Tal Etzyoni

Questions and Answers (25) View all

• Answer added in Gene Expression and Chromatin Biology
  15 What is the time it takes to repair double-strand breaks (DSBs)?
  By Tal Etzyoni · Tel Aviv University
  Tal Etzyoni · Tel Aviv University

  Dear Aditi, Thanks for your reply. I cause the DSBs by alpha irradiation. I performed a time course of 10 mins to 40 hours. I looked at immunefloure... [more]

  Dear Aditi, Thanks for your reply. I cause the DSBs by alpha irradiation. I performed a time course of 10 mins to 40 hours. I looked at immuneflourescence slides using anti-ku70 and DAPI. The weird thing is that I saw nuclear ku70 after 40 hours and looked in the literature to see if anyone got it before me, but could not find it.
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• Question asked in Gene Expression and Chromatin Biology
  15 What is the time it takes to repair double-strand breaks (DSBs)?
  Is anyone familiar with the time it takes to repair double-strand breaks (DSBs)? I realize it depends on the breaks' complexity but I was wondering if... [more]
  Is anyone familiar with the time it takes to repair double-strand breaks (DSBs)? I realize it depends on the breaks' complexity but I was wondering if it is possible to see ku70 in the nucleus several hours post insult, and what happends to ku70 when the damage is repaired? Does it stay in the nucleus or goes back to the cytoplasm?
  By Tal Etzyoni · Tel Aviv University
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• Answer added in Western Blot
  7 Different widths on Western Blot
  By Tal Etzyoni · Tel Aviv University
  Tal Etzyoni · Tel Aviv University

  Thank you all! the gel are prepared 1-3 days before the assay so this is probably not the issue. The samples are in the same buffers, so this is too... [more]

  Thank you all! the gel are prepared 1-3 days before the assay so this is probably not the issue. The samples are in the same buffers, so this is too may not the problem... You are right that after all the amount is calculated in a densitometric software so the differences are diminished. Thank again Tali
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• Question asked in Western Blot
  7 Different widths on Western Blot
  Why do I get different width in different samples althought I loaded the same amount? 
  Why do I get different width in different samples althought I loaded the same amount?
  

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  By Tal Etzyoni · Tel Aviv University
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• Answer added in Western Blot
  3 What is the correct way to substract background? a straight line or a line that is connected at the bottom of the peaks?
  By Tal Etzyoni · Tel Aviv University
  Tal Etzyoni · Tel Aviv University

  Thanks a LOT! 

  Thanks a LOT!
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