studied. A Monte Carlo simulation model was created by using @risk. The simulation predicted that the prevalence was 11.76 % with 90% confidence interval of 2% to 25% and estimated level was -4.02 log CFU/ cm2 . It was estimated to be occurred on slicing step at plant. Our results suggest that, the prevalence and level of L. monocytogenes can be reduced by Good Handling Process application and/or HACCP application
    International Food Research Journal 01/2010; 17:267-280.
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    Titik Budiati
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    ABSTRACT: Salmon is a kind of fish which has good nutrition for human but it can be contaminated by heavy metal such as arsenic. In risk assessment , the provisional tolerable weekly intake (PTWI) for inorganic arsenic is 15 μg/kg b.w./week and the organic forms of arsenic present in sea foods need different consideration from the inorganic arsenic in water. There are no reports of toxicity in man or animals from the consumption of organoarsenicals in seafood. Organic arsenic compounds such as arsenobetaine and arsenocholine seem not to be converted to inorganic arsenic in vivo and not genotoxic in mammalian cells in vitro. Therefore, arsenobetaine and arsenocholine from fish and sea food consumption is not considered to represent a significant health risk.
    Australian Journal of Basic and Applied Sciences. 01/2010; 10(5):6-12.

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