Tatiana Dinarieva

The study describes the effect of aerobic conditions on the proteome of facultative homofermentative lactic acid bacterium Lacticaseibacillus rhamnosus CM MSU 529 grown in a batch culture. Aeration caused the induction of the biosynthesis of 43 proteins, while 14 proteins were downregulated as detected by label-free LC-MS/MS. Upregulated proteins a...

Lacticaseibacillus rhamnosus CM MSU 529 was grown in a batch culture under intensive aeration in the presence of 38 mkM hemin and 18 mkM vitamin K2 as a source of menaquinone to activate respiratory metabolism. Unsupplemented aerobic culture served as a control. Supplementation of the growth mediumwith hemin or menaquinone alone had no significant...

Two strains of sulfate-reducing bacteria of the genus Desulfovibrio (A2 and TomC) isolated from metal mining waste were able to grow on agar Postgate C nutrient medium under microaerobic conditions. Since their growth in liquid nutrient medium was just slightly affected by 1% O2 (initial concentration in the gas phase) and 0.05-0.1 mM H2O2, these s...

Methylamine dehydrogenase (MADH) and azurin were purified from the periplasmic fraction of the methylamine-grown obligate methylotroph Methylobacillus flagellatus KT. The molecular mass of the purified azurin was 16.3 kDa, as measured by SDS-PAGE, or 13 920 Da as determined by MALDI-TOF mass spectrometry. Azurin of M. flagellatus KT contained 1 cop...

The iron-oxidizing system of a moderately thermophilic, extremely acidophilic, gram-positive mixotroph, Sulfobacillus sibiricus N1(T), was studied by spectroscopic, high-performance liquid chromatography and inhibitory analyses. Hemes B, A, and O were detected in membranes of S. sibiricus N1(T). It is proposed that the electron transport chain from...

Cells of the Gram-negative obligate methylotrophic bacterium Methylobacillus flagellation KT, which can only use methanol and methylamine as sole carbon and energy source, oxidize 1,2-propanediol to lactaldehyde and lactate by using a pyrroloquinoline quinone (PQQ) containing cytochrome c dependent methanol dehydrogenase and an NAD+-dependent aldeh...

The cbo-type oxidase of Methylobacillus flagellatus KT was purified to homogeneity by preparative native gel electrophoresis, and the kinetic properties and substrate specificity of the enzyme were studied. Ascorbate and ascorbate/N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) were oxidized by cytochrome cbo with a pH optimum of 8.3. With TMPD as...

The cbo-type oxidase of Methylobacillus flagellatus KT was purified to homogeneity by preparative native gel electrophoresis, and the kinetic properties and substrate specificity of the enzyme were studied. Ascorbate and ascorbate/N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) were oxidized by cytochrome cbo with a pH optimum of 8.3. With TMPD as an...

Membranes of the obligate methylotroph Methylobacillus flagellatus KT contained hemes B, O, and C and cytochromes b, o, and c both in batch and in continuous cultures. Neither heme A nor heme D was detected in the membranes. The cytochromes o and bb were the main components reversibly binding carbon monoxide (CO) in the terminal part of the respira...

The o-type oxidase from the methanol-grown obligate methylotroph Methylobacillus flagellatus KT has been purified to homogeneity. The complex is composed of four subunits (57, 40, 35 and 30 kDa). It contains six haems (4C:1B:1O) and one copper atom per molecule. It is proposed that the haem O-Cu(B) binuclear centre and a low-spin haem B are located...

A method was developed to study the biodegradation and oxidative biodehalogenation of fluorinated phenols by 19F nuclear magnetic resonance (NMR). Characterization of the 19F NMR spectra of metabolite profiles of a series of fluorophenols, converted by purified phenol hydroxylase, catechol 1,2-dioxygenase, and/or by the yeast-like fungus Exophiala...

Raising the copper concentration from 0.04 to 1 mg/1 in a batch culture of the obligate methylotroph Methylobacillus flagellatum KT grown on methylamine led to the induction of amycyanine synthesis. The appearance of an additional electron carrier in the respiratory chain enhanced the oxidase activity of both whole cells and their membrane preparat...

Two oxidases of the o-type in membranes of the methanol-grown obligate methylotroph Methylobacillus flagellatum KT were distinguished. For this purpose the kinetic analysis of the laser flash-induced optical absorbance changes of CO-oxidase complexes under reducing conditions was used. The ratio of these oxidases in membranes greatly depended on th...

Cells of type I obligate methylotroph Methylobacillus flagellatum KT, which can use only methanol or methylamine as carbon and energy sources, possess two types of blue copper proteins: amicyanin and azurin. During exponential phase of growth amicyanin was the only blue copper-containing protein in the cells from methylamine medium. Azurin-type pro...