Tianrong Cheng
Ph.D.
HuaBao International Holdings

Publications
Sorted by: Published

  • Article: Fluorescent detection of lipid peroxidation derived protein adducts upon in-vitro cigarette smoke exposure.
    R Patrick Rainey, I Gene Gillman, Xuling Shi, Tianrong Cheng, Andy Stinson, Diana Gietl, Anthony P Albino
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    ABSTRACT: Oxidative stress in biological systems can result in radical-induced lipid peroxidation (LPO), which can lead to the production of secondary reactive by-products such as 4-hydroxy-2-nonenal (HNE), malondialdehyde (MDA), acrolein, and acetaldehyde. These deleterious compounds are known to react with and concomitantly modify nucleophilic amino acid residues on proteins. Oxidative stress induced by cigarette smoke (CS) has been put forth as a major mechanism for tobacco-induced pathologies. At present, there are few reliable biomarkers for measuring the extent of oxidatively-induced damage resulting from CS exposure in vivo. This study has utilized a previously reported CS exposure system to expose cultured cells in-vitro to whole CS and determine the extent of LPO resulting from CS exposure by quantifying the increase in HNE within the exposure media versus controls via gas chromatography mass spectrometry. Additionally, we obtained protein enriched cell lysate post-CS exposures and measured the fluorescent signal obtained via direct injection fluorescent analysis at 375 nm ex./415 nm em. This study determined that the fluorescent signal intensity was directly proportional to the quantity increase of HNE in CS exposed media. It further tested this correlation by performing HNE titration addition experiments to cultured cells and Western blot analysis on proteins obtained from cell lysates. Finally, the fluorescent signal increase from authentic BSA solutions incubated with increasing concentrations of HNE was measured. It is proposed that the fluorescent signal observed from the protein lysate of CS exposed cultured cells corresponds to the extent of biological damage resulting from secondary reactive by-products formed from LPO induced via CS exposure and represented by HNE. The fluorescent signals increased in intensity upon increasing CS dose up to 20 min and remained elevated over 24 h after cessation of CS exposure.
    Toxicology mechanisms and methods 09/2009; 19(6-7):401-9. · 1.03 Impact Factor
  • Article: Photophysical Studies on the Mono- and Dichromophoric Hemicyanine Dyes III. Ultrafast Fluorescence Up-conversion in Methanol:  Twisting Intramolecular Charge Transfer and “Two-State Three-Mode” Model
    Yanyi Huang, Tianrong Cheng, Fuyou Li, Chun-Hui Huang
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    ABSTRACT: A series of hemicyanine dyes, including monomer ([(E)-N-methyl-4-(2-(4-N,N-dimethyl phenyl) ethenyl) pyridinium] iodide; M) and dimers (1,n-Bis [(E)- 4-(2-(4-N,N-dimethyl phenyl) ethenyl) pyridinyl]-alkane dibromide; n = 3, alkane = propane, B3; n = 5, alkane = pentane, B5; n = 12, alkane = dodecane, B12), are synthesized and their ultrafast fluorescence up-conversion behaviors are studied. The fluorescence decay curves of these dyes can be well fitted by the sum of several exponential decays in the shorter wavelengths and by the sum of the exponential rise(s) with decay(s) in the longer wavelengths. The fact that the values of longest lifetime component at longer wavelengths tend to be a constant proves the existence of a “sink” region on the potential energy surface of excited state. This sink region is around the TICT state, by which the nonradiative transition dominates the deactive decay paths of excited state. The time dependent Stokes shift function analysis of these four dye molecules in methanol indicates that the B5's unique “solvation” behavior is different from others. This difference is contributed by the increased difficulty of the TICT formation of B5 originated from its folded conformation. The time dependent Stokes shifts of M, B3, and B12 are due to not only solvation dynamics but also TICT formation. Detailed analysis is presented in the frame of a “two-state three-mode” model.
    The Journal of Physical Chemistry B 01/2002; 106(39):10041-10050. · 3.70 Impact Factor
  • Source
    Article: Photophysical Studies on the Mono- and Dichromophoric Hemicyanine Dyes II. Solvent Effects and Dynamic Fluorescence Spectra Study in Chloroform and in LB Films
    Yanyi Huang, Tianrong Cheng, Fuyou Li, Chuping Luo, Chun-Hui Huang
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    ABSTRACT: A series of hemicyanine dimers, (B3, B5, and B12) whose two chromophores are linked by different numbers of methylene groups, are synthesized. Negative slovatochromic behaviors are observed, and the fluorescence quantum yield is sensitive to the polarity of solvent. In chloroform, the fluorescence intensity is much more intensive than in other polar solvents, and the decay lifetime is much longer. This fluorescence sensitivity to solvent is due to the twisted intramolecular charge transfer (TICT) state formation, which can be blocked in weak polar solvent. The two chromophores in one dimer molecule are correlated and the TICT formation of dimer is relatively difficult compared with monomer. When the linkage is long enough, this correlation can be reduced. The dynamic fluorescence spectra of the chloroform solutions of these dyes support our assumption. The time-resolved fluorescence studies of their LB films provide a new perspective on the excited-state deactive process. The two-dimensional Förster energy transfer between the chromphores within monolayer is assumed to play an important role of their excited states in these LB samples. The difference of the proportions of energy transfer is supposed to be the responsible for the difference of their photoelectro conversion efficiencies.
    The Journal of Physical Chemistry B 01/2002; 106(39):10031-10040. · 3.70 Impact Factor
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    Article: Photophysical Studies on the Mono- and Dichromophoric Hemicyanine Dyes I. Photoelectric Conversion from the Dye Modified ITO Electrodes
    Yanyi Huang, Tianrong Cheng, Fuyou Li, Chun-Hui Huang
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    ABSTRACT: Stilbazolium dimers were designed and synthesized in which methylene groups of different lengths link the two chromophores. The second-order nonlinear optical property has been detected from their Langmuir−Blodgett monolayers. Photocurrent generation as well as other photophysical properties such as absorption and fluorescence were investigated. Comparing the dimers with the stilbazolium monomer, we found strong second harmonic generation (SHG) signals and remarkable enhanced photoelectric conversion (PEC) quantum yields from 1,3-Bis [(E)- 4-(2-(4-(N-methyl, N-octadecylamino) phenyl) ethenyl) pyridinyl]-alkane dibromide (B3) and 1,5-Bis [(E)- 4-(2-(4-(N-methyl, N-octadecylamino) phenyl) ethenyl) pyridinyl]-alkane dibromide (B5). The structures of the Langmuir−Blodgett films of these dyes are assumed. Although these dimers are center-symmetric (or quasi-center-symmetric) molecules, they can form the ordered non-center-symmetric (in normal direction) monolayer by the compress force and the molecular interaction between the amphiphilic dye molecules and the water molecules at the air/water interface. The influences of bias, donor/acceptor, soluble oxygen, and light intensity to photocurrent generation were studied; our results provide a new perspective to improve the performance of functional molecular material without any change of the chemical structure of the chromophore itself.
    The Journal of Physical Chemistry B 01/2002; 106(39):10020-10030. · 3.70 Impact Factor
  • Article: A Study of Aluminum-Substituted Iron Dextran Complexes by Mössbauer Spectroscopy and X-ray Diffraction
    Tianrong Cheng, † Robert Bereman, † Eddy De Grave, ‡§, Larry H. Bowen
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    ABSTRACT: Two aluminum-substituted iron dextran complexes and one nonsubstituted have been synthesized and characterized by Mössbauer spectroscopy over a wide temperature range (10−298 K) and by X-ray powder diffraction at room temperature. The mean coherence lengths in the directions perpendicular to (211) and (200) are different, indicating that the core iron oxide materials are oblong in shape. The X-ray diffraction patterns of all complexes are very similar, with broad, weak peaks at 5.0, 3.3, 2.5, 2.25, 1.6, and 1.8 Å. These values are consistent with cell-contracted akaganeite, β-FeOOH. The three samples exhibit different temperatures for the onset of magnetic splitting in their Mössbauer spectra. The Mössbauer spectra obtained at room temperature can be adequately fitted with a model-independent distribution of quadrupole doublets, while at low temperatures a superposition of distributions of sextets and doublets is required. The obtained Mössbauer parameters are in line with the structural features. This is the first report on nanoscale aluminum-substituted iron oxides as a component of iron dextran.
    Chemistry of Materials 01/2001; 13(1):136-140. · 7.29 Impact Factor
  • Article: Phase transition of lipid-like monolayer characterized by second harmonic generation
    Anchi Yu, Qing Chang, Xinsheng Zhao, Qingzhong Zhou, Dong Li, Yanyi Huang, Tianrong Cheng, Chunhui Huang
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    ABSTRACT: Phase transition of a lipid-like hemicyanine compound characterized by second harmonic generation is studied carefully. The phase transition is assigned as the first order transition between solid state and liquid state. The transition temperature increases with an increase in the surface molecular concentration. A monolayer structure parametera which is very sensitive to the phase transition is introduced.
    Science in China Series B Chemistry 04/1999; 42(2):210-216. · 1.20 Impact Factor
  • Article: 利用飞秒荧光上转换方法研究芪唑盐的激发态转动异构动力学
    Shufeng Wang, Wentao Huang, Qihuang Gong, Yanyi Huang, Tianrong Cheng, Chunhui Huang
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    ABSTRACT: 飞秒荧光上转换是一种探测分子超快荧光动力学的新方法．其原理是利用具有大的二阶非线性系数的晶体（如ＫＤＰ和ＢＢＯ）的和频作用，将飞秒光激发产生的荧光与飞秒基频激光进行和频．通过改变基频光与荧光的相对光程差，可以获得飞秒分辨的荧光衰减动力学图像．
    Chinese Journal of Quantum Electronics. 01/1999; 16(6).
  • Article: 氮杂芪唑盐单层LB膜修饰的ITO玻璃电极上的光电响应研究
    Tianrong Cheng, Chunhui Huang, Liangbin Gan
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    ABSTRACT: 近年来,我们发现一些染料分子表现出了多性能性,如Squaraine,它不仅具有相当好的非线性光学性质,而且因具有好的光电性质已成为材料进入应用,我们在研究多种染料体系中同时发现了它们优良的二阶非线性光学性质和光电化学性质,本文研究的氮杂芪唑盐是专为研究分子的二阶非线性活性理论而设计的一个化合物,它具有比Squaraine更好的二次谐波产生能力,我们对其作为光电材料的光电化学性质进行了研究,并就其光电转换的可能机理进行了探讨,在仿生材料中,LB膜方法是一种极为恰当和有效的方法,它有望克服无机固体器件的体积限制而产生新的功能.
    Chinese Science Bulletin 01/1997; 42(10):1065-1069. · 1.32 Impact Factor
  • Article: Investigation of the photoelectric conversion of a novel molecule(E)-N-methyl-4-{2-[4-(dihexadecylamino)phenyl]ethenyl}pyridazinium iodide, inLB films fabricated on an SnO2 electrode
    T-R. Cheng, C-H. Huang, L-B. Gan
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    ABSTRACT: An investigation of cathodic photocurrent response from a novel molecule with strong non-linear optical properties (second harmonic generation), (E)-N -methyl-4-{2-[4-(dihexadecylamino)phenyl]ethenyl}pyridazinium iodide (MHPd) in LB films on transparent SnO 2 glass has been carried out. A quantum yield of 0.3% was obtained under irradiation with 450 nm light in 0.5 mol dm -3 KCl electrolyte solution and ambient conditions. The studies of the relationship between the light intensity and the cathodic photocurrent indicate that MHPd assemblies perform a unimolecular process in charge recombination. The effects of bias voltage, oxygen and nitrogen, electron donors and acceptors such as methyl viologen diiodide, ascorbic acid and hydroquinone were examined and have provided supporting evidence for the proposed electron-transfer mechanism.
    Journal of Materials Chemistry 01/1997; 7:631-635. · 5.97 Impact Factor
  • Article: Second-order non-linear optical properties of Langmuir–Blodgett films of a new azo compound(p-NO2)C6H4N NC10H6O(CH2)3N(CH3)2C18H37Br and its europium complex
    W. S. Xia, C. H. Huang, T. R. Cheng, L. B. Gan, X. S. Zhao, A. C. Yu
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    ABSTRACT: A new azo compound, N,N,N-dimethyloctadecyl-3-{[4-(4-nitrophenylazo)]-l-naphthoxy}propyl quarternary ammonium bromide and its europium complex have been synthesized and their second-order non-linear optical properties have been investigated. The molecular hyperpolarizabilities, β were evaluated to be 2.55 × 10–28 and 2.95 × 10–28 esu, respectively. Data show that both compounds have good film formation properties. A quadratic relationship between the SHG signal and the number of layers was obtained in the complex with a large rare-earth-metal complex anion. These novel compounds provide new possibilities for designing high-quantity second-order non-linear optical materials to solve the quadratic SHG enhancement in LB films.
    Journal of Materials Chemistry 01/1996; 6:1493-1495. · 5.97 Impact Factor
  • Article: Effects of Free Radicals on the Collagen Type Change in Cartilage Matrix
    Zhang, Fa-hao, Cheng, Tian-rong, Chang, Shu-yun, Xu, Shan-jin, Wang, Kui
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    ABSTRACT: In this paper, a method of determining the percentage of type Ⅱ collagen in extracellular matrix of cultured chondrocytes or caritilage tissue in terms of the spectrophotometric densities of specific cyangen bromide peptide bonds on SDS-polyacrylamide gels has been used. The results show that the free radical generating systems, including Fe(Ⅱ)-EDTA,Xanthine Oxidase-Xanthine. Fulvic acid and Fusarium toxin, can cause chondrocytes to synthesize and secrete mainly type Ⅰ collagen instead of the normal type Ⅱ collagen. In addition, The collagen type change can be inhibited by Se(Ⅳ) compounds.
    Chinese Journal of Biochemistry and Molecular Biology. 01/1994; 10(02):155-158.
  • Article: Free radicals-induced abnormal chondrocytes, matrix and mineralization.A new concept of Kaschin-Beck's disease.
    K Wang, S J Xu, F H Zhang, C X Wang, S Y Chang, J Y Zhang, J X Zhang, T R Cheng
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    ABSTRACT: Free radical generating substances, including fulvic acids, mycotoxins, Fe(II), etc attack chondrocytes, causing dedifferentiation. The dedifferentiated cells synthesize and secrete abnormal collagens rich in type I instead of the normal type II. These substances also directly attack collagens in the extracellular matrix. The abnormal collagens make the matrix change from hydroxyapatite crystallization inhibiting to promoting, and give crystals of low crystallinity, high aggregation and small size. This study leads to a new concept of the development of Kaschin-Beck's Disease (KBD).
    Chinese medical journal 05/1991; 104(4):307-12. · 0.86 Impact Factor
  • Article: ABNORMAL CHONDROCYTE, MATRIX AND ABNORMAL MINERALIZATION INDUCED BY FREE RADICAL
    Kui Wang, Shanjin Xu, Fahao Zhang, Chunxia Wang, Tianrong Cheng, Shuyun Chang, Lan Wu, Jingxia Zhang, Zhenghong Yang, Yaxian Su
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    ABSTRACT: In this paper.the effects of free radical and FA on chondrocyte have been studied.The result Shows that free radicals and FA can damage chondrocytes and the damage chondrocyte secertes abnormal matrix.In the adnonmal matrix, the mineralization of hydrxoaptite is also abnormal. Based on these results, authors have point out a chemial process of KBD pathology.
    Chinese Journal of Control of Endemic Disease. 01/1990; 5(1):5-8.
  • Article: 亚硝酸钠的毒害及解救
    Tianrong Cheng
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    ABSTRACT: 亚硝酸钠是一种用途广泛,但人体摄入过量后能引起严重毒害的物质.亚硝酸钠有强氧化性,能使体内正常的血红蛋白中的两价铁离子氧化成三价铁离子,从而使血红蛋白失去可逆性运送氧气和交换二氧化碳的能力.亚硝酸钠中毒后常出现严重缺氧症状.急救时一般采用洗胃、注射亚甲兰或Vc等措施.这里同样利用氧化还原的原理,即这些药物本身极易氧化,而使三价铁离子还原成两价铁离子,从而恢复血红蛋白的正常生理功能.当然,严重时则需输血,以补充新鲜的血红蛋白.
    Chinese Journal of Chemical education. 01/1989; 2(1-2):39.
  • Article: Inhibition of Crystal Formation and Aggregation of Calcium Bilirubinate with Disodium 1-Hydroxyethanediphosphonate(EHDP)
    Wang Kui, Xu Shanjin, Hu Jiaping, Cheng Tianrong, Jin Fan
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    ABSTRACT: Disodium 1-hydroxyethanediphosphoriate was studied as an inhibitor of the formation and aggregation of calcium bilirubinate, the main component of pigment gallstone.The kinetics of calcium bilirubinate formation was studied at pH 7.9, 37+1℃, under nitrogen atmosphere, in the darkness. EHDPwas added in different levels, with or without choric acid. It is obvious that EHDPis able to inhibit the precipitation of calcium bilirubinate and the coexisted choric acid enhances this effect.The effect of EHDPon the particle size was also studied with Coulter counter. The results show that EHDPinhibit the aggregation effectively and a strong synergic effect was observed between EHDP and choric acid.
    Chemical Journal of Chinese Universities -Chinese Edition- 01/1988; 9(12):1207-1210. · 0.62 Impact Factor