Tan Shiyong

Publications (2) View all

• Article: Antagonistic bacterium Bacillus amyloliquefaciens induces resistance and controls the bacterial wilt of tomato.

  Shiyong Tan, Yue Dong, Hanpeng Liao, Jianfeng Huang, Song Song, Yangchun Xu, Qirong Shen
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  ABSTRACT: BACKGROUND: Bacterial wilt caused by Ralstonia solanacearum (RS) is a serious threat for agricultural production. In this study, Bacillus amyloliquefaciens strains CM-2 and T-5 antagonistic to RS were used to create bioorganic fertilisers to control tomato wilt under greenhouse conditions. The possible mechanism of resistance inducement by the antagonistic bacteria was also evaluated. RESULTS: The application of bioorganic fertilisers significantly reduced incidences of tomato wilt (by 63-74%), promoted plant growth and significantly reduced the RS populations in rhizosphere compared with the control. Both strains CM-2 and T-5 applied with bioorganic fertilisers survived well in the tomato rhizosphere. Tomato seedlings treated with cell suspension of T-5 followed by challenge inoculation with RS increased the activities of polyphenol oxidase, phenylalanine ammonia lyase and peroxidase compared with the untreated control. Furthermore, the expressions of the marker genes responsible for synthesis of phytohormones salicylic acid, ethylene and jasmonic acid in seedlings treated with T-5 in response to inoculated pathogen were significantly higher. CONCLUSIONS: This study suggests that strains CM-2 and T-5 containing bioorganic fertilisers effectively control tomato wilt. Increased enzyme activities and expression of defence genes in plants indicated that the antagonistic bacteria induced plant resistance, which was the potential biocontrol mechanism of tomato wilt. © 2013 Society of Chemical Industry.
  Pest Management Science 01/2013; · 2.25 Impact Factor
• Article: Production and characterization of acidophilic xylanolytic enzymes from Penicillium oxalicum GZ-2.

  Hanpeng Liao, Chunmiao Xu, Shiyong Tan, Zhong Wei, Ning Ling, Guanghui Yu, Waseem Raza, Ruifu Zhang, Qirong Shen, Yangchun Xu
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  ABSTRACT: Multiple acidophilic xylanolytic enzymes were produced by Penicillium oxalicum GZ-2 during growth on wheat straw, rice straw, corn stover, and wheat bran. The expression of xylanase isoforms was dependent on substrate type and nitrogen source. The zymograms produced by the SDS-PAGE resolution of the crude enzymes indicated that wheat straw was the best inducer, resulting in the highest xylanase (115.2U/mL) and β-xylosidase (89mU/mL) activities during submerged fermentation. The optimum temperature and pH for xylanase activity were 50°C and 4.0, respectively; however, the crude xylanase enzymes exhibited remarkable stability over a broad pH range and showed more than 90% activity at 50°C for 30min at pH 4.0-8.0. The results revealed that P. oxalicum GZ-2 is a promising acidophilic xylanase-producing microorganism that has great potential to be used in biofuels, animal feed, and food industry applications.
  Bioresource technology 07/2012; 123:117-24. · 4.25 Impact Factor