Tadele Tolosa

Publications (50) View all

• Source

  Available from: Tadele Tolosa

  Article: Comparative evaluation of the Rose Bengal plate test, standard tube agglutination test and complement fixation test for the diagnosis of human brucellosis

  M. Yohannes, J.P.S. Gill, S. Ghatak, D.K. Singh, T. Tolosa
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  ABSTRACT: In this study, 241 serum samples from individuals exposed to brucellosis were subjected to the Rose Bengal plate test (RBPT); the titre was estimated by standard tube agglutination test (STAT), with positive ≥ 80 IU/ml. Randomly selected sera (n = 81) were analysed by complement fixation test (CFT): titre ≥ 1:4 was considered positive. Of 241 sera subjected to RBPT and STAT, 177 were negative in both tests; 5 samples tested negative by RBPT but positive by STAT. None was positive by RBPT and negative by STAT. Of 81 sera subjected to CFT, 23 (28.4%) were positive. Both RBPT and CFT found 18 samples positive; 5 samples were positive by CFT and negative by RBPT. Comparison of STAT with CFT showed 13 samples positive by STAT but negative by CFT, and 4 positive by CFT but negative by STAT. The sensitivity and specificity of STAT were 82.6% and 77.6%, respectively, with CFT as gold standard. No test is perfect, and the clinical history coupled with a combination of two or more tests will reduce diagnostic errors.
  Revue scientifique et technique (International Office of Epizootics) 12/2012; 31(3):979-984. · 1.10 Impact Factor
• Article: Prevalence study on bovine tuberculosis and molecular characterization of its causative agents in cattle slaughtered at Addis Ababa municipal abattoir, Central Ethiopia.

  Abraham Mekibeb, Tadele Tolosa Fulasa, Rebuma Firdessa, Elena Hailu
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  ABSTRACT: A cross-sectional study was conducted on 500 cattle slaughtered at Addis Ababa abattoir to determine the prevalence of bovine tuberculosis (BTB) and characterize its causative agents. Postmortem examination, mycobacteriological culturing, region of difference-4 (RD4)-based PCR and spoligotyping were applied. The prevalence of BTB was 5 % on the basis of postmortem inspection alone but 1.2 % based on molecular confirmation. Factors including age, sex, and breed showed statistically significant association with BTB (p < 0.05). Gross lesions were observed most frequently (68 %) in the lungs and lung-associated lymph nodes compared to other organs and lymph nodes. Of the 25 grossly suspicious TB lesions processed and cultured, only six (24 %) were culture-positive, yielding Mycobacterium bovis confirmed by RD4 deletion typing. Further characterization of the six M. bovis isolates at the strain level by using spoligotyping revealed that one did not belong to any previously known type, while the others belonged to types SB1176 (two), SB1477 (two), and SB0133 (one). The new strain was submitted to the international M. Bovis.org database for international code designation. The study confirms the considerable prevalence of bovine tuberculosis in cattle slaughtered at Addis Ababa abattoir and highlights the need for control of bovine tuberculosis in the country.
  Tropical Animal Health and Production 10/2012; · 1.12 Impact Factor
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  Available from: Tadele Tolosa

  Article: Brucellosis in Ethiopia- A Review

  Moti Yohannes, Hailu Degefu, Tadele Tolosa, Kelay Belihu, Ronald Cutler, Sally Cutler
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  ABSTRACT: We reviewed the distribution of brucellosis in different regions of Ethiopia and its prevalence among different livestock hosts. In the absence of recent published documents, unpublished studies were cited to provide some information on distribution and importance of brucellosis in Ethiopia. Risk factors for the occurrence of brucellosis are also reviewed. Finally, different strategies for the control and prevention of brucellosis under Ethiopian conditions are discussed.
  African journal of microbiology research 04/2013; 7(14):1150-1157. · 0.54 Impact Factor
• Article: Seroprevalence of Brucellosis and Q-Fever in Southeast Ethiopian Pastoral Livestock

  Balako Gumi, Rebuma Firdessa, Lawrence Yamuah, Teshale Sori, Tadele Tolosa, Abraham Aseffa, Jakob Zinsstag, Esther Schelling
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  ABSTRACT: To assess seroprevalences of Brucellaand C. burnetiiin pastoral livestock in southeast Ethiopia, a cross-sectional study was carried out in three livestock species (cattle, camels and goats). The study was conducted from July 2008 to August 2010, and eight pastoral associations (PAs) from the selected districts were included in the study. Sera from a total of 1830 animals, comprising 862 cattle, 458 camels and 510 goats were screened initially with Rose Bengal plate test (RBPT) for Brucella. All RBPT positive and 25% of randomly selected negative sera were further tested by ELISA. These comprise a total of 460 animals (211 cattle, 102 camels and 147 goats).
  Journal of Veterinary Science & Medical Diagnosis. 01/2013; 2(1).
• Article: Human taenisis and bovine cysticercosis in selected health centers and abattoirs in Modjo and Addis Ababa, Ethiopia

  Deressa A, Yohannes M, Alemayehu M, Degefu H, Tolosa T, Pal M
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  ABSTRACT: Taeniasis and bovine cysticercosis are common parasitic infections in developing countries like Ethiopia. The purpose of this study was to know the prevalence of taeniasis and Cysticercus bovis using active and retrospective assessment in Addis Ababa and Modjo from November 2009 to March 2010. For this cross-sectional study stool samples were collected from Saint Paul Hospital, Ethiopian Health and Nutrition Research Institute and clinics at Addis Ababa Abattoir and Luna Export Abattoir. The stool samples were examined for the presence of Taenia spp eggs microscopically and cattle slaughtered during the study period were also subjected to postmortem inspection to study bovine cysticercosis. Case book records were also assessed to get information on situation of taeniasis and bovine cysticercosis. Out of 384 stool samples examined, the overall prevalence of Taenia spp. egg was 8.1%. The prevalence at different stool sampling sites was not found statistically significant (P>0.05). Taenia saginata infection was found higher among age group 26-30 years followed by individuals with greater than 40 years of age (P<0.05). The sex-wise prevalence was 8.4% and 7.8% in female and male (P>0.05), respectively. From a total of 61000 cattle carcass inspected using active abattoir survey of both sites, 3.7% were found positive for C. bovis. On the basis of retrospective survey, of 366 total stool samples collected throughout the year and the prevalence of Taenia spp. egg was 6.6%. Age and sex-wise Taenia spp. egg were observed more in 26-30 years age categories as compared to less than 15 years age categories. Of a total 33289 cattle slaughtered between 2001 and 2008 at Addis Ababa abattoir 1.04% had C. bovis cyst. The results of the present study clearly indicated the economic as well as public health significances of the infection. Thus, interdisciplinary collaboration between veterinarians and medical personnel is imperative to help in the control of this infection.
  International Journal of Livestock Research Online. 01/2012; 2(2):206-216.